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Single-Cell RNA Sequencing of the Adult Mammalian Heart—State-of-the-Art and Future Perspectives
PURPOSE OF THE REVIEW: Cardiovascular disease remains the leading cause of death worldwide, resulting in cardiac dysfunction and, subsequently, heart failure (HF). Single-cell RNA sequencing (scRNA-seq) is a rapidly developing tool for studying the transcriptional heterogeneity in both healthy and d...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer US
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954708/ https://www.ncbi.nlm.nih.gov/pubmed/33629280 http://dx.doi.org/10.1007/s11897-021-00504-3 |
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author | Gladka, Monika M. |
author_facet | Gladka, Monika M. |
author_sort | Gladka, Monika M. |
collection | PubMed |
description | PURPOSE OF THE REVIEW: Cardiovascular disease remains the leading cause of death worldwide, resulting in cardiac dysfunction and, subsequently, heart failure (HF). Single-cell RNA sequencing (scRNA-seq) is a rapidly developing tool for studying the transcriptional heterogeneity in both healthy and diseased hearts. Wide applications of techniques like scRNA-seq could significantly contribute to uncovering the molecular mechanisms involved in the onset and progression to HF and contribute to the development of new, improved therapies. This review discusses several studies that successfully applied scRNA-seq to the mouse and human heart using various methods of tissue processing and downstream analysis. RECENT FINDINGS: The application of scRNA-seq in the cardiovascular field is continuously expanding, providing new detailed insights into cardiac pathophysiology. SUMMARY: Increased understanding of cardiac pathophysiology on the single-cell level will contribute to the development of novel, more effective therapeutic strategies. Here, we summarise the possible application of scRNA-seq to the adult mammalian heart. |
format | Online Article Text |
id | pubmed-7954708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-79547082021-03-28 Single-Cell RNA Sequencing of the Adult Mammalian Heart—State-of-the-Art and Future Perspectives Gladka, Monika M. Curr Heart Fail Rep Translational Research in Heart Failure (E. Bertero, Section Editor) PURPOSE OF THE REVIEW: Cardiovascular disease remains the leading cause of death worldwide, resulting in cardiac dysfunction and, subsequently, heart failure (HF). Single-cell RNA sequencing (scRNA-seq) is a rapidly developing tool for studying the transcriptional heterogeneity in both healthy and diseased hearts. Wide applications of techniques like scRNA-seq could significantly contribute to uncovering the molecular mechanisms involved in the onset and progression to HF and contribute to the development of new, improved therapies. This review discusses several studies that successfully applied scRNA-seq to the mouse and human heart using various methods of tissue processing and downstream analysis. RECENT FINDINGS: The application of scRNA-seq in the cardiovascular field is continuously expanding, providing new detailed insights into cardiac pathophysiology. SUMMARY: Increased understanding of cardiac pathophysiology on the single-cell level will contribute to the development of novel, more effective therapeutic strategies. Here, we summarise the possible application of scRNA-seq to the adult mammalian heart. Springer US 2021-02-25 2021 /pmc/articles/PMC7954708/ /pubmed/33629280 http://dx.doi.org/10.1007/s11897-021-00504-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Translational Research in Heart Failure (E. Bertero, Section Editor) Gladka, Monika M. Single-Cell RNA Sequencing of the Adult Mammalian Heart—State-of-the-Art and Future Perspectives |
title | Single-Cell RNA Sequencing of the Adult Mammalian Heart—State-of-the-Art and Future Perspectives |
title_full | Single-Cell RNA Sequencing of the Adult Mammalian Heart—State-of-the-Art and Future Perspectives |
title_fullStr | Single-Cell RNA Sequencing of the Adult Mammalian Heart—State-of-the-Art and Future Perspectives |
title_full_unstemmed | Single-Cell RNA Sequencing of the Adult Mammalian Heart—State-of-the-Art and Future Perspectives |
title_short | Single-Cell RNA Sequencing of the Adult Mammalian Heart—State-of-the-Art and Future Perspectives |
title_sort | single-cell rna sequencing of the adult mammalian heart—state-of-the-art and future perspectives |
topic | Translational Research in Heart Failure (E. Bertero, Section Editor) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954708/ https://www.ncbi.nlm.nih.gov/pubmed/33629280 http://dx.doi.org/10.1007/s11897-021-00504-3 |
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