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Comparison of different sequencing strategies for assembling chromosome-level genomes of extremophiles with variable GC content
In this study, six bacterial isolates with variable GC, including Escherichia coli as mesophilic reference strain, were selected to compare hybrid assembly strategies based on next-generation sequencing (NGS) of short reads, single-tube long-fragment reads (stLFR) sequencing, and Oxford Nanopore Tec...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961107/ https://www.ncbi.nlm.nih.gov/pubmed/33748707 http://dx.doi.org/10.1016/j.isci.2021.102219 |