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Molecular Subtypes Based on the Stemness Index Predict Prognosis in Glioma Patients
Glioma is the common histological subtype of malignancy in the central nervous system, with high morbidity and mortality. Glioma cancer stem cells (CSCs) play essential roles in tumor recurrence and treatment resistance. Thus, exploring the stem cell-related genes and subtypes in glioma is important...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957071/ https://www.ncbi.nlm.nih.gov/pubmed/33732284 http://dx.doi.org/10.3389/fgene.2021.616507 |
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author | Tan, Jun Zhu, Hecheng Tang, Guihua Liu, Hongwei Wanggou, Siyi Cao, Yudong Xin, Zhaoqi Zhou, Quanwei Zhan, Chaohong Wu, Zhaoping Guo, Youwei Jiang, Zhipeng Zhao, Ming Ren, Caiping Jiang, Xingjun Yin, Wen |
author_facet | Tan, Jun Zhu, Hecheng Tang, Guihua Liu, Hongwei Wanggou, Siyi Cao, Yudong Xin, Zhaoqi Zhou, Quanwei Zhan, Chaohong Wu, Zhaoping Guo, Youwei Jiang, Zhipeng Zhao, Ming Ren, Caiping Jiang, Xingjun Yin, Wen |
author_sort | Tan, Jun |
collection | PubMed |
description | Glioma is the common histological subtype of malignancy in the central nervous system, with high morbidity and mortality. Glioma cancer stem cells (CSCs) play essential roles in tumor recurrence and treatment resistance. Thus, exploring the stem cell-related genes and subtypes in glioma is important. In this study, we collected the RNA-sequencing (RNA-seq) data and clinical information of glioma patients from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) databases. With the differentially expressed genes (DEGs) and weighted gene correlation network analysis (WGCNA), we identified 86 mRNA expression-based stemness index (mRNAsi)-related genes in 583 samples from TCGA RNA-seq dataset. Furthermore, these samples from TCGA database could be divided into two significantly different subtypes with different prognoses based on the mRNAsi corresponding gene, which could also be validated in the CGGA database. The clinical characteristics and immune cell infiltrate distribution of the two stemness subtypes are different. Then, functional enrichment analyses were performed to identify the different gene ontology (GO) terms and pathways in the two different subtypes. Moreover, we constructed a stemness subtype-related risk score model and nomogram to predict the prognosis of glioma patients. Finally, we selected one gene (ETV2) from the risk score model for experimental validation. The results showed that ETV2 can contribute to the invasion, migration, and epithelial-mesenchymal transition (EMT) process of glioma. In conclusion, we identified two distinct molecular subtypes and potential therapeutic targets of glioma, which could provide new insights for the development of precision diagnosis and prognostic prediction for glioma patients. |
format | Online Article Text |
id | pubmed-7957071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79570712021-03-16 Molecular Subtypes Based on the Stemness Index Predict Prognosis in Glioma Patients Tan, Jun Zhu, Hecheng Tang, Guihua Liu, Hongwei Wanggou, Siyi Cao, Yudong Xin, Zhaoqi Zhou, Quanwei Zhan, Chaohong Wu, Zhaoping Guo, Youwei Jiang, Zhipeng Zhao, Ming Ren, Caiping Jiang, Xingjun Yin, Wen Front Genet Genetics Glioma is the common histological subtype of malignancy in the central nervous system, with high morbidity and mortality. Glioma cancer stem cells (CSCs) play essential roles in tumor recurrence and treatment resistance. Thus, exploring the stem cell-related genes and subtypes in glioma is important. In this study, we collected the RNA-sequencing (RNA-seq) data and clinical information of glioma patients from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) databases. With the differentially expressed genes (DEGs) and weighted gene correlation network analysis (WGCNA), we identified 86 mRNA expression-based stemness index (mRNAsi)-related genes in 583 samples from TCGA RNA-seq dataset. Furthermore, these samples from TCGA database could be divided into two significantly different subtypes with different prognoses based on the mRNAsi corresponding gene, which could also be validated in the CGGA database. The clinical characteristics and immune cell infiltrate distribution of the two stemness subtypes are different. Then, functional enrichment analyses were performed to identify the different gene ontology (GO) terms and pathways in the two different subtypes. Moreover, we constructed a stemness subtype-related risk score model and nomogram to predict the prognosis of glioma patients. Finally, we selected one gene (ETV2) from the risk score model for experimental validation. The results showed that ETV2 can contribute to the invasion, migration, and epithelial-mesenchymal transition (EMT) process of glioma. In conclusion, we identified two distinct molecular subtypes and potential therapeutic targets of glioma, which could provide new insights for the development of precision diagnosis and prognostic prediction for glioma patients. Frontiers Media S.A. 2021-03-01 /pmc/articles/PMC7957071/ /pubmed/33732284 http://dx.doi.org/10.3389/fgene.2021.616507 Text en Copyright © 2021 Tan, Zhu, Tang, Liu, Wanggou, Cao, Xin, Zhou, Zhan, Wu, Guo, Jiang, Zhao, Ren, Jiang and Yin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Tan, Jun Zhu, Hecheng Tang, Guihua Liu, Hongwei Wanggou, Siyi Cao, Yudong Xin, Zhaoqi Zhou, Quanwei Zhan, Chaohong Wu, Zhaoping Guo, Youwei Jiang, Zhipeng Zhao, Ming Ren, Caiping Jiang, Xingjun Yin, Wen Molecular Subtypes Based on the Stemness Index Predict Prognosis in Glioma Patients |
title | Molecular Subtypes Based on the Stemness Index Predict Prognosis in Glioma Patients |
title_full | Molecular Subtypes Based on the Stemness Index Predict Prognosis in Glioma Patients |
title_fullStr | Molecular Subtypes Based on the Stemness Index Predict Prognosis in Glioma Patients |
title_full_unstemmed | Molecular Subtypes Based on the Stemness Index Predict Prognosis in Glioma Patients |
title_short | Molecular Subtypes Based on the Stemness Index Predict Prognosis in Glioma Patients |
title_sort | molecular subtypes based on the stemness index predict prognosis in glioma patients |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957071/ https://www.ncbi.nlm.nih.gov/pubmed/33732284 http://dx.doi.org/10.3389/fgene.2021.616507 |
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