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Metabolomic Analysis of Aqueous Humor Identifies Aberrant Amino Acid and Fatty Acid Metabolism in Vogt-Koyanagi-Harada and Behcet’s Disease

To investigate aqueous metabolic profiles in Vogt-Koyanagi-Harada (VKH) and Behcet’s disease (BD), we applied ultra-high-performance liquid chromatography equipped with quadrupole time-of flight mass spectrometry in aqueous humor samples collected from these patients and controls. Metabolite levels...

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Autores principales: Xu, Jing, Su, Guannan, Huang, Xinyue, Chang, Rui, Chen, Zhijun, Ye, Zi, Cao, Qingfeng, Kijlstra, Aize, Yang, Peizeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959366/
https://www.ncbi.nlm.nih.gov/pubmed/33732231
http://dx.doi.org/10.3389/fimmu.2021.587393
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author Xu, Jing
Su, Guannan
Huang, Xinyue
Chang, Rui
Chen, Zhijun
Ye, Zi
Cao, Qingfeng
Kijlstra, Aize
Yang, Peizeng
author_facet Xu, Jing
Su, Guannan
Huang, Xinyue
Chang, Rui
Chen, Zhijun
Ye, Zi
Cao, Qingfeng
Kijlstra, Aize
Yang, Peizeng
author_sort Xu, Jing
collection PubMed
description To investigate aqueous metabolic profiles in Vogt-Koyanagi-Harada (VKH) and Behcet’s disease (BD), we applied ultra-high-performance liquid chromatography equipped with quadrupole time-of flight mass spectrometry in aqueous humor samples collected from these patients and controls. Metabolite levels in these three groups were analyzed by univariate logistic regression. The differential metabolites were subjected to subsequent pathway analysis by MetaboAnalyst. The results showed that both partial-least squares discrimination analysis and hierarchical clustering analysis showed specific aqueous metabolite profiles when comparing VKH, BD, and controls. There were 28 differential metabolites in VKH compared to controls and 29 differential metabolites in BD compared to controls. Amino acids and fatty acids were the two most abundant categories of differential metabolites. Furthermore, pathway enrichment analysis identified several perturbed pathways, including pantothenate and CoA biosynthesis when comparing VKH with the control group, and D-arginine and D-ornithine metabolism and phenylalanine metabolism when comparing BD with the control group. Aminoacyl-tRNA biosynthesis was altered in both VKH and BD when compared to controls. Our findings suggest that amino acids metabolism as well as two fatty acids, palmitic acid and oleic acid, may be involved in the pathogenesis of BD and VKH.
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spelling pubmed-79593662021-03-16 Metabolomic Analysis of Aqueous Humor Identifies Aberrant Amino Acid and Fatty Acid Metabolism in Vogt-Koyanagi-Harada and Behcet’s Disease Xu, Jing Su, Guannan Huang, Xinyue Chang, Rui Chen, Zhijun Ye, Zi Cao, Qingfeng Kijlstra, Aize Yang, Peizeng Front Immunol Immunology To investigate aqueous metabolic profiles in Vogt-Koyanagi-Harada (VKH) and Behcet’s disease (BD), we applied ultra-high-performance liquid chromatography equipped with quadrupole time-of flight mass spectrometry in aqueous humor samples collected from these patients and controls. Metabolite levels in these three groups were analyzed by univariate logistic regression. The differential metabolites were subjected to subsequent pathway analysis by MetaboAnalyst. The results showed that both partial-least squares discrimination analysis and hierarchical clustering analysis showed specific aqueous metabolite profiles when comparing VKH, BD, and controls. There were 28 differential metabolites in VKH compared to controls and 29 differential metabolites in BD compared to controls. Amino acids and fatty acids were the two most abundant categories of differential metabolites. Furthermore, pathway enrichment analysis identified several perturbed pathways, including pantothenate and CoA biosynthesis when comparing VKH with the control group, and D-arginine and D-ornithine metabolism and phenylalanine metabolism when comparing BD with the control group. Aminoacyl-tRNA biosynthesis was altered in both VKH and BD when compared to controls. Our findings suggest that amino acids metabolism as well as two fatty acids, palmitic acid and oleic acid, may be involved in the pathogenesis of BD and VKH. Frontiers Media S.A. 2021-02-22 /pmc/articles/PMC7959366/ /pubmed/33732231 http://dx.doi.org/10.3389/fimmu.2021.587393 Text en Copyright © 2021 Xu, Su, Huang, Chang, Chen, Ye, Cao, Kijlstra and Yang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Xu, Jing
Su, Guannan
Huang, Xinyue
Chang, Rui
Chen, Zhijun
Ye, Zi
Cao, Qingfeng
Kijlstra, Aize
Yang, Peizeng
Metabolomic Analysis of Aqueous Humor Identifies Aberrant Amino Acid and Fatty Acid Metabolism in Vogt-Koyanagi-Harada and Behcet’s Disease
title Metabolomic Analysis of Aqueous Humor Identifies Aberrant Amino Acid and Fatty Acid Metabolism in Vogt-Koyanagi-Harada and Behcet’s Disease
title_full Metabolomic Analysis of Aqueous Humor Identifies Aberrant Amino Acid and Fatty Acid Metabolism in Vogt-Koyanagi-Harada and Behcet’s Disease
title_fullStr Metabolomic Analysis of Aqueous Humor Identifies Aberrant Amino Acid and Fatty Acid Metabolism in Vogt-Koyanagi-Harada and Behcet’s Disease
title_full_unstemmed Metabolomic Analysis of Aqueous Humor Identifies Aberrant Amino Acid and Fatty Acid Metabolism in Vogt-Koyanagi-Harada and Behcet’s Disease
title_short Metabolomic Analysis of Aqueous Humor Identifies Aberrant Amino Acid and Fatty Acid Metabolism in Vogt-Koyanagi-Harada and Behcet’s Disease
title_sort metabolomic analysis of aqueous humor identifies aberrant amino acid and fatty acid metabolism in vogt-koyanagi-harada and behcet’s disease
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959366/
https://www.ncbi.nlm.nih.gov/pubmed/33732231
http://dx.doi.org/10.3389/fimmu.2021.587393
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