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Does a Red House Affect Rhythms in Mice with a Corrupted Circadian System?

The circadian rhythms of body functions in mammals are controlled by the circadian system. The suprachiasmatic nucleus (SCN) in the hypothalamus orchestrates subordinate oscillators. Time information is conveyed from the retina to the SCN to coordinate an organism’s physiology and behavior with the...

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Autores principales: Öztürk, Menekse, Ingenwerth, Marc, Sager, Martin, von Gall, Charlotte, Ali, Amira A. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956239/
https://www.ncbi.nlm.nih.gov/pubmed/33669004
http://dx.doi.org/10.3390/ijms22052288
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author Öztürk, Menekse
Ingenwerth, Marc
Sager, Martin
von Gall, Charlotte
Ali, Amira A. H.
author_facet Öztürk, Menekse
Ingenwerth, Marc
Sager, Martin
von Gall, Charlotte
Ali, Amira A. H.
author_sort Öztürk, Menekse
collection PubMed
description The circadian rhythms of body functions in mammals are controlled by the circadian system. The suprachiasmatic nucleus (SCN) in the hypothalamus orchestrates subordinate oscillators. Time information is conveyed from the retina to the SCN to coordinate an organism’s physiology and behavior with the light/dark cycle. At the cellular level, molecular clockwork composed of interlocked transcriptional/translational feedback loops of clock genes drives rhythmic gene expression. Mice with targeted deletion of the essential clock gene Bmal1 (Bmal1−/−) have an impaired light input pathway into the circadian system and show a loss of circadian rhythms. The red house (RH) is an animal welfare measure widely used for rodents as a hiding place. Red plastic provides light at a low irradiance and long wavelength—conditions which affect the circadian system. It is not known yet whether the RH affects rhythmic behavior in mice with a corrupted circadian system. Here, we analyzed whether the RH affects spontaneous locomotor activity in Bmal1−/− mice under standard laboratory light conditions. In addition, mPER1- and p-ERK-immunoreactions, as markers for rhythmic SCN neuronal activity, and day/night plasma corticosterone levels were evaluated. Our findings indicate that application of the RH to Bmal1−/− abolishes rhythmic locomotor behavior and dampens rhythmic SCN neuronal activity. However, RH had no effect on the day/night difference in corticosterone levels.
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spelling pubmed-79562392021-03-15 Does a Red House Affect Rhythms in Mice with a Corrupted Circadian System? Öztürk, Menekse Ingenwerth, Marc Sager, Martin von Gall, Charlotte Ali, Amira A. H. Int J Mol Sci Article The circadian rhythms of body functions in mammals are controlled by the circadian system. The suprachiasmatic nucleus (SCN) in the hypothalamus orchestrates subordinate oscillators. Time information is conveyed from the retina to the SCN to coordinate an organism’s physiology and behavior with the light/dark cycle. At the cellular level, molecular clockwork composed of interlocked transcriptional/translational feedback loops of clock genes drives rhythmic gene expression. Mice with targeted deletion of the essential clock gene Bmal1 (Bmal1−/−) have an impaired light input pathway into the circadian system and show a loss of circadian rhythms. The red house (RH) is an animal welfare measure widely used for rodents as a hiding place. Red plastic provides light at a low irradiance and long wavelength—conditions which affect the circadian system. It is not known yet whether the RH affects rhythmic behavior in mice with a corrupted circadian system. Here, we analyzed whether the RH affects spontaneous locomotor activity in Bmal1−/− mice under standard laboratory light conditions. In addition, mPER1- and p-ERK-immunoreactions, as markers for rhythmic SCN neuronal activity, and day/night plasma corticosterone levels were evaluated. Our findings indicate that application of the RH to Bmal1−/− abolishes rhythmic locomotor behavior and dampens rhythmic SCN neuronal activity. However, RH had no effect on the day/night difference in corticosterone levels. MDPI 2021-02-25 /pmc/articles/PMC7956239/ /pubmed/33669004 http://dx.doi.org/10.3390/ijms22052288 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Öztürk, Menekse
Ingenwerth, Marc
Sager, Martin
von Gall, Charlotte
Ali, Amira A. H.
Does a Red House Affect Rhythms in Mice with a Corrupted Circadian System?
title Does a Red House Affect Rhythms in Mice with a Corrupted Circadian System?
title_full Does a Red House Affect Rhythms in Mice with a Corrupted Circadian System?
title_fullStr Does a Red House Affect Rhythms in Mice with a Corrupted Circadian System?
title_full_unstemmed Does a Red House Affect Rhythms in Mice with a Corrupted Circadian System?
title_short Does a Red House Affect Rhythms in Mice with a Corrupted Circadian System?
title_sort does a red house affect rhythms in mice with a corrupted circadian system?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956239/
https://www.ncbi.nlm.nih.gov/pubmed/33669004
http://dx.doi.org/10.3390/ijms22052288
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