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Seed Biopriming With Trichoderma Strains Isolated From Tree Bark Improves Plant Growth, Antioxidative Defense System in Rice and Enhance Straw Degradation Capacity

This study is a unique report of the utilization of Trichoderma strains collected from even tree barks for rice plant growth, its health management, and paddy straw degradation. Seven different spp. of Trichoderma were characterized according to morphological and molecular tools. Two of the isolated...

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Autores principales: Swain, Harekrushna, Adak, Totan, Mukherjee, Arup K., Sarangi, Sarmistha, Samal, Pankajini, Khandual, Ansuman, Jena, Rupalin, Bhattacharyya, Pratap, Naik, Soumendra K., Mehetre, Sayaji T., Baite, Mathew S., Kumar M, Sunil, Zaidi, Najam Waris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952651/
https://www.ncbi.nlm.nih.gov/pubmed/33717027
http://dx.doi.org/10.3389/fmicb.2021.633881
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author Swain, Harekrushna
Adak, Totan
Mukherjee, Arup K.
Sarangi, Sarmistha
Samal, Pankajini
Khandual, Ansuman
Jena, Rupalin
Bhattacharyya, Pratap
Naik, Soumendra K.
Mehetre, Sayaji T.
Baite, Mathew S.
Kumar M, Sunil
Zaidi, Najam Waris
author_facet Swain, Harekrushna
Adak, Totan
Mukherjee, Arup K.
Sarangi, Sarmistha
Samal, Pankajini
Khandual, Ansuman
Jena, Rupalin
Bhattacharyya, Pratap
Naik, Soumendra K.
Mehetre, Sayaji T.
Baite, Mathew S.
Kumar M, Sunil
Zaidi, Najam Waris
author_sort Swain, Harekrushna
collection PubMed
description This study is a unique report of the utilization of Trichoderma strains collected from even tree barks for rice plant growth, its health management, and paddy straw degradation. Seven different spp. of Trichoderma were characterized according to morphological and molecular tools. Two of the isolated strains, namely Trichoderma hebeiensis and Trichoderma erinaceum, outperformed the other strains. Both of the strains controlled four important rice pathogens, i.e., Rhizoctonia solani (100%), Sclerotium oryzae (84.17%), Sclerotium rolfsii (66.67%), and Sclerotium delphinii (76.25%). Seed bio-priming with respective Trichoderma strains reduced the mean germination time, enhanced the seedling vigor and total chlorophyll content which could be related to the higher yield observed in two rice varieties; Annapurna and Satabdi. All the seven strains accelerated the decomposition of rice straw by producing higher straw degrading enzymes like total cellulase (0.97–2.59 IU/mL), endoglucanase (0.53–0.75 IU/mL), xylanase (145.35–201.35 nkat/mL), and laccase (2.48–12.60 IU/mL). They also produced higher quantities of indole acetic acid (19.19–46.28 μg/mL), soluble phosphate (297.49–435.42 μg/mL), and prussic acid (0.01–0.37 μg/mL) which are responsible for plant growth promotion and the inhibition of rice pathogen populations. Higher expression of defense enzymes like catalase (≥250% both in shoot and root), peroxidase (≥150% in root and ≥100% in shoot), superoxide dismutase (≥ 150% in root and ≥100% in shoot), polyphenol oxidase (≥160% in shoot and ≥120% in shoot), and total phenolics (≥200% in root and ≥250% in shoot) as compared to the control indicates stress tolerance ability to rice crop. The expression of the aforementioned enzymes were confirmed by the expression of corresponding defense genes like PAL (>3-fold), DEFENSIN (>1-fold), POX (>1.5-fold), LOX (>1-fold), and PR-3 (>2-fold) as compared to the non-treated control plants. This investigation demonstrates that Trichoderma strains obtained from tree bark could be considered to be utilized for the sustainable health management of rice crop.
