Cargando…

Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111

Probiotics make up a large and growing segment of the commercial market of dietary supplements and are touted as offering a variety of human health benefits. Some of the purported positive impacts of probiotics include, but are not limited to, stabilization of the gut microbiota, prevention of gastr...

Descripción completa

Detalles Bibliográficos
Autores principales: Freedman, Kimberley E., Hill, Jessica L., Wei, Yuren, Vazquez, Allegra R., Grubb, Diana S., Trotter, Roxanne E., Wrigley, Scott D., Johnson, Sarah A., Foster, Michelle T., Weir, Tiffany L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957723/
https://www.ncbi.nlm.nih.gov/pubmed/33671071
http://dx.doi.org/10.3390/ijms22052453
_version_ 1783664714952212480
author Freedman, Kimberley E.
Hill, Jessica L.
Wei, Yuren
Vazquez, Allegra R.
Grubb, Diana S.
Trotter, Roxanne E.
Wrigley, Scott D.
Johnson, Sarah A.
Foster, Michelle T.
Weir, Tiffany L.
author_facet Freedman, Kimberley E.
Hill, Jessica L.
Wei, Yuren
Vazquez, Allegra R.
Grubb, Diana S.
Trotter, Roxanne E.
Wrigley, Scott D.
Johnson, Sarah A.
Foster, Michelle T.
Weir, Tiffany L.
author_sort Freedman, Kimberley E.
collection PubMed
description Probiotics make up a large and growing segment of the commercial market of dietary supplements and are touted as offering a variety of human health benefits. Some of the purported positive impacts of probiotics include, but are not limited to, stabilization of the gut microbiota, prevention of gastrointestinal disorders and modulation of the host immune system. Current research suggests that the immunomodulatory effects of probiotics are strain-specific and vary in mode of action. Here, we examined the immunomodulatory properties of Bacillus subtilis strain DE111 in a healthy human population. In a pilot randomized, double blind, placebo-controlled four-week intervention, we examined peripheral blood mononuclear cells (PBMCs) at basal levels pre- and post-intervention, as well as in response to stimulation with bacterial lipopolysaccharide (LPS). We observed an increase in anti-inflammatory immune cell populations in response to ex vivo LPS stimulation of PBMCs in the DE111 intervention group. Overall perceived gastrointestinal health, microbiota, and circulating and fecal markers of inflammation (Il-6, sIgA) and gut barrier function (plasma zonulin) were largely unaffected by DE111 intervention, although the study may have been underpowered to detect these differences. These pilot data provide information and justification to conduct an appropriately powered clinical study to further examine the immunomodulatory potential of B. subtilis DE111 in human populations.
format Online
Article
Text
id pubmed-7957723
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-79577232021-03-16 Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111 Freedman, Kimberley E. Hill, Jessica L. Wei, Yuren Vazquez, Allegra R. Grubb, Diana S. Trotter, Roxanne E. Wrigley, Scott D. Johnson, Sarah A. Foster, Michelle T. Weir, Tiffany L. Int J Mol Sci Article Probiotics make up a large and growing segment of the commercial market of dietary supplements and are touted as offering a variety of human health benefits. Some of the purported positive impacts of probiotics include, but are not limited to, stabilization of the gut microbiota, prevention of gastrointestinal disorders and modulation of the host immune system. Current research suggests that the immunomodulatory effects of probiotics are strain-specific and vary in mode of action. Here, we examined the immunomodulatory properties of Bacillus subtilis strain DE111 in a healthy human population. In a pilot randomized, double blind, placebo-controlled four-week intervention, we examined peripheral blood mononuclear cells (PBMCs) at basal levels pre- and post-intervention, as well as in response to stimulation with bacterial lipopolysaccharide (LPS). We observed an increase in anti-inflammatory immune cell populations in response to ex vivo LPS stimulation of PBMCs in the DE111 intervention group. Overall perceived gastrointestinal health, microbiota, and circulating and fecal markers of inflammation (Il-6, sIgA) and gut barrier function (plasma zonulin) were largely unaffected by DE111 intervention, although the study may have been underpowered to detect these differences. These pilot data provide information and justification to conduct an appropriately powered clinical study to further examine the immunomodulatory potential of B. subtilis DE111 in human populations. MDPI 2021-02-28 /pmc/articles/PMC7957723/ /pubmed/33671071 http://dx.doi.org/10.3390/ijms22052453 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Freedman, Kimberley E.
Hill, Jessica L.
Wei, Yuren
Vazquez, Allegra R.
Grubb, Diana S.
Trotter, Roxanne E.
Wrigley, Scott D.
Johnson, Sarah A.
Foster, Michelle T.
Weir, Tiffany L.
Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111
title Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111
title_full Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111
title_fullStr Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111
title_full_unstemmed Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111
title_short Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111
title_sort examining the gastrointestinal and immunomodulatory effects of the novel probiotic bacillus subtilis de111
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957723/
https://www.ncbi.nlm.nih.gov/pubmed/33671071
http://dx.doi.org/10.3390/ijms22052453
work_keys_str_mv AT freedmankimberleye examiningthegastrointestinalandimmunomodulatoryeffectsofthenovelprobioticbacillussubtilisde111
AT hilljessical examiningthegastrointestinalandimmunomodulatoryeffectsofthenovelprobioticbacillussubtilisde111
AT weiyuren examiningthegastrointestinalandimmunomodulatoryeffectsofthenovelprobioticbacillussubtilisde111
AT vazquezallegrar examiningthegastrointestinalandimmunomodulatoryeffectsofthenovelprobioticbacillussubtilisde111
AT grubbdianas examiningthegastrointestinalandimmunomodulatoryeffectsofthenovelprobioticbacillussubtilisde111
AT trotterroxannee examiningthegastrointestinalandimmunomodulatoryeffectsofthenovelprobioticbacillussubtilisde111
AT wrigleyscottd examiningthegastrointestinalandimmunomodulatoryeffectsofthenovelprobioticbacillussubtilisde111
AT johnsonsaraha examiningthegastrointestinalandimmunomodulatoryeffectsofthenovelprobioticbacillussubtilisde111
AT fostermichellet examiningthegastrointestinalandimmunomodulatoryeffectsofthenovelprobioticbacillussubtilisde111
AT weirtiffanyl examiningthegastrointestinalandimmunomodulatoryeffectsofthenovelprobioticbacillussubtilisde111