Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells

Plant polyphenol gossypol has anticancer activities. This may increase cottonseed value by using gossypol as a health intervention agent. It is necessary to understand its molecular mechanisms before human consumption. The aim was to uncover the effects of gossypol on cell viability and gene express...

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Autores principales: Cao, Heping, Sethumadhavan, Kandan, Cao, Fangping, Wang, Thomas T. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961146/
https://www.ncbi.nlm.nih.gov/pubmed/33723275
http://dx.doi.org/10.1038/s41598-021-84970-8
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author Cao, Heping
Sethumadhavan, Kandan
Cao, Fangping
Wang, Thomas T. Y.
author_facet Cao, Heping
Sethumadhavan, Kandan
Cao, Fangping
Wang, Thomas T. Y.
author_sort Cao, Heping
collection PubMed
description Plant polyphenol gossypol has anticancer activities. This may increase cottonseed value by using gossypol as a health intervention agent. It is necessary to understand its molecular mechanisms before human consumption. The aim was to uncover the effects of gossypol on cell viability and gene expression in cancer cells. In this study, human colon cancer cells (COLO 225) were treated with gossypol. MTT assay showed significant inhibitory effect under high concentration and longtime treatment. We analyzed the expression of 55 genes at the mRNA level in the cells; many of them are regulated by gossypol or ZFP36/TTP in cancer cells. BCL2 mRNA was the most stable among the 55 mRNAs analyzed in human colon cancer cells. GAPDH and RPL32 mRNAs were not good qPCR references for the colon cancer cells. Gossypol decreased the mRNA levels of DGAT, GLUT, TTP, IL families and a number of previously reported genes. In particular, gossypol suppressed the expression of genes coding for CLAUDIN1, ELK1, FAS, GAPDH, IL2, IL8 and ZFAND5 mRNAs, but enhanced the expression of the gene coding for GLUT3 mRNA. The results showed that gossypol inhibited cell survival with decreased expression of a number of genes in the colon cancer cells.
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spelling pubmed-79611462021-03-19 Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells Cao, Heping Sethumadhavan, Kandan Cao, Fangping Wang, Thomas T. Y. Sci Rep Article Plant polyphenol gossypol has anticancer activities. This may increase cottonseed value by using gossypol as a health intervention agent. It is necessary to understand its molecular mechanisms before human consumption. The aim was to uncover the effects of gossypol on cell viability and gene expression in cancer cells. In this study, human colon cancer cells (COLO 225) were treated with gossypol. MTT assay showed significant inhibitory effect under high concentration and longtime treatment. We analyzed the expression of 55 genes at the mRNA level in the cells; many of them are regulated by gossypol or ZFP36/TTP in cancer cells. BCL2 mRNA was the most stable among the 55 mRNAs analyzed in human colon cancer cells. GAPDH and RPL32 mRNAs were not good qPCR references for the colon cancer cells. Gossypol decreased the mRNA levels of DGAT, GLUT, TTP, IL families and a number of previously reported genes. In particular, gossypol suppressed the expression of genes coding for CLAUDIN1, ELK1, FAS, GAPDH, IL2, IL8 and ZFAND5 mRNAs, but enhanced the expression of the gene coding for GLUT3 mRNA. The results showed that gossypol inhibited cell survival with decreased expression of a number of genes in the colon cancer cells. Nature Publishing Group UK 2021-03-15 /pmc/articles/PMC7961146/ /pubmed/33723275 http://dx.doi.org/10.1038/s41598-021-84970-8 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Cao, Heping
Sethumadhavan, Kandan
Cao, Fangping
Wang, Thomas T. Y.
Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title_full Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title_fullStr Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title_full_unstemmed Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title_short Gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
title_sort gossypol decreased cell viability and down-regulated the expression of a number of genes in human colon cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961146/
https://www.ncbi.nlm.nih.gov/pubmed/33723275
http://dx.doi.org/10.1038/s41598-021-84970-8
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