Cargando…
Plasmonic probing of the adhesion strength of single microbial cells
Probing the binding between a microbe and surface is critical for understanding biofilm formation processes, developing biosensors, and designing biomaterials, but it remains a challenge. Here, we demonstrate a method to measure the interfacial forces of bacteria attached to the surface. We tracked...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959563/ https://www.ncbi.nlm.nih.gov/pubmed/33060295 http://dx.doi.org/10.1073/pnas.2010136117 |
_version_ | 1783664979264667648 |
---|---|
author | Liu, Yi-Nan Lv, Zhen-Ting Lv, Wen-Li Liu, Xian-Wei |
author_facet | Liu, Yi-Nan Lv, Zhen-Ting Lv, Wen-Li Liu, Xian-Wei |
author_sort | Liu, Yi-Nan |
collection | PubMed |
description | Probing the binding between a microbe and surface is critical for understanding biofilm formation processes, developing biosensors, and designing biomaterials, but it remains a challenge. Here, we demonstrate a method to measure the interfacial forces of bacteria attached to the surface. We tracked the intrinsic fluctuations of individual bacterial cells using an interferometric plasmonic imaging technique. Unlike the existing methods, this approach determined the potential energy profile and quantified the adhesion strength of single cells by analyzing the fluctuations. This method provides insights into biofilm formation and can also serve as a promising platform for investigating biological entity/surface interactions, such as pathogenicity, microbial cell capture and detection, and antimicrobial interface screening. |
format | Online Article Text |
id | pubmed-7959563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-79595632021-03-22 Plasmonic probing of the adhesion strength of single microbial cells Liu, Yi-Nan Lv, Zhen-Ting Lv, Wen-Li Liu, Xian-Wei Proc Natl Acad Sci U S A Physical Sciences Probing the binding between a microbe and surface is critical for understanding biofilm formation processes, developing biosensors, and designing biomaterials, but it remains a challenge. Here, we demonstrate a method to measure the interfacial forces of bacteria attached to the surface. We tracked the intrinsic fluctuations of individual bacterial cells using an interferometric plasmonic imaging technique. Unlike the existing methods, this approach determined the potential energy profile and quantified the adhesion strength of single cells by analyzing the fluctuations. This method provides insights into biofilm formation and can also serve as a promising platform for investigating biological entity/surface interactions, such as pathogenicity, microbial cell capture and detection, and antimicrobial interface screening. National Academy of Sciences 2020-11-03 2020-10-15 /pmc/articles/PMC7959563/ /pubmed/33060295 http://dx.doi.org/10.1073/pnas.2010136117 Text en Copyright © 2020 the Author(s). Published by PNAS. http://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Physical Sciences Liu, Yi-Nan Lv, Zhen-Ting Lv, Wen-Li Liu, Xian-Wei Plasmonic probing of the adhesion strength of single microbial cells |
title | Plasmonic probing of the adhesion strength of single microbial cells |
title_full | Plasmonic probing of the adhesion strength of single microbial cells |
title_fullStr | Plasmonic probing of the adhesion strength of single microbial cells |
title_full_unstemmed | Plasmonic probing of the adhesion strength of single microbial cells |
title_short | Plasmonic probing of the adhesion strength of single microbial cells |
title_sort | plasmonic probing of the adhesion strength of single microbial cells |
topic | Physical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7959563/ https://www.ncbi.nlm.nih.gov/pubmed/33060295 http://dx.doi.org/10.1073/pnas.2010136117 |
work_keys_str_mv | AT liuyinan plasmonicprobingoftheadhesionstrengthofsinglemicrobialcells AT lvzhenting plasmonicprobingoftheadhesionstrengthofsinglemicrobialcells AT lvwenli plasmonicprobingoftheadhesionstrengthofsinglemicrobialcells AT liuxianwei plasmonicprobingoftheadhesionstrengthofsinglemicrobialcells |