Cargando…

Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers

The rapid spread of the Coronavirus Disease 2019 (COVID-19) pandemic, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pathogen has generated a huge international public health emergency. Currently the reference diagnostic technique for virus determination is Reverse Transc...

Descripción completa

Detalles Bibliográficos
Autores principales: Cennamo, Nunzio, D’Agostino, Girolamo, Perri, Chiara, Arcadio, Francesco, Chiaretti, Guido, Parisio, Eva Maria, Camarlinghi, Giulio, Vettori, Chiara, Di Marzo, Francesco, Cennamo, Rosario, Porto, Giovanni, Zeni, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957720/
https://www.ncbi.nlm.nih.gov/pubmed/33804378
http://dx.doi.org/10.3390/s21051681
_version_ 1783664714210869248
author Cennamo, Nunzio
D’Agostino, Girolamo
Perri, Chiara
Arcadio, Francesco
Chiaretti, Guido
Parisio, Eva Maria
Camarlinghi, Giulio
Vettori, Chiara
Di Marzo, Francesco
Cennamo, Rosario
Porto, Giovanni
Zeni, Luigi
author_facet Cennamo, Nunzio
D’Agostino, Girolamo
Perri, Chiara
Arcadio, Francesco
Chiaretti, Guido
Parisio, Eva Maria
Camarlinghi, Giulio
Vettori, Chiara
Di Marzo, Francesco
Cennamo, Rosario
Porto, Giovanni
Zeni, Luigi
author_sort Cennamo, Nunzio
collection PubMed
description The rapid spread of the Coronavirus Disease 2019 (COVID-19) pandemic, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pathogen has generated a huge international public health emergency. Currently the reference diagnostic technique for virus determination is Reverse Transcription Polymerase Chain Reaction (RT-PCR) real time analysis that requires specialized equipment, reagents and facilities and typically 3–4 h to perform. Thus, the realization of simple, low-cost, small-size, rapid and point-of-care diagnostics tests has become a global priority. In response to the current need for quick, highly sensitive and on-site detection of the SARS-CoV-2 virus in several aqueous solutions, a specific molecularly imprinted polymer (MIP) receptor has been designed, realized, and combined with an optical sensor. More specifically, the proof of concept of a SARS-CoV-2 sensor has been demonstrated by exploiting a plasmonic plastic optical fiber sensor coupled with a novel kind of synthetic MIP nano-layer, especially designed for the specific recognition of Subunit 1 of the SARS-CoV-2 Spike protein. First, we have tested the effectiveness of the developed MIP receptor to bind the Subunit 1 of the SARS-CoV-2 spike protein, then the results of preliminary tests on SARS-CoV-2 virions, performed on samples of nasopharyngeal (NP) swabs in universal transport medium (UTM) and physiological solution (0.9% NaCl), were compared with those obtained with RT-PCR. According to these preliminary results, the sensitivity of the proposed optical-chemical sensor proved to be higher than the RT-PCR one. Furthermore, a relatively fast response time (about 10 min) to the virus was obtained without the use of additional reagents.
format Online
Article
Text
id pubmed-7957720
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79577202021-03-16 Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers Cennamo, Nunzio D’Agostino, Girolamo Perri, Chiara Arcadio, Francesco Chiaretti, Guido Parisio, Eva Maria Camarlinghi, Giulio Vettori, Chiara Di Marzo, Francesco Cennamo, Rosario Porto, Giovanni Zeni, Luigi Sensors (Basel) Article The rapid spread of the Coronavirus Disease 2019 (COVID-19) pandemic, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pathogen has generated a huge international public health emergency. Currently the reference diagnostic technique for virus determination is Reverse Transcription Polymerase Chain Reaction (RT-PCR) real time analysis that requires specialized equipment, reagents and facilities and typically 3–4 h to perform. Thus, the realization of simple, low-cost, small-size, rapid and point-of-care diagnostics tests has become a global priority. In response to the current need for quick, highly sensitive and on-site detection of the SARS-CoV-2 virus in several aqueous solutions, a specific molecularly imprinted polymer (MIP) receptor has been designed, realized, and combined with an optical sensor. More specifically, the proof of concept of a SARS-CoV-2 sensor has been demonstrated by exploiting a plasmonic plastic optical fiber sensor coupled with a novel kind of synthetic MIP nano-layer, especially designed for the specific recognition of Subunit 1 of the SARS-CoV-2 Spike protein. First, we have tested the effectiveness of the developed MIP receptor to bind the Subunit 1 of the SARS-CoV-2 spike protein, then the results of preliminary tests on SARS-CoV-2 virions, performed on samples of nasopharyngeal (NP) swabs in universal transport medium (UTM) and physiological solution (0.9% NaCl), were compared with those obtained with RT-PCR. According to these preliminary results, the sensitivity of the proposed optical-chemical sensor proved to be higher than the RT-PCR one. Furthermore, a relatively fast response time (about 10 min) to the virus was obtained without the use of additional reagents. MDPI 2021-03-01 /pmc/articles/PMC7957720/ /pubmed/33804378 http://dx.doi.org/10.3390/s21051681 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cennamo, Nunzio
D’Agostino, Girolamo
Perri, Chiara
Arcadio, Francesco
Chiaretti, Guido
Parisio, Eva Maria
Camarlinghi, Giulio
Vettori, Chiara
Di Marzo, Francesco
Cennamo, Rosario
Porto, Giovanni
Zeni, Luigi
Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers
title Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers
title_full Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers
title_fullStr Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers
title_full_unstemmed Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers
title_short Proof of Concept for a Quick and Highly Sensitive On-Site Detection of SARS-CoV-2 by Plasmonic Optical Fibers and Molecularly Imprinted Polymers
title_sort proof of concept for a quick and highly sensitive on-site detection of sars-cov-2 by plasmonic optical fibers and molecularly imprinted polymers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957720/
https://www.ncbi.nlm.nih.gov/pubmed/33804378
http://dx.doi.org/10.3390/s21051681
work_keys_str_mv AT cennamonunzio proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT dagostinogirolamo proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT perrichiara proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT arcadiofrancesco proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT chiarettiguido proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT parisioevamaria proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT camarlinghigiulio proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT vettorichiara proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT dimarzofrancesco proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT cennamorosario proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT portogiovanni proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers
AT zeniluigi proofofconceptforaquickandhighlysensitiveonsitedetectionofsarscov2byplasmonicopticalfibersandmolecularlyimprintedpolymers