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Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions

Glucose is a major energy source consumed by proliferating mammalian cells. Therefore, in general, proliferating cells have the preference of high glucose contents in extracellular environment. Here, we showed that high glucose concentrations impede the proliferation of satellite cells, which are mu...

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Autores principales: Furuichi, Yasuro, Kawabata, Yuki, Aoki, Miho, Mita, Yoshitaka, Fujii, Nobuharu L., Manabe, Yasuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957019/
https://www.ncbi.nlm.nih.gov/pubmed/33732705
http://dx.doi.org/10.3389/fcell.2021.640399
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author Furuichi, Yasuro
Kawabata, Yuki
Aoki, Miho
Mita, Yoshitaka
Fujii, Nobuharu L.
Manabe, Yasuko
author_facet Furuichi, Yasuro
Kawabata, Yuki
Aoki, Miho
Mita, Yoshitaka
Fujii, Nobuharu L.
Manabe, Yasuko
author_sort Furuichi, Yasuro
collection PubMed
description Glucose is a major energy source consumed by proliferating mammalian cells. Therefore, in general, proliferating cells have the preference of high glucose contents in extracellular environment. Here, we showed that high glucose concentrations impede the proliferation of satellite cells, which are muscle-specific stem cells, under adherent culture conditions. We found that the proliferation activity of satellite cells was higher in glucose-free DMEM growth medium (low-glucose medium with a glucose concentration of 2 mM) than in standard glucose DMEM (high-glucose medium with a glucose concentration of 19 mM). Satellite cells cultured in the high-glucose medium showed a decreased population of reserve cells, identified by staining for Pax7 expression, suggesting that glucose concentration affects cell fate determination. In conclusion, glucose is a factor that decides the cell fate of skeletal muscle-specific stem cells. Due to this unique feature of satellite cells, hyperglycemia may negatively affect the regenerative capability of skeletal muscle myofibers and thus facilitate sarcopenia.
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spelling pubmed-79570192021-03-16 Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions Furuichi, Yasuro Kawabata, Yuki Aoki, Miho Mita, Yoshitaka Fujii, Nobuharu L. Manabe, Yasuko Front Cell Dev Biol Cell and Developmental Biology Glucose is a major energy source consumed by proliferating mammalian cells. Therefore, in general, proliferating cells have the preference of high glucose contents in extracellular environment. Here, we showed that high glucose concentrations impede the proliferation of satellite cells, which are muscle-specific stem cells, under adherent culture conditions. We found that the proliferation activity of satellite cells was higher in glucose-free DMEM growth medium (low-glucose medium with a glucose concentration of 2 mM) than in standard glucose DMEM (high-glucose medium with a glucose concentration of 19 mM). Satellite cells cultured in the high-glucose medium showed a decreased population of reserve cells, identified by staining for Pax7 expression, suggesting that glucose concentration affects cell fate determination. In conclusion, glucose is a factor that decides the cell fate of skeletal muscle-specific stem cells. Due to this unique feature of satellite cells, hyperglycemia may negatively affect the regenerative capability of skeletal muscle myofibers and thus facilitate sarcopenia. Frontiers Media S.A. 2021-03-01 /pmc/articles/PMC7957019/ /pubmed/33732705 http://dx.doi.org/10.3389/fcell.2021.640399 Text en Copyright © 2021 Furuichi, Kawabata, Aoki, Mita, Fujii and Manabe. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Furuichi, Yasuro
Kawabata, Yuki
Aoki, Miho
Mita, Yoshitaka
Fujii, Nobuharu L.
Manabe, Yasuko
Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions
title Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions
title_full Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions
title_fullStr Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions
title_full_unstemmed Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions
title_short Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions
title_sort excess glucose impedes the proliferation of skeletal muscle satellite cells under adherent culture conditions
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957019/
https://www.ncbi.nlm.nih.gov/pubmed/33732705
http://dx.doi.org/10.3389/fcell.2021.640399
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