Cargando…
Circulating proteins associated with allergy development in infants—an exploratory analysis
BACKGROUND: Protein profiles that can predict allergy development in children are lacking and the ideal sampling age is unknown. By applying an exploratory proteomics approach in the prospective FARMFLORA birth cohort, we sought to identify previously unknown circulating proteins in early life that...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958444/ https://www.ncbi.nlm.nih.gov/pubmed/33722194 http://dx.doi.org/10.1186/s12014-021-09318-w |
_version_ | 1783664820565835776 |
---|---|
author | Stockfelt, Marit Hong, Mun-Gwan Hesselmar, Bill Adlerberth, Ingegerd Wold, Agnes E. Schwenk, Jochen M. Lundell, Anna-Carin Rudin, Anna |
author_facet | Stockfelt, Marit Hong, Mun-Gwan Hesselmar, Bill Adlerberth, Ingegerd Wold, Agnes E. Schwenk, Jochen M. Lundell, Anna-Carin Rudin, Anna |
author_sort | Stockfelt, Marit |
collection | PubMed |
description | BACKGROUND: Protein profiles that can predict allergy development in children are lacking and the ideal sampling age is unknown. By applying an exploratory proteomics approach in the prospective FARMFLORA birth cohort, we sought to identify previously unknown circulating proteins in early life that associate to protection or risk for development of allergy up to 8 years of age. METHODS: We analyzed plasma prepared from umbilical cord blood (n = 38) and blood collected at 1 month (n = 42), 4 months (n = 39), 18 months (n = 42), 36 months (n = 42) and 8 years (n = 44) of age. We profiled 230 proteins with a multiplexed assay and evaluated the global structure of the data with principal component analysis (PCA). Protein profiles informative to allergic disease at 18 months, 36 months and/or 8 years were evaluated using Lasso logistic regression and random forest. RESULTS: Two clusters emerged in the PCA analysis that separated samples obtained at birth and at 1 month of age from samples obtained later. Differences between the clusters were mostly driven by abundant plasma proteins. For the prediction of allergy, both Lasso logistic regression and random forest were most informative with samples collected at 1 month of age. A Lasso model with 27 proteins together with farm environment differentiated children who remained healthy from those developing allergy. This protein panel was primarily composed of antigen-presenting MHC class I molecules, interleukins and chemokines. CONCLUSION: Sampled at one month of age, circulating proteins that reflect processes of the immune system may predict the development of allergic disease later in childhood. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12014-021-09318-w. |
format | Online Article Text |
id | pubmed-7958444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-79584442021-03-16 Circulating proteins associated with allergy development in infants—an exploratory analysis Stockfelt, Marit Hong, Mun-Gwan Hesselmar, Bill Adlerberth, Ingegerd Wold, Agnes E. Schwenk, Jochen M. Lundell, Anna-Carin Rudin, Anna Clin Proteomics Research BACKGROUND: Protein profiles that can predict allergy development in children are lacking and the ideal sampling age is unknown. By applying an exploratory proteomics approach in the prospective FARMFLORA birth cohort, we sought to identify previously unknown circulating proteins in early life that associate to protection or risk for development of allergy up to 8 years of age. METHODS: We analyzed plasma prepared from umbilical cord blood (n = 38) and blood collected at 1 month (n = 42), 4 months (n = 39), 18 months (n = 42), 36 months (n = 42) and 8 years (n = 44) of age. We profiled 230 proteins with a multiplexed assay and evaluated the global structure of the data with principal component analysis (PCA). Protein profiles informative to allergic disease at 18 months, 36 months and/or 8 years were evaluated using Lasso logistic regression and random forest. RESULTS: Two clusters emerged in the PCA analysis that separated samples obtained at birth and at 1 month of age from samples obtained later. Differences between the clusters were mostly driven by abundant plasma proteins. For the prediction of allergy, both Lasso logistic regression and random forest were most informative with samples collected at 1 month of age. A Lasso model with 27 proteins together with farm environment differentiated children who remained healthy from those developing allergy. This protein panel was primarily composed of antigen-presenting MHC class I molecules, interleukins and chemokines. CONCLUSION: Sampled at one month of age, circulating proteins that reflect processes of the immune system may predict the development of allergic disease later in childhood. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12014-021-09318-w. BioMed Central 2021-03-15 /pmc/articles/PMC7958444/ /pubmed/33722194 http://dx.doi.org/10.1186/s12014-021-09318-w Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Stockfelt, Marit Hong, Mun-Gwan Hesselmar, Bill Adlerberth, Ingegerd Wold, Agnes E. Schwenk, Jochen M. Lundell, Anna-Carin Rudin, Anna Circulating proteins associated with allergy development in infants—an exploratory analysis |
title | Circulating proteins associated with allergy development in infants—an exploratory analysis |
title_full | Circulating proteins associated with allergy development in infants—an exploratory analysis |
title_fullStr | Circulating proteins associated with allergy development in infants—an exploratory analysis |
title_full_unstemmed | Circulating proteins associated with allergy development in infants—an exploratory analysis |
title_short | Circulating proteins associated with allergy development in infants—an exploratory analysis |
title_sort | circulating proteins associated with allergy development in infants—an exploratory analysis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958444/ https://www.ncbi.nlm.nih.gov/pubmed/33722194 http://dx.doi.org/10.1186/s12014-021-09318-w |
work_keys_str_mv | AT stockfeltmarit circulatingproteinsassociatedwithallergydevelopmentininfantsanexploratoryanalysis AT hongmungwan circulatingproteinsassociatedwithallergydevelopmentininfantsanexploratoryanalysis AT hesselmarbill circulatingproteinsassociatedwithallergydevelopmentininfantsanexploratoryanalysis AT adlerberthingegerd circulatingproteinsassociatedwithallergydevelopmentininfantsanexploratoryanalysis AT woldagnese circulatingproteinsassociatedwithallergydevelopmentininfantsanexploratoryanalysis AT schwenkjochenm circulatingproteinsassociatedwithallergydevelopmentininfantsanexploratoryanalysis AT lundellannacarin circulatingproteinsassociatedwithallergydevelopmentininfantsanexploratoryanalysis AT rudinanna circulatingproteinsassociatedwithallergydevelopmentininfantsanexploratoryanalysis |