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spelling pubmed-79526512021-03-13 Seed Biopriming With Trichoderma Strains Isolated From Tree Bark Improves Plant Growth, Antioxidative Defense System in Rice and Enhance Straw Degradation Capacity Swain, Harekrushna Adak, Totan Mukherjee, Arup K. Sarangi, Sarmistha Samal, Pankajini Khandual, Ansuman Jena, Rupalin Bhattacharyya, Pratap Naik, Soumendra K. Mehetre, Sayaji T. Baite, Mathew S. Kumar M, Sunil Zaidi, Najam Waris Front Microbiol Microbiology This study is a unique report of the utilization of Trichoderma strains collected from even tree barks for rice plant growth, its health management, and paddy straw degradation. Seven different spp. of Trichoderma were characterized according to morphological and molecular tools. Two of the isolated strains, namely Trichoderma hebeiensis and Trichoderma erinaceum, outperformed the other strains. Both of the strains controlled four important rice pathogens, i.e., Rhizoctonia solani (100%), Sclerotium oryzae (84.17%), Sclerotium rolfsii (66.67%), and Sclerotium delphinii (76.25%). Seed bio-priming with respective Trichoderma strains reduced the mean germination time, enhanced the seedling vigor and total chlorophyll content which could be related to the higher yield observed in two rice varieties; Annapurna and Satabdi. All the seven strains accelerated the decomposition of rice straw by producing higher straw degrading enzymes like total cellulase (0.97–2.59 IU/mL), endoglucanase (0.53–0.75 IU/mL), xylanase (145.35–201.35 nkat/mL), and laccase (2.48–12.60 IU/mL). They also produced higher quantities of indole acetic acid (19.19–46.28 μg/mL), soluble phosphate (297.49–435.42 μg/mL), and prussic acid (0.01–0.37 μg/mL) which are responsible for plant growth promotion and the inhibition of rice pathogen populations. Higher expression of defense enzymes like catalase (≥250% both in shoot and root), peroxidase (≥150% in root and ≥100% in shoot), superoxide dismutase (≥ 150% in root and ≥100% in shoot), polyphenol oxidase (≥160% in shoot and ≥120% in shoot), and total phenolics (≥200% in root and ≥250% in shoot) as compared to the control indicates stress tolerance ability to rice crop. The expression of the aforementioned enzymes were confirmed by the expression of corresponding defense genes like PAL (>3-fold), DEFENSIN (>1-fold), POX (>1.5-fold), LOX (>1-fold), and PR-3 (>2-fold) as compared to the non-treated control plants. This investigation demonstrates that Trichoderma strains obtained from tree bark could be considered to be utilized for the sustainable health management of rice crop. Frontiers Media S.A. 2021-02-26 /pmc/articles/PMC7952651/ /pubmed/33717027 http://dx.doi.org/10.3389/fmicb.2021.633881 Text en Copyright © 2021 Swain, Adak, Mukherjee, Sarangi, Samal, Khandual, Jena, Bhattacharyya, Naik, Mehetre, Baite, Kumar M and Zaidi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Swain, Harekrushna
Adak, Totan
Mukherjee, Arup K.
Sarangi, Sarmistha
Samal, Pankajini
Khandual, Ansuman
Jena, Rupalin
Bhattacharyya, Pratap
Naik, Soumendra K.
Mehetre, Sayaji T.
Baite, Mathew S.
Kumar M, Sunil
Zaidi, Najam Waris
Seed Biopriming With Trichoderma Strains Isolated From Tree Bark Improves Plant Growth, Antioxidative Defense System in Rice and Enhance Straw Degradation Capacity
title Seed Biopriming With Trichoderma Strains Isolated From Tree Bark Improves Plant Growth, Antioxidative Defense System in Rice and Enhance Straw Degradation Capacity
title_full Seed Biopriming With Trichoderma Strains Isolated From Tree Bark Improves Plant Growth, Antioxidative Defense System in Rice and Enhance Straw Degradation Capacity
title_fullStr Seed Biopriming With Trichoderma Strains Isolated From Tree Bark Improves Plant Growth, Antioxidative Defense System in Rice and Enhance Straw Degradation Capacity
title_full_unstemmed Seed Biopriming With Trichoderma Strains Isolated From Tree Bark Improves Plant Growth, Antioxidative Defense System in Rice and Enhance Straw Degradation Capacity
title_short Seed Biopriming With Trichoderma Strains Isolated From Tree Bark Improves Plant Growth, Antioxidative Defense System in Rice and Enhance Straw Degradation Capacity
title_sort seed biopriming with trichoderma strains isolated from tree bark improves plant growth, antioxidative defense system in rice and enhance straw degradation capacity
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952651/
https://www.ncbi.nlm.nih.gov/pubmed/33717027
http://dx.doi.org/10.3389/fmicb.2021.633881
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