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Segmental analysis by speckle-tracking echocardiography of the left ventricle response to isoproterenol in male and female mice

We studied by conventional and speckle-tracking echocardiography, the response of the left ventricle (LV) to a three-week continuous infusion of isoproterenol (Iso), a non-specific beta-adrenergic receptor agonist in male and female C57Bl6/J mice. Before and after Iso (30 mg/kg/day), we characterize...

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Autores principales: Walsh-Wilkinson, Elisabeth, Arsenault, Marie, Couet, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958899/
https://www.ncbi.nlm.nih.gov/pubmed/33763310
http://dx.doi.org/10.7717/peerj.11085
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author Walsh-Wilkinson, Elisabeth
Arsenault, Marie
Couet, Jacques
author_facet Walsh-Wilkinson, Elisabeth
Arsenault, Marie
Couet, Jacques
author_sort Walsh-Wilkinson, Elisabeth
collection PubMed
description We studied by conventional and speckle-tracking echocardiography, the response of the left ventricle (LV) to a three-week continuous infusion of isoproterenol (Iso), a non-specific beta-adrenergic receptor agonist in male and female C57Bl6/J mice. Before and after Iso (30 mg/kg/day), we characterized LV morphology and function as well as global and segmental strain. We observed that Iso reduced LV ejection in both male (−8.7%) and female (−14.7%) mice. Several diastolic function parameters were negatively regulated in males and females such as E/A, E/E′, isovolumetric relaxation time. Global longitudinal (GLS) and circumferential (GCS) strains were reduced by Iso in both sexes, GLS by 31% and GCS by about 20%. For the segmental LV analysis, we measured strain, strain rate, reverse strain rate, peak speckle displacement and peak speckle velocity in the parasternal long axis. We observed that radial strain of the LV posterior segments were more severely modulated by Iso than those of the anterior wall in males. In females, on the other hand, both posterior and anterior wall segments were negatively impacted by Iso. Longitudinal strain showed similar results to the radial strain for both sexes. Strain rate, on the other hand, was only moderately changed by Iso. Reverse strain rate measurements (an index of diastolic function) showed that posterior LV segments were negatively regulated by Iso. We then studied the animals 5 and 17 weeks after Iso treatment. Compared to control mice, LV dilation was still present in males. Ejection fraction was decreased in mice of both sex compared to control animals. Diastolic function parameters, on the other hand, were back to normal. Taken together, our study indicates that segmental strain analysis can identify LV regions that are more negatively affected by a cardiotoxic agent such as Iso. In addition, cessation of Iso was not accompanied with a complete restoration of cardiac function after four months.
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spelling pubmed-79588992021-03-23 Segmental analysis by speckle-tracking echocardiography of the left ventricle response to isoproterenol in male and female mice Walsh-Wilkinson, Elisabeth Arsenault, Marie Couet, Jacques PeerJ Biochemistry We studied by conventional and speckle-tracking echocardiography, the response of the left ventricle (LV) to a three-week continuous infusion of isoproterenol (Iso), a non-specific beta-adrenergic receptor agonist in male and female C57Bl6/J mice. Before and after Iso (30 mg/kg/day), we characterized LV morphology and function as well as global and segmental strain. We observed that Iso reduced LV ejection in both male (−8.7%) and female (−14.7%) mice. Several diastolic function parameters were negatively regulated in males and females such as E/A, E/E′, isovolumetric relaxation time. Global longitudinal (GLS) and circumferential (GCS) strains were reduced by Iso in both sexes, GLS by 31% and GCS by about 20%. For the segmental LV analysis, we measured strain, strain rate, reverse strain rate, peak speckle displacement and peak speckle velocity in the parasternal long axis. We observed that radial strain of the LV posterior segments were more severely modulated by Iso than those of the anterior wall in males. In females, on the other hand, both posterior and anterior wall segments were negatively impacted by Iso. Longitudinal strain showed similar results to the radial strain for both sexes. Strain rate, on the other hand, was only moderately changed by Iso. Reverse strain rate measurements (an index of diastolic function) showed that posterior LV segments were negatively regulated by Iso. We then studied the animals 5 and 17 weeks after Iso treatment. Compared to control mice, LV dilation was still present in males. Ejection fraction was decreased in mice of both sex compared to control animals. Diastolic function parameters, on the other hand, were back to normal. Taken together, our study indicates that segmental strain analysis can identify LV regions that are more negatively affected by a cardiotoxic agent such as Iso. In addition, cessation of Iso was not accompanied with a complete restoration of cardiac function after four months. PeerJ Inc. 2021-03-12 /pmc/articles/PMC7958899/ /pubmed/33763310 http://dx.doi.org/10.7717/peerj.11085 Text en ©2021 Walsh-Wilkinson et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Walsh-Wilkinson, Elisabeth
Arsenault, Marie
Couet, Jacques
Segmental analysis by speckle-tracking echocardiography of the left ventricle response to isoproterenol in male and female mice
title Segmental analysis by speckle-tracking echocardiography of the left ventricle response to isoproterenol in male and female mice
title_full Segmental analysis by speckle-tracking echocardiography of the left ventricle response to isoproterenol in male and female mice
title_fullStr Segmental analysis by speckle-tracking echocardiography of the left ventricle response to isoproterenol in male and female mice
title_full_unstemmed Segmental analysis by speckle-tracking echocardiography of the left ventricle response to isoproterenol in male and female mice
title_short Segmental analysis by speckle-tracking echocardiography of the left ventricle response to isoproterenol in male and female mice
title_sort segmental analysis by speckle-tracking echocardiography of the left ventricle response to isoproterenol in male and female mice
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958899/
https://www.ncbi.nlm.nih.gov/pubmed/33763310
http://dx.doi.org/10.7717/peerj.11085
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