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An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies
This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose,...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957696/ https://www.ncbi.nlm.nih.gov/pubmed/33671059 http://dx.doi.org/10.3390/polym13050762 |
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author | Omar, Nur Alia Sheh Fen, Yap Wing Ramli, Irmawati Sadrolhosseini, Amir Reza Abdullah, Jaafar Yusof, Nor Azah Kamil, Yasmin Mustapha Mahdi, Mohd Adzir |
author_facet | Omar, Nur Alia Sheh Fen, Yap Wing Ramli, Irmawati Sadrolhosseini, Amir Reza Abdullah, Jaafar Yusof, Nor Azah Kamil, Yasmin Mustapha Mahdi, Mohd Adzir |
author_sort | Omar, Nur Alia Sheh |
collection | PubMed |
description | This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose, two ranges of DENV-2 E-protein concentrations, i.e., 0.000008–0.0001 nM and 0.00008–0.005 nM were evaluated, and the lowest detectable concentration was achieved at 0.00008 nM. The incorporation of PAMAM dendrimer-based nanocomposite thin film with an SPR sensor exhibited a significant increase in sensitivity and binding affinity to a lower range DENV-2 E-protein concentrations. Moreover, the proposed sensor displayed good selectivity towards DENV-2 E-proteins and have an average recovery of 80–120%. The findings of this study demonstrated that PAMAM dendrimer-based nanocomposite thin film combined with SPR sensor is a promising diagnostic tool for sensitive and selective detection of DENV-2 E-proteins. |
format | Online Article Text |
id | pubmed-7957696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79576962021-03-16 An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies Omar, Nur Alia Sheh Fen, Yap Wing Ramli, Irmawati Sadrolhosseini, Amir Reza Abdullah, Jaafar Yusof, Nor Azah Kamil, Yasmin Mustapha Mahdi, Mohd Adzir Polymers (Basel) Article This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose, two ranges of DENV-2 E-protein concentrations, i.e., 0.000008–0.0001 nM and 0.00008–0.005 nM were evaluated, and the lowest detectable concentration was achieved at 0.00008 nM. The incorporation of PAMAM dendrimer-based nanocomposite thin film with an SPR sensor exhibited a significant increase in sensitivity and binding affinity to a lower range DENV-2 E-protein concentrations. Moreover, the proposed sensor displayed good selectivity towards DENV-2 E-proteins and have an average recovery of 80–120%. The findings of this study demonstrated that PAMAM dendrimer-based nanocomposite thin film combined with SPR sensor is a promising diagnostic tool for sensitive and selective detection of DENV-2 E-proteins. MDPI 2021-02-28 /pmc/articles/PMC7957696/ /pubmed/33671059 http://dx.doi.org/10.3390/polym13050762 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Omar, Nur Alia Sheh Fen, Yap Wing Ramli, Irmawati Sadrolhosseini, Amir Reza Abdullah, Jaafar Yusof, Nor Azah Kamil, Yasmin Mustapha Mahdi, Mohd Adzir An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies |
title | An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies |
title_full | An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies |
title_fullStr | An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies |
title_full_unstemmed | An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies |
title_short | An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies |
title_sort | optical sensor for dengue envelope proteins using polyamidoamine dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957696/ https://www.ncbi.nlm.nih.gov/pubmed/33671059 http://dx.doi.org/10.3390/polym13050762 |
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