Cargando…

Rice NIN‐LIKE PROTEIN 4 plays a pivotal role in nitrogen use efficiency

Nitrogen (N) is one of the key essential macronutrients that affects rice growth and yield. Inorganic N fertilizers are excessively used to boost yield and generate serious collateral environmental pollution. Therefore, improving crop N use efficiency (NUE) is highly desirable and has been a major e...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Jie, Zhang, Zi‐Sheng, Xia, Jin‐Qiu, Alfatih, Alamin, Song, Ying, Huang, Yi‐Jie, Wan, Guang‐Yu, Sun, Liang‐Qi, Tang, Hui, Liu, Yang, Wang, Shi‐Mei, Zhu, Qi‐Sheng, Qin, Peng, Wang, Yu‐Ping, Li, Shi‐Gui, Mao, Chuan‐Zao, Zhang, Gui‐Quan, Chu, Chengcai, Yu, Lin‐Hui, Xiang, Cheng‐Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955889/
https://www.ncbi.nlm.nih.gov/pubmed/32876985
http://dx.doi.org/10.1111/pbi.13475
_version_ 1783664335614115840
author Wu, Jie
Zhang, Zi‐Sheng
Xia, Jin‐Qiu
Alfatih, Alamin
Song, Ying
Huang, Yi‐Jie
Wan, Guang‐Yu
Sun, Liang‐Qi
Tang, Hui
Liu, Yang
Wang, Shi‐Mei
Zhu, Qi‐Sheng
Qin, Peng
Wang, Yu‐Ping
Li, Shi‐Gui
Mao, Chuan‐Zao
Zhang, Gui‐Quan
Chu, Chengcai
Yu, Lin‐Hui
Xiang, Cheng‐Bin
author_facet Wu, Jie
Zhang, Zi‐Sheng
Xia, Jin‐Qiu
Alfatih, Alamin
Song, Ying
Huang, Yi‐Jie
Wan, Guang‐Yu
Sun, Liang‐Qi
Tang, Hui
Liu, Yang
Wang, Shi‐Mei
Zhu, Qi‐Sheng
Qin, Peng
Wang, Yu‐Ping
Li, Shi‐Gui
Mao, Chuan‐Zao
Zhang, Gui‐Quan
Chu, Chengcai
Yu, Lin‐Hui
Xiang, Cheng‐Bin
author_sort Wu, Jie
collection PubMed
description Nitrogen (N) is one of the key essential macronutrients that affects rice growth and yield. Inorganic N fertilizers are excessively used to boost yield and generate serious collateral environmental pollution. Therefore, improving crop N use efficiency (NUE) is highly desirable and has been a major endeavour in crop improvement. However, only a few regulators have been identified that can be used to improve NUE in rice to date. Here we show that the rice NIN‐like protein 4 (OsNLP4) significantly improves the rice NUE and yield. Field trials consistently showed that loss‐of‐OsNLP4 dramatically reduced yield and NUE compared with wild type under different N regimes. In contrast, the OsNLP4 overexpression lines remarkably increased yield by 30% and NUE by 47% under moderate N level compared with wild type. Transcriptomic analyses revealed that OsNLP4 orchestrates the expression of a majority of known N uptake, assimilation and signalling genes by directly binding to the nitrate‐responsive cis‐element in their promoters to regulate their expression. Moreover, overexpression of OsNLP4 can recover the phenotype of Arabidopsis nlp7 mutant and enhance its biomass. Our results demonstrate that OsNLP4 plays a pivotal role in rice NUE and sheds light on crop NUE improvement.
format Online
Article
Text
id pubmed-7955889
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-79558892021-03-18 Rice NIN‐LIKE PROTEIN 4 plays a pivotal role in nitrogen use efficiency Wu, Jie Zhang, Zi‐Sheng Xia, Jin‐Qiu Alfatih, Alamin Song, Ying Huang, Yi‐Jie Wan, Guang‐Yu Sun, Liang‐Qi Tang, Hui Liu, Yang Wang, Shi‐Mei Zhu, Qi‐Sheng Qin, Peng Wang, Yu‐Ping Li, Shi‐Gui Mao, Chuan‐Zao Zhang, Gui‐Quan Chu, Chengcai Yu, Lin‐Hui Xiang, Cheng‐Bin Plant Biotechnol J Research Articles Nitrogen (N) is one of the key essential macronutrients that affects rice growth and yield. Inorganic N fertilizers are excessively used to boost yield and generate serious collateral environmental pollution. Therefore, improving crop N use efficiency (NUE) is highly desirable and has been a major endeavour in crop improvement. However, only a few regulators have been identified that can be used to improve NUE in rice to date. Here we show that the rice NIN‐like protein 4 (OsNLP4) significantly improves the rice NUE and yield. Field trials consistently showed that loss‐of‐OsNLP4 dramatically reduced yield and NUE compared with wild type under different N regimes. In contrast, the OsNLP4 overexpression lines remarkably increased yield by 30% and NUE by 47% under moderate N level compared with wild type. Transcriptomic analyses revealed that OsNLP4 orchestrates the expression of a majority of known N uptake, assimilation and signalling genes by directly binding to the nitrate‐responsive cis‐element in their promoters to regulate their expression. Moreover, overexpression of OsNLP4 can recover the phenotype of Arabidopsis nlp7 mutant and enhance its biomass. Our results demonstrate that OsNLP4 plays a pivotal role in rice NUE and sheds light on crop NUE improvement. John Wiley and Sons Inc. 2020-09-17 2021-03 /pmc/articles/PMC7955889/ /pubmed/32876985 http://dx.doi.org/10.1111/pbi.13475 Text en © 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Wu, Jie
Zhang, Zi‐Sheng
Xia, Jin‐Qiu
Alfatih, Alamin
Song, Ying
Huang, Yi‐Jie
Wan, Guang‐Yu
Sun, Liang‐Qi
Tang, Hui
Liu, Yang
Wang, Shi‐Mei
Zhu, Qi‐Sheng
Qin, Peng
Wang, Yu‐Ping
Li, Shi‐Gui
Mao, Chuan‐Zao
Zhang, Gui‐Quan
Chu, Chengcai
Yu, Lin‐Hui
Xiang, Cheng‐Bin
Rice NIN‐LIKE PROTEIN 4 plays a pivotal role in nitrogen use efficiency
title Rice NIN‐LIKE PROTEIN 4 plays a pivotal role in nitrogen use efficiency
title_full Rice NIN‐LIKE PROTEIN 4 plays a pivotal role in nitrogen use efficiency
title_fullStr Rice NIN‐LIKE PROTEIN 4 plays a pivotal role in nitrogen use efficiency
title_full_unstemmed Rice NIN‐LIKE PROTEIN 4 plays a pivotal role in nitrogen use efficiency
title_short Rice NIN‐LIKE PROTEIN 4 plays a pivotal role in nitrogen use efficiency
title_sort rice nin‐like protein 4 plays a pivotal role in nitrogen use efficiency
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955889/
https://www.ncbi.nlm.nih.gov/pubmed/32876985
http://dx.doi.org/10.1111/pbi.13475
work_keys_str_mv AT wujie riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT zhangzisheng riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT xiajinqiu riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT alfatihalamin riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT songying riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT huangyijie riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT wanguangyu riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT sunliangqi riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT tanghui riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT liuyang riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT wangshimei riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT zhuqisheng riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT qinpeng riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT wangyuping riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT lishigui riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT maochuanzao riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT zhangguiquan riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT chuchengcai riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT yulinhui riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency
AT xiangchengbin riceninlikeprotein4playsapivotalroleinnitrogenuseefficiency