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Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression
BACKGROUND: Candida tropicalis (C. tropicalis) is an important opportunistic pathogenic Candida species that can cause nosocomial infection. In this study, we analyzed the distribution and drug susceptibility of C. tropicalis and the relationship between ERG11 and UPC2 expression and resistance to a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958445/ https://www.ncbi.nlm.nih.gov/pubmed/33722286 http://dx.doi.org/10.1186/s13756-021-00890-2 |
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author | Wang, Dan An, Na Yang, Yuwei Yang, Xianggui Fan, Yingzi Feng, Jiafu |
author_facet | Wang, Dan An, Na Yang, Yuwei Yang, Xianggui Fan, Yingzi Feng, Jiafu |
author_sort | Wang, Dan |
collection | PubMed |
description | BACKGROUND: Candida tropicalis (C. tropicalis) is an important opportunistic pathogenic Candida species that can cause nosocomial infection. In this study, we analyzed the distribution and drug susceptibility of C. tropicalis and the relationship between ERG11 and UPC2 expression and resistance to azole antifungal agents. METHODS: C. tropicalis was cultured and identified by Sabouraud Agar Medium, CHROM Agar Candida and ATB tests (Bio-Mérieux, France). Total RNA was extracted from the collected strains, and the ERG11 and UPC2 mRNA expression levels were analyzed by quantitative real-time PCR. RESULTS: In total, 2872 clinical isolates of Candida, including 319 strains of C. tropicalis, were analyzed herein; they were mainly obtained from the Departments of Respiratory Medicine and ICU. The strains were predominantly isolated from airway secretion samples, and the detection trend in four years was mainly related to the type of department and specimens. The resistance rates of C. tropicalis to fluconazole, itraconazole and voriconazole had been increasing year by year. The mRNA expression levels of ERG11 and UPC2 in the fluconazole-resistant group were significantly higher than they were in the susceptible group. In addition, there was a significant positive linear correlation between these two genes in the fluconazole-resistant group. CONCLUSIONS: Overexpression of the ERG11 and UPC2 genes in C. tropicalis could increase resistance to azole antifungal drugs. The routine testing for ERG11 and UPC2 in high-risk patients in key departments would provide a theoretical basis for the rational application of azole antifungal drugs. |
format | Online Article Text |
id | pubmed-7958445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-79584452021-03-16 Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression Wang, Dan An, Na Yang, Yuwei Yang, Xianggui Fan, Yingzi Feng, Jiafu Antimicrob Resist Infect Control Research BACKGROUND: Candida tropicalis (C. tropicalis) is an important opportunistic pathogenic Candida species that can cause nosocomial infection. In this study, we analyzed the distribution and drug susceptibility of C. tropicalis and the relationship between ERG11 and UPC2 expression and resistance to azole antifungal agents. METHODS: C. tropicalis was cultured and identified by Sabouraud Agar Medium, CHROM Agar Candida and ATB tests (Bio-Mérieux, France). Total RNA was extracted from the collected strains, and the ERG11 and UPC2 mRNA expression levels were analyzed by quantitative real-time PCR. RESULTS: In total, 2872 clinical isolates of Candida, including 319 strains of C. tropicalis, were analyzed herein; they were mainly obtained from the Departments of Respiratory Medicine and ICU. The strains were predominantly isolated from airway secretion samples, and the detection trend in four years was mainly related to the type of department and specimens. The resistance rates of C. tropicalis to fluconazole, itraconazole and voriconazole had been increasing year by year. The mRNA expression levels of ERG11 and UPC2 in the fluconazole-resistant group were significantly higher than they were in the susceptible group. In addition, there was a significant positive linear correlation between these two genes in the fluconazole-resistant group. CONCLUSIONS: Overexpression of the ERG11 and UPC2 genes in C. tropicalis could increase resistance to azole antifungal drugs. The routine testing for ERG11 and UPC2 in high-risk patients in key departments would provide a theoretical basis for the rational application of azole antifungal drugs. BioMed Central 2021-03-15 /pmc/articles/PMC7958445/ /pubmed/33722286 http://dx.doi.org/10.1186/s13756-021-00890-2 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Wang, Dan An, Na Yang, Yuwei Yang, Xianggui Fan, Yingzi Feng, Jiafu Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression |
title | Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression |
title_full | Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression |
title_fullStr | Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression |
title_full_unstemmed | Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression |
title_short | Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression |
title_sort | candida tropicalis distribution and drug resistance is correlated with erg11 and upc2 expression |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958445/ https://www.ncbi.nlm.nih.gov/pubmed/33722286 http://dx.doi.org/10.1186/s13756-021-00890-2 |
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