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Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans

Periodic fasting (PF) is an increasingly popular approach that assists in the management of metabolic and inflammatory diseases as well as in preventing mechanisms involved in aging. However, little is known about the effects of fasting on gut microbiota and its impact on the epigenetic regulation o...

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Autores principales: Lilja, Stephanie, Stoll, Carina, Krammer, Ulrike, Hippe, Berit, Duszka, Kalina, Debebe, Tewodros, Höfinger, Ingrid, König, Jürgen, Pointner, Angelika, Haslberger, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956384/
https://www.ncbi.nlm.nih.gov/pubmed/33652686
http://dx.doi.org/10.3390/ijms22052331
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author Lilja, Stephanie
Stoll, Carina
Krammer, Ulrike
Hippe, Berit
Duszka, Kalina
Debebe, Tewodros
Höfinger, Ingrid
König, Jürgen
Pointner, Angelika
Haslberger, Alexander
author_facet Lilja, Stephanie
Stoll, Carina
Krammer, Ulrike
Hippe, Berit
Duszka, Kalina
Debebe, Tewodros
Höfinger, Ingrid
König, Jürgen
Pointner, Angelika
Haslberger, Alexander
author_sort Lilja, Stephanie
collection PubMed
description Periodic fasting (PF) is an increasingly popular approach that assists in the management of metabolic and inflammatory diseases as well as in preventing mechanisms involved in aging. However, little is known about the effects of fasting on gut microbiota and its impact on the epigenetic regulation of metabolically relevant enzymes, especially sirtuins (SIRTs). We analyzed the effect of periodic fasting on the human gut microbiota, SIRTs expression, and mitochondrial content in 51 males and females. The participants fasted under supervision for five consecutive days following the Buchinger fasting guidelines. Ketogenesis, selected mRNAs, miRNAs, mitochondrial (mt) DNA, and gut composition were analyzed before and after PF. PF triggered a significant switch in metabolism, as indicated by the increase in ß-hydroxybutyrate (BHB) and pyruvate dehydrogenase kinase isoform 4 (PDK4) expression in the capillary blood. MtDNA, SIRT1, SIRT3, and miRlet7b-5p expression in blood cells were elevated, whereas SIRT6 and miR125b-5p were not affected. Following fasting, gut microbiota diversity increased, and a statistically significant correlation between SIRT1 gene expression and the abundance of Prevotella and Lactobacillus was detected. The abundance of longevity related Christensenella species increased after fasting and inversely correlated with age as well as body mass index (BMI). Thus, this represents the first study that showing that fasting not only changes the composition of the gut microbiota, making it more diverse, but also affects SIRT expression in humans.
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spelling pubmed-79563842021-03-16 Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans Lilja, Stephanie Stoll, Carina Krammer, Ulrike Hippe, Berit Duszka, Kalina Debebe, Tewodros Höfinger, Ingrid König, Jürgen Pointner, Angelika Haslberger, Alexander Int J Mol Sci Article Periodic fasting (PF) is an increasingly popular approach that assists in the management of metabolic and inflammatory diseases as well as in preventing mechanisms involved in aging. However, little is known about the effects of fasting on gut microbiota and its impact on the epigenetic regulation of metabolically relevant enzymes, especially sirtuins (SIRTs). We analyzed the effect of periodic fasting on the human gut microbiota, SIRTs expression, and mitochondrial content in 51 males and females. The participants fasted under supervision for five consecutive days following the Buchinger fasting guidelines. Ketogenesis, selected mRNAs, miRNAs, mitochondrial (mt) DNA, and gut composition were analyzed before and after PF. PF triggered a significant switch in metabolism, as indicated by the increase in ß-hydroxybutyrate (BHB) and pyruvate dehydrogenase kinase isoform 4 (PDK4) expression in the capillary blood. MtDNA, SIRT1, SIRT3, and miRlet7b-5p expression in blood cells were elevated, whereas SIRT6 and miR125b-5p were not affected. Following fasting, gut microbiota diversity increased, and a statistically significant correlation between SIRT1 gene expression and the abundance of Prevotella and Lactobacillus was detected. The abundance of longevity related Christensenella species increased after fasting and inversely correlated with age as well as body mass index (BMI). Thus, this represents the first study that showing that fasting not only changes the composition of the gut microbiota, making it more diverse, but also affects SIRT expression in humans. MDPI 2021-02-26 /pmc/articles/PMC7956384/ /pubmed/33652686 http://dx.doi.org/10.3390/ijms22052331 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lilja, Stephanie
Stoll, Carina
Krammer, Ulrike
Hippe, Berit
Duszka, Kalina
Debebe, Tewodros
Höfinger, Ingrid
König, Jürgen
Pointner, Angelika
Haslberger, Alexander
Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans
title Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans
title_full Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans
title_fullStr Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans
title_full_unstemmed Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans
title_short Five Days Periodic Fasting Elevates Levels of Longevity Related Christensenella and Sirtuin Expression in Humans
title_sort five days periodic fasting elevates levels of longevity related christensenella and sirtuin expression in humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956384/
https://www.ncbi.nlm.nih.gov/pubmed/33652686
http://dx.doi.org/10.3390/ijms22052331
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