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Photooxidation-induced fluorescence amplification system for an ultra-sensitive enzyme-linked immunosorbent assay (ELISA)

This report suggests a method of enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). The PIFA utilized autocatalytic photooxidation of the chemifluorescent substrate, 10-acetyl 3,7-dihydroxyphenoxazine (ADHP, Amplex Red) to ampl...

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Detalles Bibliográficos
Autores principales: Heo, Youhee, Shin, Kwanwoo, Park, Min Cheol, Kang, Ji Yoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954804/
https://www.ncbi.nlm.nih.gov/pubmed/33712666
http://dx.doi.org/10.1038/s41598-021-85107-7