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Determination of Trimethylamine N-oxide and Betaine in Serum and Food by Targeted Metabonomics

Trimethylamine N-oxide (TMAO), as a gut-derived metabolite, has been found to be associated with enhanced risk for atherosclerosis and cardiovascular disease. We presented a method for targeted profiling of TMAO and betaine in serum and food samples based on a combination of one-step sample pretreat...

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Autores principales: He, Mingshuai, Yu, Heshui, Lei, Peng, Huang, Shengjie, Ren, Juanning, Fan, Wenjing, Han, Lifeng, Yu, Haiyang, Wang, Yuefei, Ren, Ming, Jiang, Miaomiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958608/
https://www.ncbi.nlm.nih.gov/pubmed/33801417
http://dx.doi.org/10.3390/molecules26051334
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author He, Mingshuai
Yu, Heshui
Lei, Peng
Huang, Shengjie
Ren, Juanning
Fan, Wenjing
Han, Lifeng
Yu, Haiyang
Wang, Yuefei
Ren, Ming
Jiang, Miaomiao
author_facet He, Mingshuai
Yu, Heshui
Lei, Peng
Huang, Shengjie
Ren, Juanning
Fan, Wenjing
Han, Lifeng
Yu, Haiyang
Wang, Yuefei
Ren, Ming
Jiang, Miaomiao
author_sort He, Mingshuai
collection PubMed
description Trimethylamine N-oxide (TMAO), as a gut-derived metabolite, has been found to be associated with enhanced risk for atherosclerosis and cardiovascular disease. We presented a method for targeted profiling of TMAO and betaine in serum and food samples based on a combination of one-step sample pretreatment and proton nuclear magnetic resonance spectroscopy. The key step included a processing of sample preparation using a selective solid-phase extraction column for retention of basic metabolites. Proton signals at δ 3.29 and δ 3.28 were employed to quantify TMAO and betaine, respectively. The developed method was examined with acceptable linear relationship, precision, stability, repeatability, and accuracy. It was successfully applied to detect serum levels of TMAO and betaine in TMAO-fed mice and high-fructose-fed rats and also used to determine the contents of TMAO and betaine in several kinds of food, such as fish, pork, milk, and egg yolk.
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spelling pubmed-79586082021-03-16 Determination of Trimethylamine N-oxide and Betaine in Serum and Food by Targeted Metabonomics He, Mingshuai Yu, Heshui Lei, Peng Huang, Shengjie Ren, Juanning Fan, Wenjing Han, Lifeng Yu, Haiyang Wang, Yuefei Ren, Ming Jiang, Miaomiao Molecules Article Trimethylamine N-oxide (TMAO), as a gut-derived metabolite, has been found to be associated with enhanced risk for atherosclerosis and cardiovascular disease. We presented a method for targeted profiling of TMAO and betaine in serum and food samples based on a combination of one-step sample pretreatment and proton nuclear magnetic resonance spectroscopy. The key step included a processing of sample preparation using a selective solid-phase extraction column for retention of basic metabolites. Proton signals at δ 3.29 and δ 3.28 were employed to quantify TMAO and betaine, respectively. The developed method was examined with acceptable linear relationship, precision, stability, repeatability, and accuracy. It was successfully applied to detect serum levels of TMAO and betaine in TMAO-fed mice and high-fructose-fed rats and also used to determine the contents of TMAO and betaine in several kinds of food, such as fish, pork, milk, and egg yolk. MDPI 2021-03-02 /pmc/articles/PMC7958608/ /pubmed/33801417 http://dx.doi.org/10.3390/molecules26051334 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Mingshuai
Yu, Heshui
Lei, Peng
Huang, Shengjie
Ren, Juanning
Fan, Wenjing
Han, Lifeng
Yu, Haiyang
Wang, Yuefei
Ren, Ming
Jiang, Miaomiao
Determination of Trimethylamine N-oxide and Betaine in Serum and Food by Targeted Metabonomics
title Determination of Trimethylamine N-oxide and Betaine in Serum and Food by Targeted Metabonomics
title_full Determination of Trimethylamine N-oxide and Betaine in Serum and Food by Targeted Metabonomics
title_fullStr Determination of Trimethylamine N-oxide and Betaine in Serum and Food by Targeted Metabonomics
title_full_unstemmed Determination of Trimethylamine N-oxide and Betaine in Serum and Food by Targeted Metabonomics
title_short Determination of Trimethylamine N-oxide and Betaine in Serum and Food by Targeted Metabonomics
title_sort determination of trimethylamine n-oxide and betaine in serum and food by targeted metabonomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958608/
https://www.ncbi.nlm.nih.gov/pubmed/33801417
http://dx.doi.org/10.3390/molecules26051334
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