Cargando…

Towards a sensitive and accurate interpretation of molecular testing for SARS-CoV-2: a rapid review of 264 studies

BACKGROUND: Sensitive molecular diagnostics and correct test interpretation are crucial for accurate COVID-19 diagnosis and thereby essential for good clinical practice. Furthermore, they are a key factor in outbreak control where active case finding in combination with isolation and contact tracing...

Descripción completa

Detalles Bibliográficos
Autores principales: Stanoeva, Kamelia R., van der Eijk, Annemiek A., Meijer, Adam, Kortbeek, Laetitia M., Koopmans, Marion P.G., Reusken, Chantal B.E.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953531/
https://www.ncbi.nlm.nih.gov/pubmed/33706863
http://dx.doi.org/10.2807/1560-7917.ES.2021.26.10.2001134
_version_ 1783663936532381696
author Stanoeva, Kamelia R.
van der Eijk, Annemiek A.
Meijer, Adam
Kortbeek, Laetitia M.
Koopmans, Marion P.G.
Reusken, Chantal B.E.M.
author_facet Stanoeva, Kamelia R.
van der Eijk, Annemiek A.
Meijer, Adam
Kortbeek, Laetitia M.
Koopmans, Marion P.G.
Reusken, Chantal B.E.M.
author_sort Stanoeva, Kamelia R.
collection PubMed
description BACKGROUND: Sensitive molecular diagnostics and correct test interpretation are crucial for accurate COVID-19 diagnosis and thereby essential for good clinical practice. Furthermore, they are a key factor in outbreak control where active case finding in combination with isolation and contact tracing are crucial. AIM: With the objective to inform the public health and laboratory responses to the pandemic, we reviewed current published knowledge on the kinetics of SARS-CoV-2 infection as assessed by RNA molecular detection in a wide range of clinical samples. METHODS: We performed an extensive search on studies published between 1 December 2019 and 15 May 2020, reporting on molecular detection and/or isolation of SARS-CoV-2 in any human laboratory specimen. RESULTS: We compiled a dataset of 264 studies including 32,515 COVID-19 cases, and additionally aggregated data points (n = 2,777) from sampling of 217 adults with known infection timeline. We summarised data on SARS-CoV-2 detection in the respiratory and gastrointestinal tract, blood, oral fluid, tears, cerebrospinal fluid, peritoneal fluid, semen, vaginal fluid; where provided, we also summarised specific observations on SARS-CoV-2 detection in pregnancy, infancy, children, adolescents and immunocompromised individuals. CONCLUSION: Optimal SARS-CoV-2 molecular testing relies on choosing the most appropriate sample type, collected with adequate sampling technique, and with the infection timeline in mind. We outlined knowledge gaps and directions for future well-documented systematic studies.
format Online
Article
Text
id pubmed-7953531
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher European Centre for Disease Prevention and Control (ECDC)
record_format MEDLINE/PubMed
spelling pubmed-79535312021-03-18 Towards a sensitive and accurate interpretation of molecular testing for SARS-CoV-2: a rapid review of 264 studies Stanoeva, Kamelia R. van der Eijk, Annemiek A. Meijer, Adam Kortbeek, Laetitia M. Koopmans, Marion P.G. Reusken, Chantal B.E.M. Euro Surveill Review BACKGROUND: Sensitive molecular diagnostics and correct test interpretation are crucial for accurate COVID-19 diagnosis and thereby essential for good clinical practice. Furthermore, they are a key factor in outbreak control where active case finding in combination with isolation and contact tracing are crucial. AIM: With the objective to inform the public health and laboratory responses to the pandemic, we reviewed current published knowledge on the kinetics of SARS-CoV-2 infection as assessed by RNA molecular detection in a wide range of clinical samples. METHODS: We performed an extensive search on studies published between 1 December 2019 and 15 May 2020, reporting on molecular detection and/or isolation of SARS-CoV-2 in any human laboratory specimen. RESULTS: We compiled a dataset of 264 studies including 32,515 COVID-19 cases, and additionally aggregated data points (n = 2,777) from sampling of 217 adults with known infection timeline. We summarised data on SARS-CoV-2 detection in the respiratory and gastrointestinal tract, blood, oral fluid, tears, cerebrospinal fluid, peritoneal fluid, semen, vaginal fluid; where provided, we also summarised specific observations on SARS-CoV-2 detection in pregnancy, infancy, children, adolescents and immunocompromised individuals. CONCLUSION: Optimal SARS-CoV-2 molecular testing relies on choosing the most appropriate sample type, collected with adequate sampling technique, and with the infection timeline in mind. We outlined knowledge gaps and directions for future well-documented systematic studies. European Centre for Disease Prevention and Control (ECDC) 2021-03-11 /pmc/articles/PMC7953531/ /pubmed/33706863 http://dx.doi.org/10.2807/1560-7917.ES.2021.26.10.2001134 Text en This article is copyright of the authors or their affiliated institutions, 2021. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.
spellingShingle Review
Stanoeva, Kamelia R.
van der Eijk, Annemiek A.
Meijer, Adam
Kortbeek, Laetitia M.
Koopmans, Marion P.G.
Reusken, Chantal B.E.M.
Towards a sensitive and accurate interpretation of molecular testing for SARS-CoV-2: a rapid review of 264 studies
title Towards a sensitive and accurate interpretation of molecular testing for SARS-CoV-2: a rapid review of 264 studies
title_full Towards a sensitive and accurate interpretation of molecular testing for SARS-CoV-2: a rapid review of 264 studies
title_fullStr Towards a sensitive and accurate interpretation of molecular testing for SARS-CoV-2: a rapid review of 264 studies
title_full_unstemmed Towards a sensitive and accurate interpretation of molecular testing for SARS-CoV-2: a rapid review of 264 studies
title_short Towards a sensitive and accurate interpretation of molecular testing for SARS-CoV-2: a rapid review of 264 studies
title_sort towards a sensitive and accurate interpretation of molecular testing for sars-cov-2: a rapid review of 264 studies
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953531/
https://www.ncbi.nlm.nih.gov/pubmed/33706863
http://dx.doi.org/10.2807/1560-7917.ES.2021.26.10.2001134
work_keys_str_mv AT stanoevakameliar towardsasensitiveandaccurateinterpretationofmoleculartestingforsarscov2arapidreviewof264studies
AT vandereijkannemieka towardsasensitiveandaccurateinterpretationofmoleculartestingforsarscov2arapidreviewof264studies
AT meijeradam towardsasensitiveandaccurateinterpretationofmoleculartestingforsarscov2arapidreviewof264studies
AT kortbeeklaetitiam towardsasensitiveandaccurateinterpretationofmoleculartestingforsarscov2arapidreviewof264studies
AT koopmansmarionpg towardsasensitiveandaccurateinterpretationofmoleculartestingforsarscov2arapidreviewof264studies
AT reuskenchantalbem towardsasensitiveandaccurateinterpretationofmoleculartestingforsarscov2arapidreviewof264studies