Cargando…
Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice
Photoreceptors are the light-sensing cells of the retina and the major cell type affected in most inherited retinal degenerations. Different metabolic pathways sustain their high energetic demand in physiological conditions, particularly aerobic glycolysis. The principal metabolome of the mature ret...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956175/ https://www.ncbi.nlm.nih.gov/pubmed/33652907 http://dx.doi.org/10.3390/ijms22052345 |
_version_ | 1783664378424328192 |
---|---|
author | Murenu, Elisa Kostidis, Sarantos Lahiri, Shibojyoti Geserich, Anna S. Imhof, Axel Giera, Martin Michalakis, Stylianos |
author_facet | Murenu, Elisa Kostidis, Sarantos Lahiri, Shibojyoti Geserich, Anna S. Imhof, Axel Giera, Martin Michalakis, Stylianos |
author_sort | Murenu, Elisa |
collection | PubMed |
description | Photoreceptors are the light-sensing cells of the retina and the major cell type affected in most inherited retinal degenerations. Different metabolic pathways sustain their high energetic demand in physiological conditions, particularly aerobic glycolysis. The principal metabolome of the mature retina has been studied, but only limited information is available on metabolic adaptations in response to key developmental events, such as eye opening. Moreover, dynamic metabolic changes due to retinal degeneration are not well understood. Here, we aimed to explore and map the ocular metabolic dynamics induced by eye opening in healthy (wild type) or Pde6b-mutant (retinal degeneration 1, Rd1) mice, in which photoreceptors degenerate shortly after eye opening. To unravel metabolic differences emerging before and after eye opening under physiological and pathophysiological conditions, we performed nuclear magnetic resonance (NMR) spectrosco-py-based metabolome analysis of wild type and Rd1 retina and vitreous/lens. We show that eye opening is accompanied by changes in the concentration of selected metabolites in the retina and by alterations in the vitreous/lens composition only in the retinal degeneration context. As such, we identify N-Acetylaspartate as a potential novel vitreous/lens marker reflecting progressive retinal degeneration. Thus, our data can help elucidating mechanisms underlying key events in retinal physiology and reveal changes occurring in pathology, while highlighting the importance of the vitreous/lens in the characterization of retinal diseases. |
format | Online Article Text |
id | pubmed-7956175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79561752021-03-15 Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice Murenu, Elisa Kostidis, Sarantos Lahiri, Shibojyoti Geserich, Anna S. Imhof, Axel Giera, Martin Michalakis, Stylianos Int J Mol Sci Article Photoreceptors are the light-sensing cells of the retina and the major cell type affected in most inherited retinal degenerations. Different metabolic pathways sustain their high energetic demand in physiological conditions, particularly aerobic glycolysis. The principal metabolome of the mature retina has been studied, but only limited information is available on metabolic adaptations in response to key developmental events, such as eye opening. Moreover, dynamic metabolic changes due to retinal degeneration are not well understood. Here, we aimed to explore and map the ocular metabolic dynamics induced by eye opening in healthy (wild type) or Pde6b-mutant (retinal degeneration 1, Rd1) mice, in which photoreceptors degenerate shortly after eye opening. To unravel metabolic differences emerging before and after eye opening under physiological and pathophysiological conditions, we performed nuclear magnetic resonance (NMR) spectrosco-py-based metabolome analysis of wild type and Rd1 retina and vitreous/lens. We show that eye opening is accompanied by changes in the concentration of selected metabolites in the retina and by alterations in the vitreous/lens composition only in the retinal degeneration context. As such, we identify N-Acetylaspartate as a potential novel vitreous/lens marker reflecting progressive retinal degeneration. Thus, our data can help elucidating mechanisms underlying key events in retinal physiology and reveal changes occurring in pathology, while highlighting the importance of the vitreous/lens in the characterization of retinal diseases. MDPI 2021-02-26 /pmc/articles/PMC7956175/ /pubmed/33652907 http://dx.doi.org/10.3390/ijms22052345 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Murenu, Elisa Kostidis, Sarantos Lahiri, Shibojyoti Geserich, Anna S. Imhof, Axel Giera, Martin Michalakis, Stylianos Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice |
title | Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice |
title_full | Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice |
title_fullStr | Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice |
title_full_unstemmed | Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice |
title_short | Metabolic Analysis of Vitreous/Lens and Retina in Wild Type and Retinal Degeneration Mice |
title_sort | metabolic analysis of vitreous/lens and retina in wild type and retinal degeneration mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956175/ https://www.ncbi.nlm.nih.gov/pubmed/33652907 http://dx.doi.org/10.3390/ijms22052345 |
work_keys_str_mv | AT murenuelisa metabolicanalysisofvitreouslensandretinainwildtypeandretinaldegenerationmice AT kostidissarantos metabolicanalysisofvitreouslensandretinainwildtypeandretinaldegenerationmice AT lahirishibojyoti metabolicanalysisofvitreouslensandretinainwildtypeandretinaldegenerationmice AT geserichannas metabolicanalysisofvitreouslensandretinainwildtypeandretinaldegenerationmice AT imhofaxel metabolicanalysisofvitreouslensandretinainwildtypeandretinaldegenerationmice AT gieramartin metabolicanalysisofvitreouslensandretinainwildtypeandretinaldegenerationmice AT michalakisstylianos metabolicanalysisofvitreouslensandretinainwildtypeandretinaldegenerationmice |