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Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet

This research prepared chitosan–PLA plastic films by extrusion, analyzed the physical and mechanical properties and antibacterial activity of the fabricated plastic films, and used them to preserve grouper fillet. We added chitosan (220 kDa, 93% DD) in the weight ratio of 0.5–2% into the PLA to prep...

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Autores principales: Chang, Shun-Hsien, Chen, Ying-Ju, Tseng, Hsiang-Jung, Hsiao, Hsin-I, Chai, Huey-Jine, Shang, Kuo-Chung, Pan, Chorng-Liang, Tsai, Guo-Jane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956300/
https://www.ncbi.nlm.nih.gov/pubmed/33669080
http://dx.doi.org/10.3390/polym13050696
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author Chang, Shun-Hsien
Chen, Ying-Ju
Tseng, Hsiang-Jung
Hsiao, Hsin-I
Chai, Huey-Jine
Shang, Kuo-Chung
Pan, Chorng-Liang
Tsai, Guo-Jane
author_facet Chang, Shun-Hsien
Chen, Ying-Ju
Tseng, Hsiang-Jung
Hsiao, Hsin-I
Chai, Huey-Jine
Shang, Kuo-Chung
Pan, Chorng-Liang
Tsai, Guo-Jane
author_sort Chang, Shun-Hsien
collection PubMed
description This research prepared chitosan–PLA plastic films by extrusion, analyzed the physical and mechanical properties and antibacterial activity of the fabricated plastic films, and used them to preserve grouper fillet. We added chitosan (220 kDa, 93% DD) in the weight ratio of 0.5–2% into the PLA to prepare the chitosan–PLA films. With the increasing chitosan dosage, both the water vapor transmission rate and moisture content of chitosan–PLA films increased. Among the three doses of chitosan (0.5%, 1%, and 2%) added to PLA, 0.5% chitosan–PLA film had the highest antibacterial activity. This plastic film had an inhibitory efficiency of over 95% against Escherichia coli, Pseudomonas fluorescens, and Staphylococcus aureus. The action of covering the fish fillet with 0.5% chitosan–PLA film significantly reduced several microbes’ counting (i.e., mesophiles, psychrophiles, coliforms, Pseudomonas, Aeromonas, and Vibrio) and total volatile basic nitrogen (TVBN) value in the grouper fillets stored at 4 °C. Thus, such action prolongs the fish fillets’ shelf life to up to at least nine days, and this 0.5% chitosan–PLA film shows promising potential for preserving refrigerated fish.
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spelling pubmed-79563002021-03-15 Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet Chang, Shun-Hsien Chen, Ying-Ju Tseng, Hsiang-Jung Hsiao, Hsin-I Chai, Huey-Jine Shang, Kuo-Chung Pan, Chorng-Liang Tsai, Guo-Jane Polymers (Basel) Article This research prepared chitosan–PLA plastic films by extrusion, analyzed the physical and mechanical properties and antibacterial activity of the fabricated plastic films, and used them to preserve grouper fillet. We added chitosan (220 kDa, 93% DD) in the weight ratio of 0.5–2% into the PLA to prepare the chitosan–PLA films. With the increasing chitosan dosage, both the water vapor transmission rate and moisture content of chitosan–PLA films increased. Among the three doses of chitosan (0.5%, 1%, and 2%) added to PLA, 0.5% chitosan–PLA film had the highest antibacterial activity. This plastic film had an inhibitory efficiency of over 95% against Escherichia coli, Pseudomonas fluorescens, and Staphylococcus aureus. The action of covering the fish fillet with 0.5% chitosan–PLA film significantly reduced several microbes’ counting (i.e., mesophiles, psychrophiles, coliforms, Pseudomonas, Aeromonas, and Vibrio) and total volatile basic nitrogen (TVBN) value in the grouper fillets stored at 4 °C. Thus, such action prolongs the fish fillets’ shelf life to up to at least nine days, and this 0.5% chitosan–PLA film shows promising potential for preserving refrigerated fish. MDPI 2021-02-25 /pmc/articles/PMC7956300/ /pubmed/33669080 http://dx.doi.org/10.3390/polym13050696 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chang, Shun-Hsien
Chen, Ying-Ju
Tseng, Hsiang-Jung
Hsiao, Hsin-I
Chai, Huey-Jine
Shang, Kuo-Chung
Pan, Chorng-Liang
Tsai, Guo-Jane
Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet
title Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet
title_full Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet
title_fullStr Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet
title_full_unstemmed Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet
title_short Antibacterial Activity of Chitosan–Polylactate Fabricated Plastic Film and Its Application on the Preservation of Fish Fillet
title_sort antibacterial activity of chitosan–polylactate fabricated plastic film and its application on the preservation of fish fillet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956300/
https://www.ncbi.nlm.nih.gov/pubmed/33669080
http://dx.doi.org/10.3390/polym13050696
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