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Resveratrol Affects Insulin Signaling in Type 2 Diabetic Goto-Kakizaki Rats

Resveratrol is a biologically active diphenolic compound exerting multiple beneficial effects in the organism, including anti-diabetic properties. This action is, however, not fully elucidated. In the present study, we examined effects of resveratrol on some parameters related to insulin signaling,...

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Autores principales: Szkudelska, Katarzyna, Deniziak, Marzanna, Sassek, Maciej, Szkudelski, Ignacy, Noskowiak, Wojciech, Szkudelski, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957525/
https://www.ncbi.nlm.nih.gov/pubmed/33671110
http://dx.doi.org/10.3390/ijms22052469
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author Szkudelska, Katarzyna
Deniziak, Marzanna
Sassek, Maciej
Szkudelski, Ignacy
Noskowiak, Wojciech
Szkudelski, Tomasz
author_facet Szkudelska, Katarzyna
Deniziak, Marzanna
Sassek, Maciej
Szkudelski, Ignacy
Noskowiak, Wojciech
Szkudelski, Tomasz
author_sort Szkudelska, Katarzyna
collection PubMed
description Resveratrol is a biologically active diphenolic compound exerting multiple beneficial effects in the organism, including anti-diabetic properties. This action is, however, not fully elucidated. In the present study, we examined effects of resveratrol on some parameters related to insulin signaling, and also on diabetes-associated dysregulation in Goto-Kakizaki (GK) rats with congenital type 2 diabetes. Resveratrol was given at the dose of 20 mg/kg b.w. for 10 weeks. It was shown that the expression and phosphorylation levels of insulin receptor in the skeletal muscle of GK rats were significantly decreased, compared with control animals. However, these changes were totally prevented by resveratrol. Liver expression of the insulin receptor was also reduced, but in this case, resveratrol was ineffective. Resveratrol was also demonstrated to significantly influence parameters of insulin binding (dissociation constant and binding capacity) in the skeletal muscle and liver. Moreover, it was shown that the expression levels of proteins related to intracellular glucose transport (GLUT4 and TUG) in adipose tissue of GK rats were significantly decreased. However, treatment with resveratrol completely abolished these changes. Resveratrol was found to induce normalization of TUG expression in the skeletal muscle. Blood levels of insulin and GIP were elevated, whereas proinsulin and GLP-1 diminished in GK rats. However, concentrations of these hormones were not affected by resveratrol. These results indicate that resveratrol partially ameliorates diabetes-associated dysregulation in GK rats. The most relevant finding covers the normalization of the insulin receptor expression in the skeletal muscle and also GLUT4 and TUG in adipose tissue.
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spelling pubmed-79575252021-03-16 Resveratrol Affects Insulin Signaling in Type 2 Diabetic Goto-Kakizaki Rats Szkudelska, Katarzyna Deniziak, Marzanna Sassek, Maciej Szkudelski, Ignacy Noskowiak, Wojciech Szkudelski, Tomasz Int J Mol Sci Article Resveratrol is a biologically active diphenolic compound exerting multiple beneficial effects in the organism, including anti-diabetic properties. This action is, however, not fully elucidated. In the present study, we examined effects of resveratrol on some parameters related to insulin signaling, and also on diabetes-associated dysregulation in Goto-Kakizaki (GK) rats with congenital type 2 diabetes. Resveratrol was given at the dose of 20 mg/kg b.w. for 10 weeks. It was shown that the expression and phosphorylation levels of insulin receptor in the skeletal muscle of GK rats were significantly decreased, compared with control animals. However, these changes were totally prevented by resveratrol. Liver expression of the insulin receptor was also reduced, but in this case, resveratrol was ineffective. Resveratrol was also demonstrated to significantly influence parameters of insulin binding (dissociation constant and binding capacity) in the skeletal muscle and liver. Moreover, it was shown that the expression levels of proteins related to intracellular glucose transport (GLUT4 and TUG) in adipose tissue of GK rats were significantly decreased. However, treatment with resveratrol completely abolished these changes. Resveratrol was found to induce normalization of TUG expression in the skeletal muscle. Blood levels of insulin and GIP were elevated, whereas proinsulin and GLP-1 diminished in GK rats. However, concentrations of these hormones were not affected by resveratrol. These results indicate that resveratrol partially ameliorates diabetes-associated dysregulation in GK rats. The most relevant finding covers the normalization of the insulin receptor expression in the skeletal muscle and also GLUT4 and TUG in adipose tissue. MDPI 2021-02-28 /pmc/articles/PMC7957525/ /pubmed/33671110 http://dx.doi.org/10.3390/ijms22052469 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szkudelska, Katarzyna
Deniziak, Marzanna
Sassek, Maciej
Szkudelski, Ignacy
Noskowiak, Wojciech
Szkudelski, Tomasz
Resveratrol Affects Insulin Signaling in Type 2 Diabetic Goto-Kakizaki Rats
title Resveratrol Affects Insulin Signaling in Type 2 Diabetic Goto-Kakizaki Rats
title_full Resveratrol Affects Insulin Signaling in Type 2 Diabetic Goto-Kakizaki Rats
title_fullStr Resveratrol Affects Insulin Signaling in Type 2 Diabetic Goto-Kakizaki Rats
title_full_unstemmed Resveratrol Affects Insulin Signaling in Type 2 Diabetic Goto-Kakizaki Rats
title_short Resveratrol Affects Insulin Signaling in Type 2 Diabetic Goto-Kakizaki Rats
title_sort resveratrol affects insulin signaling in type 2 diabetic goto-kakizaki rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957525/
https://www.ncbi.nlm.nih.gov/pubmed/33671110
http://dx.doi.org/10.3390/ijms22052469
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