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The Effects of Naringenin on miRNA-mRNA Profiles in HepaRG Cells

Naringenin, a natural flavonoid widely found in citrus fruits, has been reported to possess anti-oxidant, anti-inflammatory, and hepatoprotective properties as a natural dietary supplement. However, the regulatory mechanism of naringenin in human liver remains unclear. In the present study, messenge...

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Detalles Bibliográficos
Autores principales: Fan, Weiyang, Shi, Rui, Guan, Minyi, Chen, Pan, Wu, Hao, Su, Weiwei, Wang, Yonggang, Li, Peibo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956767/
https://www.ncbi.nlm.nih.gov/pubmed/33669020
http://dx.doi.org/10.3390/ijms22052292
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author Fan, Weiyang
Shi, Rui
Guan, Minyi
Chen, Pan
Wu, Hao
Su, Weiwei
Wang, Yonggang
Li, Peibo
author_facet Fan, Weiyang
Shi, Rui
Guan, Minyi
Chen, Pan
Wu, Hao
Su, Weiwei
Wang, Yonggang
Li, Peibo
author_sort Fan, Weiyang
collection PubMed
description Naringenin, a natural flavonoid widely found in citrus fruits, has been reported to possess anti-oxidant, anti-inflammatory, and hepatoprotective properties as a natural dietary supplement. However, the regulatory mechanism of naringenin in human liver remains unclear. In the present study, messenger RNA sequencing (mRNA-seq), microRNA sequencing (miRNA-seq), and real-time qPCR were used to distinguish the expression differences between control and naringenin-treated HepaRG cells. We obtained 1037 differentially expressed mRNAs and 234 miRNAs. According to the target prediction and integration analysis in silico, we found 20 potential miRNA-mRNA pairs involved in liver metabolism. This study is the first to provide a perspective of miRNA–mRNA interactions in the regulation of naringenin via an integrated analysis of mRNA-seq and miRNA-seq in HepaRG cells, which further characterizes the nutraceutical value of naringenin as a food additive.
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spelling pubmed-79567672021-03-16 The Effects of Naringenin on miRNA-mRNA Profiles in HepaRG Cells Fan, Weiyang Shi, Rui Guan, Minyi Chen, Pan Wu, Hao Su, Weiwei Wang, Yonggang Li, Peibo Int J Mol Sci Article Naringenin, a natural flavonoid widely found in citrus fruits, has been reported to possess anti-oxidant, anti-inflammatory, and hepatoprotective properties as a natural dietary supplement. However, the regulatory mechanism of naringenin in human liver remains unclear. In the present study, messenger RNA sequencing (mRNA-seq), microRNA sequencing (miRNA-seq), and real-time qPCR were used to distinguish the expression differences between control and naringenin-treated HepaRG cells. We obtained 1037 differentially expressed mRNAs and 234 miRNAs. According to the target prediction and integration analysis in silico, we found 20 potential miRNA-mRNA pairs involved in liver metabolism. This study is the first to provide a perspective of miRNA–mRNA interactions in the regulation of naringenin via an integrated analysis of mRNA-seq and miRNA-seq in HepaRG cells, which further characterizes the nutraceutical value of naringenin as a food additive. MDPI 2021-02-25 /pmc/articles/PMC7956767/ /pubmed/33669020 http://dx.doi.org/10.3390/ijms22052292 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fan, Weiyang
Shi, Rui
Guan, Minyi
Chen, Pan
Wu, Hao
Su, Weiwei
Wang, Yonggang
Li, Peibo
The Effects of Naringenin on miRNA-mRNA Profiles in HepaRG Cells
title The Effects of Naringenin on miRNA-mRNA Profiles in HepaRG Cells
title_full The Effects of Naringenin on miRNA-mRNA Profiles in HepaRG Cells
title_fullStr The Effects of Naringenin on miRNA-mRNA Profiles in HepaRG Cells
title_full_unstemmed The Effects of Naringenin on miRNA-mRNA Profiles in HepaRG Cells
title_short The Effects of Naringenin on miRNA-mRNA Profiles in HepaRG Cells
title_sort effects of naringenin on mirna-mrna profiles in heparg cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956767/
https://www.ncbi.nlm.nih.gov/pubmed/33669020
http://dx.doi.org/10.3390/ijms22052292
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