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Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet
This research prepared chitosan–PLA plastic films by extrusion, analyzed the physical and mechanical properties and antibacterial activity of the fabricated plastic films, and used them to preserve grouper fillet. We added chitosan (220 kDa, 93% DD) in the weight ratio of 0.5–2% into the PLA to prep...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956300/ https://www.ncbi.nlm.nih.gov/pubmed/33669080 http://dx.doi.org/10.3390/polym13050696 |
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author | Chang, Shun-Hsien Chen, Ying-Ju Tseng, Hsiang-Jung Hsiao, Hsin-I Chai, Huey-Jine Shang, Kuo-Chung Pan, Chorng-Liang Tsai, Guo-Jane |
author_facet | Chang, Shun-Hsien Chen, Ying-Ju Tseng, Hsiang-Jung Hsiao, Hsin-I Chai, Huey-Jine Shang, Kuo-Chung Pan, Chorng-Liang Tsai, Guo-Jane |
author_sort | Chang, Shun-Hsien |
collection | PubMed |
description | This research prepared chitosan–PLA plastic films by extrusion, analyzed the physical and mechanical properties and antibacterial activity of the fabricated plastic films, and used them to preserve grouper fillet. We added chitosan (220 kDa, 93% DD) in the weight ratio of 0.5–2% into the PLA to prepare the chitosan–PLA films. With the increasing chitosan dosage, both the water vapor transmission rate and moisture content of chitosan–PLA films increased. Among the three doses of chitosan (0.5%, 1%, and 2%) added to PLA, 0.5% chitosan–PLA film had the highest antibacterial activity. This plastic film had an inhibitory efficiency of over 95% against Escherichia coli, Pseudomonas fluorescens, and Staphylococcus aureus. The action of covering the fish fillet with 0.5% chitosan–PLA film significantly reduced several microbes’ counting (i.e., mesophiles, psychrophiles, coliforms, Pseudomonas, Aeromonas, and Vibrio) and total volatile basic nitrogen (TVBN) value in the grouper fillets stored at 4 °C. Thus, such action prolongs the fish fillets’ shelf life to up to at least nine days, and this 0.5% chitosan–PLA film shows promising potential for preserving refrigerated fish. |
format | Online Article Text |
id | pubmed-7956300 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79563002021-03-15 Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet Chang, Shun-Hsien Chen, Ying-Ju Tseng, Hsiang-Jung Hsiao, Hsin-I Chai, Huey-Jine Shang, Kuo-Chung Pan, Chorng-Liang Tsai, Guo-Jane Polymers (Basel) Article This research prepared chitosan–PLA plastic films by extrusion, analyzed the physical and mechanical properties and antibacterial activity of the fabricated plastic films, and used them to preserve grouper fillet. We added chitosan (220 kDa, 93% DD) in the weight ratio of 0.5–2% into the PLA to prepare the chitosan–PLA films. With the increasing chitosan dosage, both the water vapor transmission rate and moisture content of chitosan–PLA films increased. Among the three doses of chitosan (0.5%, 1%, and 2%) added to PLA, 0.5% chitosan–PLA film had the highest antibacterial activity. This plastic film had an inhibitory efficiency of over 95% against Escherichia coli, Pseudomonas fluorescens, and Staphylococcus aureus. The action of covering the fish fillet with 0.5% chitosan–PLA film significantly reduced several microbes’ counting (i.e., mesophiles, psychrophiles, coliforms, Pseudomonas, Aeromonas, and Vibrio) and total volatile basic nitrogen (TVBN) value in the grouper fillets stored at 4 °C. Thus, such action prolongs the fish fillets’ shelf life to up to at least nine days, and this 0.5% chitosan–PLA film shows promising potential for preserving refrigerated fish. MDPI 2021-02-25 /pmc/articles/PMC7956300/ /pubmed/33669080 http://dx.doi.org/10.3390/polym13050696 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chang, Shun-Hsien Chen, Ying-Ju Tseng, Hsiang-Jung Hsiao, Hsin-I Chai, Huey-Jine Shang, Kuo-Chung Pan, Chorng-Liang Tsai, Guo-Jane Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet |
title | Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet |
title_full | Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet |
title_fullStr | Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet |
title_full_unstemmed | Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet |
title_short | Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet |
title_sort | antibacterial activity of chitosan–polylactate fabricated plastic film and its application on the preservation of fish fillet |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956300/ https://www.ncbi.nlm.nih.gov/pubmed/33669080 http://dx.doi.org/10.3390/polym13050696 |
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