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Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug

Purpose: This study aimed to evaluate the biological and mechanical properties of the poly(methyl methacrylate) (PMMA) denture base material as a vehicle incorporating novel hydroxyapatite nanoparticles (HA-NP) loaded with metronidazole (MZ) drug. Methods: HA-NP was prepared via wet-chemical-method,...

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Autores principales: Elboraey, Asmaa Nabil, Abo-Almaged, Hanan Hassan, El-Ashmawy, Ahmed Abd El-Rahman, Abdou, Aya Rashad, Moussa, Amani Ramadan, Emara, Laila Hassanian, El-Masry, Hossam Mohammed, El Bassyouni, Gehan El-Tabie, Ramzy, Magda Ismail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961227/
https://www.ncbi.nlm.nih.gov/pubmed/33747855
http://dx.doi.org/10.34172/apb.2021.009
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author Elboraey, Asmaa Nabil
Abo-Almaged, Hanan Hassan
El-Ashmawy, Ahmed Abd El-Rahman
Abdou, Aya Rashad
Moussa, Amani Ramadan
Emara, Laila Hassanian
El-Masry, Hossam Mohammed
El Bassyouni, Gehan El-Tabie
Ramzy, Magda Ismail
author_facet Elboraey, Asmaa Nabil
Abo-Almaged, Hanan Hassan
El-Ashmawy, Ahmed Abd El-Rahman
Abdou, Aya Rashad
Moussa, Amani Ramadan
Emara, Laila Hassanian
El-Masry, Hossam Mohammed
El Bassyouni, Gehan El-Tabie
Ramzy, Magda Ismail
author_sort Elboraey, Asmaa Nabil
collection PubMed
description Purpose: This study aimed to evaluate the biological and mechanical properties of the poly(methyl methacrylate) (PMMA) denture base material as a vehicle incorporating novel hydroxyapatite nanoparticles (HA-NP) loaded with metronidazole (MZ) drug. Methods: HA-NP was prepared via wet-chemical-method, characterized by XRD, SEM/EDX, TEM, Fourier-transform infrared spectroscopy (FTIR), as well as the measurement of surface area and pore-size distribution. Four drug delivery formulas were prepared in the form of discs (10 x 2 mm) as follows: F1 (MZ/ HA-NP/PMMA), F2 (HA-NP/ PMMA), F3 (control-PMMA) and F4 (MZ/PMMA). Characterization of all formulas was performed using differential scanning calorimetry (DSC) and FTIR. MZ release rate, antimicrobial properties against three oral pathogens, cytotoxicity (MTT assay) and surface micro-hardness were also assessed. Statistical analysis of data was performed using one-way ANOVA test (P < 0.05). Results: DSC thermograms showed compatibility among MZ, HA-NP and PMMA along with physical stability over 6 months storage period at room temperature. FTIR spectroscopy proved the absence of any possible chemical interaction with MZ. MZ-HA-NP/PMMA formula showed relatively better drug release compared to MZ-PMMA. Both formulas showed statistically significant antimicrobial potentials against two microbial strains. MTT demonstrated reduction in cell cytotoxicity after 96 hours with the least value for HA-NP. Surface micro-hardness revealed non-significant reduction compared with the control PMMA. Conclusion: A novel biocompatible drug nanocarrier (HA-NP) was developed and incorporated in PMMA denture base material as a vehicle to allow prolonged sustained drug release to manage oral infections.
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spelling pubmed-79612272021-03-19 Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug Elboraey, Asmaa Nabil Abo-Almaged, Hanan Hassan El-Ashmawy, Ahmed Abd El-Rahman Abdou, Aya Rashad Moussa, Amani Ramadan Emara, Laila Hassanian El-Masry, Hossam Mohammed El Bassyouni, Gehan El-Tabie Ramzy, Magda Ismail Adv Pharm Bull Research Article Purpose: This study aimed to evaluate the biological and mechanical properties of the poly(methyl methacrylate) (PMMA) denture base material as a vehicle incorporating novel hydroxyapatite nanoparticles (HA-NP) loaded with metronidazole (MZ) drug. Methods: HA-NP was prepared via wet-chemical-method, characterized by XRD, SEM/EDX, TEM, Fourier-transform infrared spectroscopy (FTIR), as well as the measurement of surface area and pore-size distribution. Four drug delivery formulas were prepared in the form of discs (10 x 2 mm) as follows: F1 (MZ/ HA-NP/PMMA), F2 (HA-NP/ PMMA), F3 (control-PMMA) and F4 (MZ/PMMA). Characterization of all formulas was performed using differential scanning calorimetry (DSC) and FTIR. MZ release rate, antimicrobial properties against three oral pathogens, cytotoxicity (MTT assay) and surface micro-hardness were also assessed. Statistical analysis of data was performed using one-way ANOVA test (P < 0.05). Results: DSC thermograms showed compatibility among MZ, HA-NP and PMMA along with physical stability over 6 months storage period at room temperature. FTIR spectroscopy proved the absence of any possible chemical interaction with MZ. MZ-HA-NP/PMMA formula showed relatively better drug release compared to MZ-PMMA. Both formulas showed statistically significant antimicrobial potentials against two microbial strains. MTT demonstrated reduction in cell cytotoxicity after 96 hours with the least value for HA-NP. Surface micro-hardness revealed non-significant reduction compared with the control PMMA. Conclusion: A novel biocompatible drug nanocarrier (HA-NP) was developed and incorporated in PMMA denture base material as a vehicle to allow prolonged sustained drug release to manage oral infections. Tabriz University of Medical Sciences 2021-01 2020-11-07 /pmc/articles/PMC7961227/ /pubmed/33747855 http://dx.doi.org/10.34172/apb.2021.009 Text en © 2021 The Authors. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers.
spellingShingle Research Article
Elboraey, Asmaa Nabil
Abo-Almaged, Hanan Hassan
El-Ashmawy, Ahmed Abd El-Rahman
Abdou, Aya Rashad
Moussa, Amani Ramadan
Emara, Laila Hassanian
El-Masry, Hossam Mohammed
El Bassyouni, Gehan El-Tabie
Ramzy, Magda Ismail
Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug
title Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug
title_full Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug
title_fullStr Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug
title_full_unstemmed Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug
title_short Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug
title_sort biological and mechanical properties of denture base material as a vehicle for novel hydroxyapatite nanoparticles loaded with drug
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961227/
https://www.ncbi.nlm.nih.gov/pubmed/33747855
http://dx.doi.org/10.34172/apb.2021.009
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