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Mechanical Characterization of Human Trabecular and Formed Granulate Bone Cylinders Processed by High Hydrostatic Pressure

One main disadvantage of commercially available allogenic bone substitute materials is the altered mechanical behavior due to applied material processing, including sterilization methods like thermal processing or gamma irradiation. The use of high hydrostatic pressure (HHP) might be a gentle altern...

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Autores principales: Waletzko-Hellwig, Janine, Saemann, Michael, Schulze, Marko, Frerich, Bernhard, Bader, Rainer, Dau, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956279/
https://www.ncbi.nlm.nih.gov/pubmed/33668996
http://dx.doi.org/10.3390/ma14051069
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author Waletzko-Hellwig, Janine
Saemann, Michael
Schulze, Marko
Frerich, Bernhard
Bader, Rainer
Dau, Michael
author_facet Waletzko-Hellwig, Janine
Saemann, Michael
Schulze, Marko
Frerich, Bernhard
Bader, Rainer
Dau, Michael
author_sort Waletzko-Hellwig, Janine
collection PubMed
description One main disadvantage of commercially available allogenic bone substitute materials is the altered mechanical behavior due to applied material processing, including sterilization methods like thermal processing or gamma irradiation. The use of high hydrostatic pressure (HHP) might be a gentle alternative to avoid mechanical alteration. Therefore, we compressed ground trabecular human bone to granules and, afterwards, treated them with 250 and 300 MPa for 20 and 30 min respectively. We characterized the formed bone granule cylinders (BGC) with respect to their biomechanical properties by evaluating stiffness and stress at 15% strain. Furthermore, the stiffness and yield strength of HHP-treated and native human trabecular bone cylinders (TBC) as control were evaluated. The mechanical properties of native vs. HHP-treated TBCs as well as HHP-treated vs. untreated BGCs did not differ, independent of the applied HHP magnitude and duration. Our study suggests HHP treatment as a suitable alternative to current processing techniques for allogenic bone substitutes since no negative effects on mechanical properties occurred.
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spelling pubmed-79562792021-03-15 Mechanical Characterization of Human Trabecular and Formed Granulate Bone Cylinders Processed by High Hydrostatic Pressure Waletzko-Hellwig, Janine Saemann, Michael Schulze, Marko Frerich, Bernhard Bader, Rainer Dau, Michael Materials (Basel) Article One main disadvantage of commercially available allogenic bone substitute materials is the altered mechanical behavior due to applied material processing, including sterilization methods like thermal processing or gamma irradiation. The use of high hydrostatic pressure (HHP) might be a gentle alternative to avoid mechanical alteration. Therefore, we compressed ground trabecular human bone to granules and, afterwards, treated them with 250 and 300 MPa for 20 and 30 min respectively. We characterized the formed bone granule cylinders (BGC) with respect to their biomechanical properties by evaluating stiffness and stress at 15% strain. Furthermore, the stiffness and yield strength of HHP-treated and native human trabecular bone cylinders (TBC) as control were evaluated. The mechanical properties of native vs. HHP-treated TBCs as well as HHP-treated vs. untreated BGCs did not differ, independent of the applied HHP magnitude and duration. Our study suggests HHP treatment as a suitable alternative to current processing techniques for allogenic bone substitutes since no negative effects on mechanical properties occurred. MDPI 2021-02-25 /pmc/articles/PMC7956279/ /pubmed/33668996 http://dx.doi.org/10.3390/ma14051069 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Waletzko-Hellwig, Janine
Saemann, Michael
Schulze, Marko
Frerich, Bernhard
Bader, Rainer
Dau, Michael
Mechanical Characterization of Human Trabecular and Formed Granulate Bone Cylinders Processed by High Hydrostatic Pressure
title Mechanical Characterization of Human Trabecular and Formed Granulate Bone Cylinders Processed by High Hydrostatic Pressure
title_full Mechanical Characterization of Human Trabecular and Formed Granulate Bone Cylinders Processed by High Hydrostatic Pressure
title_fullStr Mechanical Characterization of Human Trabecular and Formed Granulate Bone Cylinders Processed by High Hydrostatic Pressure
title_full_unstemmed Mechanical Characterization of Human Trabecular and Formed Granulate Bone Cylinders Processed by High Hydrostatic Pressure
title_short Mechanical Characterization of Human Trabecular and Formed Granulate Bone Cylinders Processed by High Hydrostatic Pressure
title_sort mechanical characterization of human trabecular and formed granulate bone cylinders processed by high hydrostatic pressure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956279/
https://www.ncbi.nlm.nih.gov/pubmed/33668996
http://dx.doi.org/10.3390/ma14051069
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