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Long-term persistence of RBD(+) memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection

Considerable concerns relating to the duration of protective immunity against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) exist, with evidence of antibody titers declining rapidly after infection and reports of reinfection. Here, we monitor the antibody responses against SARS-CoV-2...

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Autores principales: Abayasingam, Arunasingam, Balachandran, Harikrishnan, Agapiou, David, Hammoud, Mohamed, Rodrigo, Chaturaka, Keoshkerian, Elizabeth, Li, Hui, Brasher, Nicholas A., Christ, Daniel, Rouet, Romain, Burnet, Deborah, Grubor-Bauk, Branka, Rawlinson, William, Turville, Stuart, Aggarwal, Anupriya, Stella, Alberto Ospina, Fichter, Christina, Brilot, Fabienne, Mina, Michael, Post, Jeffrey J., Hudson, Bernard, Gilroy, Nicky, Dwyer, Dominic, Sasson, Sarah C., Tea, Fiona, Pilli, Deepti, Kelleher, Anthony, Tedla, Nicodemus, Lloyd, Andrew R., Martinello, Marianne, Bull, Rowena A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955929/
https://www.ncbi.nlm.nih.gov/pubmed/33748788
http://dx.doi.org/10.1016/j.xcrm.2021.100228
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author Abayasingam, Arunasingam
Balachandran, Harikrishnan
Agapiou, David
Hammoud, Mohamed
Rodrigo, Chaturaka
Keoshkerian, Elizabeth
Li, Hui
Brasher, Nicholas A.
Christ, Daniel
Rouet, Romain
Burnet, Deborah
Grubor-Bauk, Branka
Rawlinson, William
Turville, Stuart
Aggarwal, Anupriya
Stella, Alberto Ospina
Fichter, Christina
Brilot, Fabienne
Mina, Michael
Post, Jeffrey J.
Hudson, Bernard
Gilroy, Nicky
Dwyer, Dominic
Sasson, Sarah C.
Tea, Fiona
Pilli, Deepti
Kelleher, Anthony
Tedla, Nicodemus
Lloyd, Andrew R.
Martinello, Marianne
Bull, Rowena A.
author_facet Abayasingam, Arunasingam
Balachandran, Harikrishnan
Agapiou, David
Hammoud, Mohamed
Rodrigo, Chaturaka
Keoshkerian, Elizabeth
Li, Hui
Brasher, Nicholas A.
Christ, Daniel
Rouet, Romain
Burnet, Deborah
Grubor-Bauk, Branka
Rawlinson, William
Turville, Stuart
Aggarwal, Anupriya
Stella, Alberto Ospina
Fichter, Christina
Brilot, Fabienne
Mina, Michael
Post, Jeffrey J.
Hudson, Bernard
Gilroy, Nicky
Dwyer, Dominic
Sasson, Sarah C.
Tea, Fiona
Pilli, Deepti
Kelleher, Anthony
Tedla, Nicodemus
Lloyd, Andrew R.
Martinello, Marianne
Bull, Rowena A.
author_sort Abayasingam, Arunasingam
collection PubMed
description Considerable concerns relating to the duration of protective immunity against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) exist, with evidence of antibody titers declining rapidly after infection and reports of reinfection. Here, we monitor the antibody responses against SARS-CoV-2 receptor-binding domain (RBD) for up to 6 months after infection. While antibody titers are maintained, ∼13% of the cohort’s neutralizing responses return to background. However, encouragingly, in a selected subset of 13 participants, 12 have detectable RBD-specific memory B cells and these generally are increasing out to 6 months. Furthermore, we are able to generate monoclonal antibodies with SARS-CoV-2 neutralizing capacity from these memory B cells. Overall, our study suggests that the loss of neutralizing antibodies in plasma may be countered by the maintenance of neutralizing capacity in the memory B cell repertoire.
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spelling pubmed-79559292021-03-15 Long-term persistence of RBD(+) memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection Abayasingam, Arunasingam Balachandran, Harikrishnan Agapiou, David Hammoud, Mohamed Rodrigo, Chaturaka Keoshkerian, Elizabeth Li, Hui Brasher, Nicholas A. Christ, Daniel Rouet, Romain Burnet, Deborah Grubor-Bauk, Branka Rawlinson, William Turville, Stuart Aggarwal, Anupriya Stella, Alberto Ospina Fichter, Christina Brilot, Fabienne Mina, Michael Post, Jeffrey J. Hudson, Bernard Gilroy, Nicky Dwyer, Dominic Sasson, Sarah C. Tea, Fiona Pilli, Deepti Kelleher, Anthony Tedla, Nicodemus Lloyd, Andrew R. Martinello, Marianne Bull, Rowena A. Cell Rep Med Report Considerable concerns relating to the duration of protective immunity against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) exist, with evidence of antibody titers declining rapidly after infection and reports of reinfection. Here, we monitor the antibody responses against SARS-CoV-2 receptor-binding domain (RBD) for up to 6 months after infection. While antibody titers are maintained, ∼13% of the cohort’s neutralizing responses return to background. However, encouragingly, in a selected subset of 13 participants, 12 have detectable RBD-specific memory B cells and these generally are increasing out to 6 months. Furthermore, we are able to generate monoclonal antibodies with SARS-CoV-2 neutralizing capacity from these memory B cells. Overall, our study suggests that the loss of neutralizing antibodies in plasma may be countered by the maintenance of neutralizing capacity in the memory B cell repertoire. Elsevier 2021-03-14 /pmc/articles/PMC7955929/ /pubmed/33748788 http://dx.doi.org/10.1016/j.xcrm.2021.100228 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Report
Abayasingam, Arunasingam
Balachandran, Harikrishnan
Agapiou, David
Hammoud, Mohamed
Rodrigo, Chaturaka
Keoshkerian, Elizabeth
Li, Hui
Brasher, Nicholas A.
Christ, Daniel
Rouet, Romain
Burnet, Deborah
Grubor-Bauk, Branka
Rawlinson, William
Turville, Stuart
Aggarwal, Anupriya
Stella, Alberto Ospina
Fichter, Christina
Brilot, Fabienne
Mina, Michael
Post, Jeffrey J.
Hudson, Bernard
Gilroy, Nicky
Dwyer, Dominic
Sasson, Sarah C.
Tea, Fiona
Pilli, Deepti
Kelleher, Anthony
Tedla, Nicodemus
Lloyd, Andrew R.
Martinello, Marianne
Bull, Rowena A.
Long-term persistence of RBD(+) memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection
title Long-term persistence of RBD(+) memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection
title_full Long-term persistence of RBD(+) memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection
title_fullStr Long-term persistence of RBD(+) memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection
title_full_unstemmed Long-term persistence of RBD(+) memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection
title_short Long-term persistence of RBD(+) memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection
title_sort long-term persistence of rbd(+) memory b cells encoding neutralizing antibodies in sars-cov-2 infection
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955929/
https://www.ncbi.nlm.nih.gov/pubmed/33748788
http://dx.doi.org/10.1016/j.xcrm.2021.100228
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