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Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru

The widespread and poorly regulated use of antibiotics in animal production in low- and middle-income countries (LMICs) is increasingly associated with the emergence and dissemination of antibiotic resistance genes (ARGs) in retail animal products. Here, we compared Escherichia coli from chickens an...

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Autores principales: Murray, Matthew, Salvatierra, Guillermo, Dávila-Barclay, Alejandra, Ayzanoa, Brenda, Castillo-Vilcahuaman, Camila, Huang, Michelle, Pajuelo, Mónica J., Lescano, Andrés G., Cabrera, Lilia, Calderón, Maritza, Berg, Douglas E., Gilman, Robert H., Tsukayama, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961087/
https://www.ncbi.nlm.nih.gov/pubmed/33737922
http://dx.doi.org/10.3389/fmicb.2021.635871
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author Murray, Matthew
Salvatierra, Guillermo
Dávila-Barclay, Alejandra
Ayzanoa, Brenda
Castillo-Vilcahuaman, Camila
Huang, Michelle
Pajuelo, Mónica J.
Lescano, Andrés G.
Cabrera, Lilia
Calderón, Maritza
Berg, Douglas E.
Gilman, Robert H.
Tsukayama, Pablo
author_facet Murray, Matthew
Salvatierra, Guillermo
Dávila-Barclay, Alejandra
Ayzanoa, Brenda
Castillo-Vilcahuaman, Camila
Huang, Michelle
Pajuelo, Mónica J.
Lescano, Andrés G.
Cabrera, Lilia
Calderón, Maritza
Berg, Douglas E.
Gilman, Robert H.
Tsukayama, Pablo
author_sort Murray, Matthew
collection PubMed
description The widespread and poorly regulated use of antibiotics in animal production in low- and middle-income countries (LMICs) is increasingly associated with the emergence and dissemination of antibiotic resistance genes (ARGs) in retail animal products. Here, we compared Escherichia coli from chickens and humans with varying levels of exposure to chicken meat in a low-income community in the southern outskirts of Lima, Peru. We hypothesize that current practices in local poultry production result in highly resistant commensal bacteria in chickens that can potentially colonize the human gut. E. coli was isolated from cloacal swabs of non-organic (n = 41) and organic chickens (n = 20), as well as from stools of market chicken vendors (n = 23), non-vendors (n = 48), and babies (n = 60). 315 E. coli isolates from humans (n = 150) and chickens (n = 165) were identified, with chickens showing higher rates of multidrug-resistant and extended-spectrum beta-lactamase phenotypes. Non-organic chicken isolates were more resistant to most antibiotics tested than human isolates, while organic chicken isolates were susceptible to most antibiotics. Whole-genome sequencing of 118 isolates identified shared phylogroups between human and animal populations and 604 ARG hits across genomes. Resistance to florfenicol (an antibiotic commonly used as a growth promoter in poultry but not approved for human use) was higher in chicken vendors compared to other human groups. Isolates from non-organic chickens contained genes conferring resistance to clinically relevant antibiotics, including mcr-1 for colistin resistance, blaCTX-M ESBLs, and blaKPC-3 carbapenemase. Our findings suggest that E. coli strains from market chickens are a potential source of ARGs that can be transmitted to human commensals.
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spelling pubmed-79610872021-03-17 Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru Murray, Matthew Salvatierra, Guillermo Dávila-Barclay, Alejandra Ayzanoa, Brenda Castillo-Vilcahuaman, Camila Huang, Michelle Pajuelo, Mónica J. Lescano, Andrés G. Cabrera, Lilia Calderón, Maritza Berg, Douglas E. Gilman, Robert H. Tsukayama, Pablo Front Microbiol Microbiology The widespread and poorly regulated use of antibiotics in animal production in low- and middle-income countries (LMICs) is increasingly associated with the emergence and dissemination of antibiotic resistance genes (ARGs) in retail animal products. Here, we compared Escherichia coli from chickens and humans with varying levels of exposure to chicken meat in a low-income community in the southern outskirts of Lima, Peru. We hypothesize that current practices in local poultry production result in highly resistant commensal bacteria in chickens that can potentially colonize the human gut. E. coli was isolated from cloacal swabs of non-organic (n = 41) and organic chickens (n = 20), as well as from stools of market chicken vendors (n = 23), non-vendors (n = 48), and babies (n = 60). 315 E. coli isolates from humans (n = 150) and chickens (n = 165) were identified, with chickens showing higher rates of multidrug-resistant and extended-spectrum beta-lactamase phenotypes. Non-organic chicken isolates were more resistant to most antibiotics tested than human isolates, while organic chicken isolates were susceptible to most antibiotics. Whole-genome sequencing of 118 isolates identified shared phylogroups between human and animal populations and 604 ARG hits across genomes. Resistance to florfenicol (an antibiotic commonly used as a growth promoter in poultry but not approved for human use) was higher in chicken vendors compared to other human groups. Isolates from non-organic chickens contained genes conferring resistance to clinically relevant antibiotics, including mcr-1 for colistin resistance, blaCTX-M ESBLs, and blaKPC-3 carbapenemase. Our findings suggest that E. coli strains from market chickens are a potential source of ARGs that can be transmitted to human commensals. Frontiers Media S.A. 2021-03-02 /pmc/articles/PMC7961087/ /pubmed/33737922 http://dx.doi.org/10.3389/fmicb.2021.635871 Text en Copyright © 2021 Murray, Salvatierra, Dávila-Barclay, Ayzanoa, Castillo-Vilcahuaman, Huang, Pajuelo, Lescano, Cabrera, Calderón, Berg, Gilman and Tsukayama. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Murray, Matthew
Salvatierra, Guillermo
Dávila-Barclay, Alejandra
Ayzanoa, Brenda
Castillo-Vilcahuaman, Camila
Huang, Michelle
Pajuelo, Mónica J.
Lescano, Andrés G.
Cabrera, Lilia
Calderón, Maritza
Berg, Douglas E.
Gilman, Robert H.
Tsukayama, Pablo
Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru
title Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru
title_full Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru
title_fullStr Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru
title_full_unstemmed Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru
title_short Market Chickens as a Source of Antibiotic-Resistant Escherichia coli in a Peri-Urban Community in Lima, Peru
title_sort market chickens as a source of antibiotic-resistant escherichia coli in a peri-urban community in lima, peru
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961087/
https://www.ncbi.nlm.nih.gov/pubmed/33737922
http://dx.doi.org/10.3389/fmicb.2021.635871
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