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Determining insulin sensitivity from glucose tolerance tests in Iberian and landrace pigs

As insulin sensitivity may help to explain divergences in growth and body composition between native and modern breeds, metabolic responses to glucose infusion were measured using an intra-arterial glucose tolerance test (IAGTT). Iberian (n = 4) and Landrace (n = 5) barrows (47.0 ± 1.2 kg body weigh...

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Autores principales: Rodríguez-López, José Miguel, Lachica, Manuel, González-Valero, Lucrecia, Fernández-Fígares, Ignacio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955676/
https://www.ncbi.nlm.nih.gov/pubmed/33854837
http://dx.doi.org/10.7717/peerj.11014
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author Rodríguez-López, José Miguel
Lachica, Manuel
González-Valero, Lucrecia
Fernández-Fígares, Ignacio
author_facet Rodríguez-López, José Miguel
Lachica, Manuel
González-Valero, Lucrecia
Fernández-Fígares, Ignacio
author_sort Rodríguez-López, José Miguel
collection PubMed
description As insulin sensitivity may help to explain divergences in growth and body composition between native and modern breeds, metabolic responses to glucose infusion were measured using an intra-arterial glucose tolerance test (IAGTT). Iberian (n = 4) and Landrace (n = 5) barrows (47.0 ± 1.2 kg body weight (BW)), fitted with a permanent carotid artery catheter were injected with glucose (500 mg/kg BW) and blood samples collected at -10, 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, 120 and 180 min following glucose infusion. Plasma samples were analysed for insulin, glucose, lactate, triglycerides, cholesterol, creatinine, albumin and urea. Insulin sensitivity indices were calculated and analysed. Mean plasma glucose, creatinine and cholesterol concentrations were lower (P < 0.01) in Iberian (14, 68 and 22%, respectively) than in Landrace pigs during the IAGTT. However, mean plasma insulin, lactate, triglycerides and urea concentrations were greater (P < 0.001) in Iberian (50, 35, 18 and 23%, respectively) than in Landrace pigs. Iberian pigs had larger area under the curve (AUC) of insulin (P < 0.05) or tended to a greater AUC of lactate (P < 0.10), and a smaller (P < 0.05) AUC for glucose 0-60 min compared with Landrace pigs. Indices for estimating insulin sensitivity in fasting conditions indicated improved β-cell function in Iberian compared with Landrace pigs, but no difference (P > 0.10) in calculated insulin sensitivity index was found after IAGTT between breeds. A time response (P < 0.05) was obtained for insulin, glucose and lactate so that maximum concentration was achieved at 10 and 15 min post-infusion for insulin (Iberian and Landrace pigs, respectively), immediately post-infusion for glucose, and 20 min post-infusion for lactate, decreasing thereafter until basal levels. There was no time effect for the rest of metabolites evaluated. In conclusion, growing Iberian pigs challenged with an IAGTT showed changes in biochemical parameters and insulin response that may indicate an early stage of insulin resistance.
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spelling pubmed-79556762021-04-13 Determining insulin sensitivity from glucose tolerance tests in Iberian and landrace pigs Rodríguez-López, José Miguel Lachica, Manuel González-Valero, Lucrecia Fernández-Fígares, Ignacio PeerJ Agricultural Science As insulin sensitivity may help to explain divergences in growth and body composition between native and modern breeds, metabolic responses to glucose infusion were measured using an intra-arterial glucose tolerance test (IAGTT). Iberian (n = 4) and Landrace (n = 5) barrows (47.0 ± 1.2 kg body weight (BW)), fitted with a permanent carotid artery catheter were injected with glucose (500 mg/kg BW) and blood samples collected at -10, 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, 120 and 180 min following glucose infusion. Plasma samples were analysed for insulin, glucose, lactate, triglycerides, cholesterol, creatinine, albumin and urea. Insulin sensitivity indices were calculated and analysed. Mean plasma glucose, creatinine and cholesterol concentrations were lower (P < 0.01) in Iberian (14, 68 and 22%, respectively) than in Landrace pigs during the IAGTT. However, mean plasma insulin, lactate, triglycerides and urea concentrations were greater (P < 0.001) in Iberian (50, 35, 18 and 23%, respectively) than in Landrace pigs. Iberian pigs had larger area under the curve (AUC) of insulin (P < 0.05) or tended to a greater AUC of lactate (P < 0.10), and a smaller (P < 0.05) AUC for glucose 0-60 min compared with Landrace pigs. Indices for estimating insulin sensitivity in fasting conditions indicated improved β-cell function in Iberian compared with Landrace pigs, but no difference (P > 0.10) in calculated insulin sensitivity index was found after IAGTT between breeds. A time response (P < 0.05) was obtained for insulin, glucose and lactate so that maximum concentration was achieved at 10 and 15 min post-infusion for insulin (Iberian and Landrace pigs, respectively), immediately post-infusion for glucose, and 20 min post-infusion for lactate, decreasing thereafter until basal levels. There was no time effect for the rest of metabolites evaluated. In conclusion, growing Iberian pigs challenged with an IAGTT showed changes in biochemical parameters and insulin response that may indicate an early stage of insulin resistance. PeerJ Inc. 2021-03-10 /pmc/articles/PMC7955676/ /pubmed/33854837 http://dx.doi.org/10.7717/peerj.11014 Text en ©2021 Rodríguez-López et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Rodríguez-López, José Miguel
Lachica, Manuel
González-Valero, Lucrecia
Fernández-Fígares, Ignacio
Determining insulin sensitivity from glucose tolerance tests in Iberian and landrace pigs
title Determining insulin sensitivity from glucose tolerance tests in Iberian and landrace pigs
title_full Determining insulin sensitivity from glucose tolerance tests in Iberian and landrace pigs
title_fullStr Determining insulin sensitivity from glucose tolerance tests in Iberian and landrace pigs
title_full_unstemmed Determining insulin sensitivity from glucose tolerance tests in Iberian and landrace pigs
title_short Determining insulin sensitivity from glucose tolerance tests in Iberian and landrace pigs
title_sort determining insulin sensitivity from glucose tolerance tests in iberian and landrace pigs
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955676/
https://www.ncbi.nlm.nih.gov/pubmed/33854837
http://dx.doi.org/10.7717/peerj.11014
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