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ZmRAD17 Is Required for Accurate Double-Strand Break Repair During Maize Male Meiosis

RAD17, a replication factor C (RFC)-like DNA damage sensor protein, is involved in DNA checkpoint control and required for both meiosis and mitosis in yeast and mammals. In plant, the meiotic function of RAD17 was only reported in rice so far. Here, we identified and characterized the RAD17 homolog...

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Autores principales: Zhang, Ting, Jing, Ju-Li, Liu, Lei, He, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952653/
https://www.ncbi.nlm.nih.gov/pubmed/33719299
http://dx.doi.org/10.3389/fpls.2021.626528
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author Zhang, Ting
Jing, Ju-Li
Liu, Lei
He, Yan
author_facet Zhang, Ting
Jing, Ju-Li
Liu, Lei
He, Yan
author_sort Zhang, Ting
collection PubMed
description RAD17, a replication factor C (RFC)-like DNA damage sensor protein, is involved in DNA checkpoint control and required for both meiosis and mitosis in yeast and mammals. In plant, the meiotic function of RAD17 was only reported in rice so far. Here, we identified and characterized the RAD17 homolog in maize. The Zmrad17 mutants exhibited normal vegetative growth but male was partially sterile. In Zmrad17 pollen mother cells, non-homologous chromosome entanglement and chromosome fragmentation were frequently observed. Immunofluorescence analysis manifested that DSB formation occurred as normal and the loading pattern of RAD51 signals was similar to wild-type at the early stage of prophase I in the mutants. The localization of the axial element ASY1 was normal, while the assembly of the central element ZYP1 was severely disrupted in Zmrad17 meiocytes. Surprisingly, no obvious defect in female sterility was observed in Zmrad17 mutants. Taken together, our results suggest that ZmRAD17 is involved in DSB repair likely by promoting synaptonemal complex assembly in maize male meiosis. These phenomena highlight a high extent of divergence from its counterpart in rice, indicating that the RAD17 dysfunction can result in a drastic dissimilarity in meiotic outcome in different plant species.
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spelling pubmed-79526532021-03-13 ZmRAD17 Is Required for Accurate Double-Strand Break Repair During Maize Male Meiosis Zhang, Ting Jing, Ju-Li Liu, Lei He, Yan Front Plant Sci Plant Science RAD17, a replication factor C (RFC)-like DNA damage sensor protein, is involved in DNA checkpoint control and required for both meiosis and mitosis in yeast and mammals. In plant, the meiotic function of RAD17 was only reported in rice so far. Here, we identified and characterized the RAD17 homolog in maize. The Zmrad17 mutants exhibited normal vegetative growth but male was partially sterile. In Zmrad17 pollen mother cells, non-homologous chromosome entanglement and chromosome fragmentation were frequently observed. Immunofluorescence analysis manifested that DSB formation occurred as normal and the loading pattern of RAD51 signals was similar to wild-type at the early stage of prophase I in the mutants. The localization of the axial element ASY1 was normal, while the assembly of the central element ZYP1 was severely disrupted in Zmrad17 meiocytes. Surprisingly, no obvious defect in female sterility was observed in Zmrad17 mutants. Taken together, our results suggest that ZmRAD17 is involved in DSB repair likely by promoting synaptonemal complex assembly in maize male meiosis. These phenomena highlight a high extent of divergence from its counterpart in rice, indicating that the RAD17 dysfunction can result in a drastic dissimilarity in meiotic outcome in different plant species. Frontiers Media S.A. 2021-02-26 /pmc/articles/PMC7952653/ /pubmed/33719299 http://dx.doi.org/10.3389/fpls.2021.626528 Text en Copyright © 2021 Zhang, Jing, Liu and He. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Ting
Jing, Ju-Li
Liu, Lei
He, Yan
ZmRAD17 Is Required for Accurate Double-Strand Break Repair During Maize Male Meiosis
title ZmRAD17 Is Required for Accurate Double-Strand Break Repair During Maize Male Meiosis
title_full ZmRAD17 Is Required for Accurate Double-Strand Break Repair During Maize Male Meiosis
title_fullStr ZmRAD17 Is Required for Accurate Double-Strand Break Repair During Maize Male Meiosis
title_full_unstemmed ZmRAD17 Is Required for Accurate Double-Strand Break Repair During Maize Male Meiosis
title_short ZmRAD17 Is Required for Accurate Double-Strand Break Repair During Maize Male Meiosis
title_sort zmrad17 is required for accurate double-strand break repair during maize male meiosis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952653/
https://www.ncbi.nlm.nih.gov/pubmed/33719299
http://dx.doi.org/10.3389/fpls.2021.626528
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