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Isolation and Characterization of a Porcine Transmissible Gastroenteritis Coronavirus in Northeast China
Transmissible gastroenteritis virus (TGEV) is a coronavirus (CoV) that is a major pathogenity of viral enteritis and diarrhea in suckling piglets, causing high morbidity and mortality. In this study, a TGEV strain HQ2016 was isolated from northeast China and characterized its genome sequence and pat...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960647/ https://www.ncbi.nlm.nih.gov/pubmed/33738304 http://dx.doi.org/10.3389/fvets.2021.611721 |
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author | Yuan, Dongwei Yan, Zihan Li, Mingyue Wang, Yi Su, Mingjun Sun, Dongbo |
author_facet | Yuan, Dongwei Yan, Zihan Li, Mingyue Wang, Yi Su, Mingjun Sun, Dongbo |
author_sort | Yuan, Dongwei |
collection | PubMed |
description | Transmissible gastroenteritis virus (TGEV) is a coronavirus (CoV) that is a major pathogenity of viral enteritis and diarrhea in suckling piglets, causing high morbidity and mortality. In this study, a TGEV strain HQ2016 was isolated from northeast China and characterized its genome sequence and pathogenicity. The phylogenetic analysis indicated that the TGEV HQ2016 strain was more similar to the TGEV Purdue cluster than to the Miller cluster. Both recombination and phylogenetic analysis based on each structural and non-structural gene revealed no recombination event in the HQ2016 strain. Experimental infection study using colostrum-deprived newborn piglets successfully showed that the HQ2016 can cause clinical symptoms including anorexia and yellow-to-whitish watery diarrhea, which are characteristics of TGE, in the inoculated piglets 48 h post-inoculation. These results provide valuable information about the evolution of the porcine CoVs. |
format | Online Article Text |
id | pubmed-7960647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79606472021-03-17 Isolation and Characterization of a Porcine Transmissible Gastroenteritis Coronavirus in Northeast China Yuan, Dongwei Yan, Zihan Li, Mingyue Wang, Yi Su, Mingjun Sun, Dongbo Front Vet Sci Veterinary Science Transmissible gastroenteritis virus (TGEV) is a coronavirus (CoV) that is a major pathogenity of viral enteritis and diarrhea in suckling piglets, causing high morbidity and mortality. In this study, a TGEV strain HQ2016 was isolated from northeast China and characterized its genome sequence and pathogenicity. The phylogenetic analysis indicated that the TGEV HQ2016 strain was more similar to the TGEV Purdue cluster than to the Miller cluster. Both recombination and phylogenetic analysis based on each structural and non-structural gene revealed no recombination event in the HQ2016 strain. Experimental infection study using colostrum-deprived newborn piglets successfully showed that the HQ2016 can cause clinical symptoms including anorexia and yellow-to-whitish watery diarrhea, which are characteristics of TGE, in the inoculated piglets 48 h post-inoculation. These results provide valuable information about the evolution of the porcine CoVs. Frontiers Media S.A. 2021-03-02 /pmc/articles/PMC7960647/ /pubmed/33738304 http://dx.doi.org/10.3389/fvets.2021.611721 Text en Copyright © 2021 Yuan, Yan, Li, Wang, Su and Sun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Yuan, Dongwei Yan, Zihan Li, Mingyue Wang, Yi Su, Mingjun Sun, Dongbo Isolation and Characterization of a Porcine Transmissible Gastroenteritis Coronavirus in Northeast China |
title | Isolation and Characterization of a Porcine Transmissible Gastroenteritis Coronavirus in Northeast China |
title_full | Isolation and Characterization of a Porcine Transmissible Gastroenteritis Coronavirus in Northeast China |
title_fullStr | Isolation and Characterization of a Porcine Transmissible Gastroenteritis Coronavirus in Northeast China |
title_full_unstemmed | Isolation and Characterization of a Porcine Transmissible Gastroenteritis Coronavirus in Northeast China |
title_short | Isolation and Characterization of a Porcine Transmissible Gastroenteritis Coronavirus in Northeast China |
title_sort | isolation and characterization of a porcine transmissible gastroenteritis coronavirus in northeast china |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960647/ https://www.ncbi.nlm.nih.gov/pubmed/33738304 http://dx.doi.org/10.3389/fvets.2021.611721 |
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