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Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug
Purpose: This study aimed to evaluate the biological and mechanical properties of the poly(methyl methacrylate) (PMMA) denture base material as a vehicle incorporating novel hydroxyapatite nanoparticles (HA-NP) loaded with metronidazole (MZ) drug. Methods: HA-NP was prepared via wet-chemical-method,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tabriz University of Medical Sciences
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961227/ https://www.ncbi.nlm.nih.gov/pubmed/33747855 http://dx.doi.org/10.34172/apb.2021.009 |
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author | Elboraey, Asmaa Nabil Abo-Almaged, Hanan Hassan El-Ashmawy, Ahmed Abd El-Rahman Abdou, Aya Rashad Moussa, Amani Ramadan Emara, Laila Hassanian El-Masry, Hossam Mohammed El Bassyouni, Gehan El-Tabie Ramzy, Magda Ismail |
author_facet | Elboraey, Asmaa Nabil Abo-Almaged, Hanan Hassan El-Ashmawy, Ahmed Abd El-Rahman Abdou, Aya Rashad Moussa, Amani Ramadan Emara, Laila Hassanian El-Masry, Hossam Mohammed El Bassyouni, Gehan El-Tabie Ramzy, Magda Ismail |
author_sort | Elboraey, Asmaa Nabil |
collection | PubMed |
description | Purpose: This study aimed to evaluate the biological and mechanical properties of the poly(methyl methacrylate) (PMMA) denture base material as a vehicle incorporating novel hydroxyapatite nanoparticles (HA-NP) loaded with metronidazole (MZ) drug. Methods: HA-NP was prepared via wet-chemical-method, characterized by XRD, SEM/EDX, TEM, Fourier-transform infrared spectroscopy (FTIR), as well as the measurement of surface area and pore-size distribution. Four drug delivery formulas were prepared in the form of discs (10 x 2 mm) as follows: F1 (MZ/ HA-NP/PMMA), F2 (HA-NP/ PMMA), F3 (control-PMMA) and F4 (MZ/PMMA). Characterization of all formulas was performed using differential scanning calorimetry (DSC) and FTIR. MZ release rate, antimicrobial properties against three oral pathogens, cytotoxicity (MTT assay) and surface micro-hardness were also assessed. Statistical analysis of data was performed using one-way ANOVA test (P < 0.05). Results: DSC thermograms showed compatibility among MZ, HA-NP and PMMA along with physical stability over 6 months storage period at room temperature. FTIR spectroscopy proved the absence of any possible chemical interaction with MZ. MZ-HA-NP/PMMA formula showed relatively better drug release compared to MZ-PMMA. Both formulas showed statistically significant antimicrobial potentials against two microbial strains. MTT demonstrated reduction in cell cytotoxicity after 96 hours with the least value for HA-NP. Surface micro-hardness revealed non-significant reduction compared with the control PMMA. Conclusion: A novel biocompatible drug nanocarrier (HA-NP) was developed and incorporated in PMMA denture base material as a vehicle to allow prolonged sustained drug release to manage oral infections. |
format | Online Article Text |
id | pubmed-7961227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Tabriz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-79612272021-03-19 Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug Elboraey, Asmaa Nabil Abo-Almaged, Hanan Hassan El-Ashmawy, Ahmed Abd El-Rahman Abdou, Aya Rashad Moussa, Amani Ramadan Emara, Laila Hassanian El-Masry, Hossam Mohammed El Bassyouni, Gehan El-Tabie Ramzy, Magda Ismail Adv Pharm Bull Research Article Purpose: This study aimed to evaluate the biological and mechanical properties of the poly(methyl methacrylate) (PMMA) denture base material as a vehicle incorporating novel hydroxyapatite nanoparticles (HA-NP) loaded with metronidazole (MZ) drug. Methods: HA-NP was prepared via wet-chemical-method, characterized by XRD, SEM/EDX, TEM, Fourier-transform infrared spectroscopy (FTIR), as well as the measurement of surface area and pore-size distribution. Four drug delivery formulas were prepared in the form of discs (10 x 2 mm) as follows: F1 (MZ/ HA-NP/PMMA), F2 (HA-NP/ PMMA), F3 (control-PMMA) and F4 (MZ/PMMA). Characterization of all formulas was performed using differential scanning calorimetry (DSC) and FTIR. MZ release rate, antimicrobial properties against three oral pathogens, cytotoxicity (MTT assay) and surface micro-hardness were also assessed. Statistical analysis of data was performed using one-way ANOVA test (P < 0.05). Results: DSC thermograms showed compatibility among MZ, HA-NP and PMMA along with physical stability over 6 months storage period at room temperature. FTIR spectroscopy proved the absence of any possible chemical interaction with MZ. MZ-HA-NP/PMMA formula showed relatively better drug release compared to MZ-PMMA. Both formulas showed statistically significant antimicrobial potentials against two microbial strains. MTT demonstrated reduction in cell cytotoxicity after 96 hours with the least value for HA-NP. Surface micro-hardness revealed non-significant reduction compared with the control PMMA. Conclusion: A novel biocompatible drug nanocarrier (HA-NP) was developed and incorporated in PMMA denture base material as a vehicle to allow prolonged sustained drug release to manage oral infections. Tabriz University of Medical Sciences 2021-01 2020-11-07 /pmc/articles/PMC7961227/ /pubmed/33747855 http://dx.doi.org/10.34172/apb.2021.009 Text en © 2021 The Authors. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers. |
spellingShingle | Research Article Elboraey, Asmaa Nabil Abo-Almaged, Hanan Hassan El-Ashmawy, Ahmed Abd El-Rahman Abdou, Aya Rashad Moussa, Amani Ramadan Emara, Laila Hassanian El-Masry, Hossam Mohammed El Bassyouni, Gehan El-Tabie Ramzy, Magda Ismail Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug |
title | Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug |
title_full | Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug |
title_fullStr | Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug |
title_full_unstemmed | Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug |
title_short | Biological and Mechanical Properties of Denture Base Material as a Vehicle for Novel Hydroxyapatite Nanoparticles Loaded with Drug |
title_sort | biological and mechanical properties of denture base material as a vehicle for novel hydroxyapatite nanoparticles loaded with drug |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961227/ https://www.ncbi.nlm.nih.gov/pubmed/33747855 http://dx.doi.org/10.34172/apb.2021.009 |
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