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Controlling Ratios of Plasmid-Based Double Cut Donor and CRISPR/Cas9 Components to Enhance Targeted Integration of Transgenes in Chinese Hamster Ovary Cells

Chinese hamster ovary (CHO) cells are the most valuable expression host for the commercial production of biotherapeutics. Recent trends in recombinant CHO cell-line development have focused on the site-specific integration of transgenes encoding recombinant proteins over random integration. However,...

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Detalles Bibliográficos
Autores principales:	Shin, Sung Wook, Kim, Dongwoo, Lee, Jae Seong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957797/ https://www.ncbi.nlm.nih.gov/pubmed/33673701 http://dx.doi.org/10.3390/ijms22052407

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