Co-fractionation/mass spectrometry to identify protein complexes

Co-fractionation/mass spectrometry (CF/MS) is a flexible and powerful method to detect physical associations of proteins. CF/MS can be applied to any tissue or organism without the need for protein-specific antibodies or epitope tags. Here, we outline two alternate protocols for MS preparation of sa...

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Detalles Bibliográficos
Autores principales: McWhite, Claire D., Papoulas, Ophelia, Drew, Kevin, Dang, Vy, Leggere, Janelle C., Sae-Lee, Wisath, Marcotte, Edward M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960544/
https://www.ncbi.nlm.nih.gov/pubmed/33748783
http://dx.doi.org/10.1016/j.xpro.2021.100370
Descripción
Sumario:Co-fractionation/mass spectrometry (CF/MS) is a flexible and powerful method to detect physical associations of proteins. CF/MS can be applied to any tissue or organism without the need for protein-specific antibodies or epitope tags. Here, we outline two alternate protocols for MS preparation of samples (containing low or high salt) and a computational pipeline (cfmsflow) that together allow the successful application of this approach. These protocols are based on CF/MS of over 16 diverse organisms including plants and animals. For complete details on the use and execution of this protocol, please refer to McWhite et al. (2020).

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