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The Cysteine-Rich Peptide Snakin-2 Negatively Regulates Tubers Sprouting through Modulating Lignin Biosynthesis and H(2)O(2) Accumulation in Potato
Potato tuber dormancy is critical for the post-harvest quality. Snakin/Gibberellic Acid Stimulated in Arabidopsis (GASA) family genes are involved in the plants’ defense against pathogens and in growth and development, but the effect of Snakin-2 (SN2) on tuber dormancy and sprouting is largely unkno...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956376/ https://www.ncbi.nlm.nih.gov/pubmed/33669030 http://dx.doi.org/10.3390/ijms22052287 |
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author | Deng, Mengsheng Peng, Jie Zhang, Jie Ran, Shuang Cai, Chengcheng Yu, Liping Ni, Su Huang, Xueli Li, Liqin Wang, Xiyao |
author_facet | Deng, Mengsheng Peng, Jie Zhang, Jie Ran, Shuang Cai, Chengcheng Yu, Liping Ni, Su Huang, Xueli Li, Liqin Wang, Xiyao |
author_sort | Deng, Mengsheng |
collection | PubMed |
description | Potato tuber dormancy is critical for the post-harvest quality. Snakin/Gibberellic Acid Stimulated in Arabidopsis (GASA) family genes are involved in the plants’ defense against pathogens and in growth and development, but the effect of Snakin-2 (SN2) on tuber dormancy and sprouting is largely unknown. In this study, a transgenic approach was applied to manipulate the expression level of SN2 in tubers, and it demonstrated that StSN2 significantly controlled tuber sprouting, and silencing StSN2 resulted in a release of dormancy and overexpressing tubers showed a longer dormant period than that of the control. Further analyses revealed that the decrease expression level accelerated skin cracking and water loss. Metabolite analyses revealed that StSN2 significantly down-regulated the accumulation of lignin precursors in the periderm, and the change of lignin content was documented, a finding which was consistent with the precursors’ level. Subsequently, proteomics found that cinnamyl alcohol dehydrogenase (CAD), caffeic acid O-methyltransferase (COMT) and peroxidase (Prx), the key proteins for lignin synthesis, were significantly up-regulated in silencing lines, and gene expression and enzyme activity analyses also supported this effect. Interestingly, we found that StSN2 physically interacts with three peroxidases catalyzing the oxidation and polymerization of lignin. In addition, SN2 altered the hydrogen peroxide (H(2)O(2)) content and the activities of superoxide dismutase (SOD) and catalase (CAT). These results suggest that StSN2 negatively regulates lignin biosynthesis and H(2)O(2) accumulation, and ultimately inhibits the sprouting of potato tubers. |
format | Online Article Text |
id | pubmed-7956376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79563762021-03-16 The Cysteine-Rich Peptide Snakin-2 Negatively Regulates Tubers Sprouting through Modulating Lignin Biosynthesis and H(2)O(2) Accumulation in Potato Deng, Mengsheng Peng, Jie Zhang, Jie Ran, Shuang Cai, Chengcheng Yu, Liping Ni, Su Huang, Xueli Li, Liqin Wang, Xiyao Int J Mol Sci Article Potato tuber dormancy is critical for the post-harvest quality. Snakin/Gibberellic Acid Stimulated in Arabidopsis (GASA) family genes are involved in the plants’ defense against pathogens and in growth and development, but the effect of Snakin-2 (SN2) on tuber dormancy and sprouting is largely unknown. In this study, a transgenic approach was applied to manipulate the expression level of SN2 in tubers, and it demonstrated that StSN2 significantly controlled tuber sprouting, and silencing StSN2 resulted in a release of dormancy and overexpressing tubers showed a longer dormant period than that of the control. Further analyses revealed that the decrease expression level accelerated skin cracking and water loss. Metabolite analyses revealed that StSN2 significantly down-regulated the accumulation of lignin precursors in the periderm, and the change of lignin content was documented, a finding which was consistent with the precursors’ level. Subsequently, proteomics found that cinnamyl alcohol dehydrogenase (CAD), caffeic acid O-methyltransferase (COMT) and peroxidase (Prx), the key proteins for lignin synthesis, were significantly up-regulated in silencing lines, and gene expression and enzyme activity analyses also supported this effect. Interestingly, we found that StSN2 physically interacts with three peroxidases catalyzing the oxidation and polymerization of lignin. In addition, SN2 altered the hydrogen peroxide (H(2)O(2)) content and the activities of superoxide dismutase (SOD) and catalase (CAT). These results suggest that StSN2 negatively regulates lignin biosynthesis and H(2)O(2) accumulation, and ultimately inhibits the sprouting of potato tubers. MDPI 2021-02-25 /pmc/articles/PMC7956376/ /pubmed/33669030 http://dx.doi.org/10.3390/ijms22052287 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Deng, Mengsheng Peng, Jie Zhang, Jie Ran, Shuang Cai, Chengcheng Yu, Liping Ni, Su Huang, Xueli Li, Liqin Wang, Xiyao The Cysteine-Rich Peptide Snakin-2 Negatively Regulates Tubers Sprouting through Modulating Lignin Biosynthesis and H(2)O(2) Accumulation in Potato |
title | The Cysteine-Rich Peptide Snakin-2 Negatively Regulates Tubers Sprouting through Modulating Lignin Biosynthesis and H(2)O(2) Accumulation in Potato |
title_full | The Cysteine-Rich Peptide Snakin-2 Negatively Regulates Tubers Sprouting through Modulating Lignin Biosynthesis and H(2)O(2) Accumulation in Potato |
title_fullStr | The Cysteine-Rich Peptide Snakin-2 Negatively Regulates Tubers Sprouting through Modulating Lignin Biosynthesis and H(2)O(2) Accumulation in Potato |
title_full_unstemmed | The Cysteine-Rich Peptide Snakin-2 Negatively Regulates Tubers Sprouting through Modulating Lignin Biosynthesis and H(2)O(2) Accumulation in Potato |
title_short | The Cysteine-Rich Peptide Snakin-2 Negatively Regulates Tubers Sprouting through Modulating Lignin Biosynthesis and H(2)O(2) Accumulation in Potato |
title_sort | cysteine-rich peptide snakin-2 negatively regulates tubers sprouting through modulating lignin biosynthesis and h(2)o(2) accumulation in potato |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956376/ https://www.ncbi.nlm.nih.gov/pubmed/33669030 http://dx.doi.org/10.3390/ijms22052287 |
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