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Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety
Cadmium (Cd) contamination of rice is a serious food safety issue that has recently been gaining significant public attention. Therefore, reduction of Cd accumulation in rice grains is an important objective of rice breeding. The use of favourable alleles of Cd accumulating genes using marker-assist...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961068/ https://www.ncbi.nlm.nih.gov/pubmed/33723281 http://dx.doi.org/10.1038/s41598-021-85324-0 |
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author | Wang, Kai Yan, Tian-ze Xu, Shi-long Yan, Xu Zhou, Qun-feng Zhao, Xin-hui Li, Yan-feng Wu, Zhong-xiu Qin, Peng Fu, Chen-jian Fu, Jun Zhou, Yan-biao Yang, Yuan-zhu |
author_facet | Wang, Kai Yan, Tian-ze Xu, Shi-long Yan, Xu Zhou, Qun-feng Zhao, Xin-hui Li, Yan-feng Wu, Zhong-xiu Qin, Peng Fu, Chen-jian Fu, Jun Zhou, Yan-biao Yang, Yuan-zhu |
author_sort | Wang, Kai |
collection | PubMed |
description | Cadmium (Cd) contamination of rice is a serious food safety issue that has recently been gaining significant public attention. Therefore, reduction of Cd accumulation in rice grains is an important objective of rice breeding. The use of favourable alleles of Cd accumulating genes using marker-assisted selection (MAS) is theoretically feasible. In this study, we validated a segment covering OsHMA3-OsNramp5-OsNramp1 on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety. The OsHMA3-OsNramp5-OsNramp1(jap) haplotype significantly decreased grain Cd concentration in middle-season indica genetic background. The improved 9311 carrying the OsHMA3-OsNramp5-OsNramp1(jap) haplotype with recurrent parent genome recovery of up to 91.6% resulted in approximately 31.8% decrease in Cd accumulation in the grain and with no penalty on yield. There is a genetic linkage-drag between OsHMA3-OsNramp5-OsNramp1 (jap) and the gene conditioning heading to days (HTD) in the early-season indica genetic background. Because the OsHMA3-OsNramp5-OsNramp1-Ghd7(jap) haplotype significantly increases grain Cd concentration and prolongs growth duration, the linkage-drag between OsHMA3-OsNramp5-OsNramp1 and Ghd7 should be broken down by large segregating populations or gene editing. A novel allele of OsHMA3 was identified from a wide-compatibility japonica cultivar, the expression differences of OsNramp1 and OsNramp5 in roots might contribute the Cd accumulating variation between japonica and indica variety. |
format | Online Article Text |
id | pubmed-7961068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79610682021-03-19 Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety Wang, Kai Yan, Tian-ze Xu, Shi-long Yan, Xu Zhou, Qun-feng Zhao, Xin-hui Li, Yan-feng Wu, Zhong-xiu Qin, Peng Fu, Chen-jian Fu, Jun Zhou, Yan-biao Yang, Yuan-zhu Sci Rep Article Cadmium (Cd) contamination of rice is a serious food safety issue that has recently been gaining significant public attention. Therefore, reduction of Cd accumulation in rice grains is an important objective of rice breeding. The use of favourable alleles of Cd accumulating genes using marker-assisted selection (MAS) is theoretically feasible. In this study, we validated a segment covering OsHMA3-OsNramp5-OsNramp1 on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety. The OsHMA3-OsNramp5-OsNramp1(jap) haplotype significantly decreased grain Cd concentration in middle-season indica genetic background. The improved 9311 carrying the OsHMA3-OsNramp5-OsNramp1(jap) haplotype with recurrent parent genome recovery of up to 91.6% resulted in approximately 31.8% decrease in Cd accumulation in the grain and with no penalty on yield. There is a genetic linkage-drag between OsHMA3-OsNramp5-OsNramp1 (jap) and the gene conditioning heading to days (HTD) in the early-season indica genetic background. Because the OsHMA3-OsNramp5-OsNramp1-Ghd7(jap) haplotype significantly increases grain Cd concentration and prolongs growth duration, the linkage-drag between OsHMA3-OsNramp5-OsNramp1 and Ghd7 should be broken down by large segregating populations or gene editing. A novel allele of OsHMA3 was identified from a wide-compatibility japonica cultivar, the expression differences of OsNramp1 and OsNramp5 in roots might contribute the Cd accumulating variation between japonica and indica variety. Nature Publishing Group UK 2021-03-15 /pmc/articles/PMC7961068/ /pubmed/33723281 http://dx.doi.org/10.1038/s41598-021-85324-0 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wang, Kai Yan, Tian-ze Xu, Shi-long Yan, Xu Zhou, Qun-feng Zhao, Xin-hui Li, Yan-feng Wu, Zhong-xiu Qin, Peng Fu, Chen-jian Fu, Jun Zhou, Yan-biao Yang, Yuan-zhu Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety |
title | Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety |
title_full | Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety |
title_fullStr | Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety |
title_full_unstemmed | Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety |
title_short | Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety |
title_sort | validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961068/ https://www.ncbi.nlm.nih.gov/pubmed/33723281 http://dx.doi.org/10.1038/s41598-021-85324-0 |
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