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An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose,...

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Autores principales: Omar, Nur Alia Sheh, Fen, Yap Wing, Ramli, Irmawati, Sadrolhosseini, Amir Reza, Abdullah, Jaafar, Yusof, Nor Azah, Kamil, Yasmin Mustapha, Mahdi, Mohd Adzir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957696/
https://www.ncbi.nlm.nih.gov/pubmed/33671059
http://dx.doi.org/10.3390/polym13050762
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author Omar, Nur Alia Sheh
Fen, Yap Wing
Ramli, Irmawati
Sadrolhosseini, Amir Reza
Abdullah, Jaafar
Yusof, Nor Azah
Kamil, Yasmin Mustapha
Mahdi, Mohd Adzir
author_facet Omar, Nur Alia Sheh
Fen, Yap Wing
Ramli, Irmawati
Sadrolhosseini, Amir Reza
Abdullah, Jaafar
Yusof, Nor Azah
Kamil, Yasmin Mustapha
Mahdi, Mohd Adzir
author_sort Omar, Nur Alia Sheh
collection PubMed
description This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose, two ranges of DENV-2 E-protein concentrations, i.e., 0.000008–0.0001 nM and 0.00008–0.005 nM were evaluated, and the lowest detectable concentration was achieved at 0.00008 nM. The incorporation of PAMAM dendrimer-based nanocomposite thin film with an SPR sensor exhibited a significant increase in sensitivity and binding affinity to a lower range DENV-2 E-protein concentrations. Moreover, the proposed sensor displayed good selectivity towards DENV-2 E-proteins and have an average recovery of 80–120%. The findings of this study demonstrated that PAMAM dendrimer-based nanocomposite thin film combined with SPR sensor is a promising diagnostic tool for sensitive and selective detection of DENV-2 E-proteins.
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spelling pubmed-79576962021-03-16 An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies Omar, Nur Alia Sheh Fen, Yap Wing Ramli, Irmawati Sadrolhosseini, Amir Reza Abdullah, Jaafar Yusof, Nor Azah Kamil, Yasmin Mustapha Mahdi, Mohd Adzir Polymers (Basel) Article This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose, two ranges of DENV-2 E-protein concentrations, i.e., 0.000008–0.0001 nM and 0.00008–0.005 nM were evaluated, and the lowest detectable concentration was achieved at 0.00008 nM. The incorporation of PAMAM dendrimer-based nanocomposite thin film with an SPR sensor exhibited a significant increase in sensitivity and binding affinity to a lower range DENV-2 E-protein concentrations. Moreover, the proposed sensor displayed good selectivity towards DENV-2 E-proteins and have an average recovery of 80–120%. The findings of this study demonstrated that PAMAM dendrimer-based nanocomposite thin film combined with SPR sensor is a promising diagnostic tool for sensitive and selective detection of DENV-2 E-proteins. MDPI 2021-02-28 /pmc/articles/PMC7957696/ /pubmed/33671059 http://dx.doi.org/10.3390/polym13050762 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Omar, Nur Alia Sheh
Fen, Yap Wing
Ramli, Irmawati
Sadrolhosseini, Amir Reza
Abdullah, Jaafar
Yusof, Nor Azah
Kamil, Yasmin Mustapha
Mahdi, Mohd Adzir
An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies
title An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies
title_full An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies
title_fullStr An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies
title_full_unstemmed An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies
title_short An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies
title_sort optical sensor for dengue envelope proteins using polyamidoamine dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957696/
https://www.ncbi.nlm.nih.gov/pubmed/33671059
http://dx.doi.org/10.3390/polym13050762
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