Comparison of different sequencing strategies for assembling chromosome-level genomes of extremophiles with variable GC content

In this study, six bacterial isolates with variable GC, including Escherichia coli as mesophilic reference strain, were selected to compare hybrid assembly strategies based on next-generation sequencing (NGS) of short reads, single-tube long-fragment reads (stLFR) sequencing, and Oxford Nanopore Tec...

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Detalles Bibliográficos
Autores principales: Zhang, Zhidong, Liu, Guilin, Chen, Yao, Xue, Weizhen, Ji, Qianyue, Xu, Qiwu, Zhang, He, Fan, Guangyi, Huang, He, Jiang, Ling, Chen, Jianwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961107/
https://www.ncbi.nlm.nih.gov/pubmed/33748707
http://dx.doi.org/10.1016/j.isci.2021.102219

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