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Zika Virus Growth in Human Kidney Cells Is Restricted by an Elevated Glucose Level

Mosquito-borne Zika virus (ZIKV) became a real threat to human health due to the lack of vaccine and effective antiviral treatment. The virus has recently been responsible for a global outbreak leading to millions of infected cases. ZIKV complications were highlighted in adults with Guillain–Barré s...

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Autores principales: Reslan, Alawiya, Haddad, Juliano G., Moukendza Koundi, Liadrine, Desprès, Philippe, Bascands, Jean-Loup, Gadea, Gilles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958337/
https://www.ncbi.nlm.nih.gov/pubmed/33801335
http://dx.doi.org/10.3390/ijms22052495
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author Reslan, Alawiya
Haddad, Juliano G.
Moukendza Koundi, Liadrine
Desprès, Philippe
Bascands, Jean-Loup
Gadea, Gilles
author_facet Reslan, Alawiya
Haddad, Juliano G.
Moukendza Koundi, Liadrine
Desprès, Philippe
Bascands, Jean-Loup
Gadea, Gilles
author_sort Reslan, Alawiya
collection PubMed
description Mosquito-borne Zika virus (ZIKV) became a real threat to human health due to the lack of vaccine and effective antiviral treatment. The virus has recently been responsible for a global outbreak leading to millions of infected cases. ZIKV complications were highlighted in adults with Guillain–Barré syndrome and in newborns with increasing numbers of congenital disorders ranging from mild developmental delays to fatal conditions. The ability of ZIKV to establish a long-term infection in diverse organs including the kidneys has been recently documented but the consequences of such a viral infection are still debated. Our study aimed to determine whether the efficiency of ZIKV growth in kidney cells relates to glucose concentration. Human kidney HK-2 cells were infected with different ZIKV strains in presence of normal and high glucose concentrations. Virological assays showed a decrease in viral replication without modifying entry steps (viral binding, internalization, fusion) under high glucose conditions. This decrease replication was associated with a lower virus progeny and increased cell viability when compared to ZIKV-infected HK-2 cells in normal glucose concentration. In conclusion, we showed for the first time that an elevated glucose level influences ZIKV replication level with an effect on kidney cell survival.
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spelling pubmed-79583372021-03-16 Zika Virus Growth in Human Kidney Cells Is Restricted by an Elevated Glucose Level Reslan, Alawiya Haddad, Juliano G. Moukendza Koundi, Liadrine Desprès, Philippe Bascands, Jean-Loup Gadea, Gilles Int J Mol Sci Article Mosquito-borne Zika virus (ZIKV) became a real threat to human health due to the lack of vaccine and effective antiviral treatment. The virus has recently been responsible for a global outbreak leading to millions of infected cases. ZIKV complications were highlighted in adults with Guillain–Barré syndrome and in newborns with increasing numbers of congenital disorders ranging from mild developmental delays to fatal conditions. The ability of ZIKV to establish a long-term infection in diverse organs including the kidneys has been recently documented but the consequences of such a viral infection are still debated. Our study aimed to determine whether the efficiency of ZIKV growth in kidney cells relates to glucose concentration. Human kidney HK-2 cells were infected with different ZIKV strains in presence of normal and high glucose concentrations. Virological assays showed a decrease in viral replication without modifying entry steps (viral binding, internalization, fusion) under high glucose conditions. This decrease replication was associated with a lower virus progeny and increased cell viability when compared to ZIKV-infected HK-2 cells in normal glucose concentration. In conclusion, we showed for the first time that an elevated glucose level influences ZIKV replication level with an effect on kidney cell survival. MDPI 2021-03-02 /pmc/articles/PMC7958337/ /pubmed/33801335 http://dx.doi.org/10.3390/ijms22052495 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reslan, Alawiya
Haddad, Juliano G.
Moukendza Koundi, Liadrine
Desprès, Philippe
Bascands, Jean-Loup
Gadea, Gilles
Zika Virus Growth in Human Kidney Cells Is Restricted by an Elevated Glucose Level
title Zika Virus Growth in Human Kidney Cells Is Restricted by an Elevated Glucose Level
title_full Zika Virus Growth in Human Kidney Cells Is Restricted by an Elevated Glucose Level
title_fullStr Zika Virus Growth in Human Kidney Cells Is Restricted by an Elevated Glucose Level
title_full_unstemmed Zika Virus Growth in Human Kidney Cells Is Restricted by an Elevated Glucose Level
title_short Zika Virus Growth in Human Kidney Cells Is Restricted by an Elevated Glucose Level
title_sort zika virus growth in human kidney cells is restricted by an elevated glucose level
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958337/
https://www.ncbi.nlm.nih.gov/pubmed/33801335
http://dx.doi.org/10.3390/ijms22052495
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