The case for using mapped exonic non-duplicate reads when reporting RNA-sequencing depth: examples from pediatric cancer datasets

BACKGROUND: The reproducibility of gene expression measured by RNA sequencing (RNA-Seq) is dependent on the sequencing depth. While unmapped or non-exonic reads do not contribute to gene expression quantification, duplicate reads contribute to the quantification but are not informative for reproduci...

Descripción completa

Detalles Bibliográficos
Autores principales: Beale, Holly C, Roger, Jacquelyn M, Cattle, Matthew A, McKay, Liam T, Thompson, Drew K A, Learned, Katrina, Lyle, A Geoffrey, Kephart, Ellen T, Currie, Rob, Lam, Du Linh, Sanders, Lauren, Pfeil, Jacob, Vivian, John, Bjork, Isabel, Salama, Sofie R, Haussler, David, Vaske, Olena M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955155/
https://www.ncbi.nlm.nih.gov/pubmed/33712853
http://dx.doi.org/10.1093/gigascience/giab011

Ejemplares similares

Ejemplares similares