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Phylogenetic analysis of the neuraminidase segment gene of Influenza A/H1N1 strains isolated from Monastir Region (Tunisia) during the 2017–2018 outbreak
Influenza A/H1N1 is widely considered to be a very evolutionary virus causing major public health problems. Since the pandemic of 2009, there has been a rapid rise in human Influenza virus characterization. However, little data is available in Tunisia regarding its genetic evolution. In light of thi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952816/ https://www.ncbi.nlm.nih.gov/pubmed/33727729 http://dx.doi.org/10.1007/s11756-021-00723-y |
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author | Ben Hamed, Sabrine Elargoubi, Aida Harrabi, Myriam Srihi, Haythem Souiai, Oussema Mastouri, Maha Almalki, Mohammed Awadh Gharbi, Jawhar Ben M’hadheb, Manel |
author_facet | Ben Hamed, Sabrine Elargoubi, Aida Harrabi, Myriam Srihi, Haythem Souiai, Oussema Mastouri, Maha Almalki, Mohammed Awadh Gharbi, Jawhar Ben M’hadheb, Manel |
author_sort | Ben Hamed, Sabrine |
collection | PubMed |
description | Influenza A/H1N1 is widely considered to be a very evolutionary virus causing major public health problems. Since the pandemic of 2009, there has been a rapid rise in human Influenza virus characterization. However, little data is available in Tunisia regarding its genetic evolution. In light of this fact, our paper aim is to genetically characterize the Neuraminidase, known as the target of antiviral inhibitors, in Tunisian isolates circulating in Monastir region during 2017–2018. In total of 31 positive Influenza A/H1N1 detected by multiplex real-time PCR, RT-PCR of neuraminidase was performed. Among the 31 positive samples, 7 samples representing fatal and most severe cases were conducted for sequencing and genetic analysis. The results thus obtained showed genetic evolution of the A/H1N1 neuraminidase between 2009 and 2010 and 2018–2019 outbreaks. All Tunisian isolates were genetically related to the recommended vaccine strain with a specific evolution. Moreover, the phylogenetic analysis demonstrated that France and especially Italian strains were the major related strains. Interestingly, our results revealed a specific cluster of Tunisian isolates where two intragroup were evolved in correlation with the severity and the fatalities cases. From the outcome of our investigation, this study confirms the genetic evolution of the Influenza A virus circulating in Tunisia and gives a preliminary analysis for a better comprehension of new emerging Tunisian strain’s virulence and thus, a more appropriate monitoring of Influenza virus A/H1N1 during each round of outbreaks. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11756-021-00723-y. |
format | Online Article Text |
id | pubmed-7952816 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-79528162021-03-12 Phylogenetic analysis of the neuraminidase segment gene of Influenza A/H1N1 strains isolated from Monastir Region (Tunisia) during the 2017–2018 outbreak Ben Hamed, Sabrine Elargoubi, Aida Harrabi, Myriam Srihi, Haythem Souiai, Oussema Mastouri, Maha Almalki, Mohammed Awadh Gharbi, Jawhar Ben M’hadheb, Manel Biologia (Bratisl) Original Article Influenza A/H1N1 is widely considered to be a very evolutionary virus causing major public health problems. Since the pandemic of 2009, there has been a rapid rise in human Influenza virus characterization. However, little data is available in Tunisia regarding its genetic evolution. In light of this fact, our paper aim is to genetically characterize the Neuraminidase, known as the target of antiviral inhibitors, in Tunisian isolates circulating in Monastir region during 2017–2018. In total of 31 positive Influenza A/H1N1 detected by multiplex real-time PCR, RT-PCR of neuraminidase was performed. Among the 31 positive samples, 7 samples representing fatal and most severe cases were conducted for sequencing and genetic analysis. The results thus obtained showed genetic evolution of the A/H1N1 neuraminidase between 2009 and 2010 and 2018–2019 outbreaks. All Tunisian isolates were genetically related to the recommended vaccine strain with a specific evolution. Moreover, the phylogenetic analysis demonstrated that France and especially Italian strains were the major related strains. Interestingly, our results revealed a specific cluster of Tunisian isolates where two intragroup were evolved in correlation with the severity and the fatalities cases. From the outcome of our investigation, this study confirms the genetic evolution of the Influenza A virus circulating in Tunisia and gives a preliminary analysis for a better comprehension of new emerging Tunisian strain’s virulence and thus, a more appropriate monitoring of Influenza virus A/H1N1 during each round of outbreaks. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11756-021-00723-y. Springer International Publishing 2021-03-12 2021 /pmc/articles/PMC7952816/ /pubmed/33727729 http://dx.doi.org/10.1007/s11756-021-00723-y Text en © Slovak Academy of Sciences 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Ben Hamed, Sabrine Elargoubi, Aida Harrabi, Myriam Srihi, Haythem Souiai, Oussema Mastouri, Maha Almalki, Mohammed Awadh Gharbi, Jawhar Ben M’hadheb, Manel Phylogenetic analysis of the neuraminidase segment gene of Influenza A/H1N1 strains isolated from Monastir Region (Tunisia) during the 2017–2018 outbreak |
title | Phylogenetic analysis of the neuraminidase segment gene of Influenza A/H1N1 strains isolated from Monastir Region (Tunisia) during the 2017–2018 outbreak |
title_full | Phylogenetic analysis of the neuraminidase segment gene of Influenza A/H1N1 strains isolated from Monastir Region (Tunisia) during the 2017–2018 outbreak |
title_fullStr | Phylogenetic analysis of the neuraminidase segment gene of Influenza A/H1N1 strains isolated from Monastir Region (Tunisia) during the 2017–2018 outbreak |
title_full_unstemmed | Phylogenetic analysis of the neuraminidase segment gene of Influenza A/H1N1 strains isolated from Monastir Region (Tunisia) during the 2017–2018 outbreak |
title_short | Phylogenetic analysis of the neuraminidase segment gene of Influenza A/H1N1 strains isolated from Monastir Region (Tunisia) during the 2017–2018 outbreak |
title_sort | phylogenetic analysis of the neuraminidase segment gene of influenza a/h1n1 strains isolated from monastir region (tunisia) during the 2017–2018 outbreak |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952816/ https://www.ncbi.nlm.nih.gov/pubmed/33727729 http://dx.doi.org/10.1007/s11756-021-00723-y |
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