Cargando…
Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure
Gut microbiota-derived metabolites, in particular short chain fatty acids (SCFAs) and their receptors, are linked to hypertension. Fructose and antibiotics are commonly used worldwide, and they have a negative impact on the gut microbiota. Our previous study revealed that maternal high-fructose (HF)...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961901/ https://www.ncbi.nlm.nih.gov/pubmed/33800916 http://dx.doi.org/10.3390/ijms22052674 |
_version_ | 1783665359768780800 |
---|---|
author | Hsu, Chien-Ning Chan, Julie Y. H. Wu, Kay L. H. Yu, Hong-Ren Lee, Wei-Chia Hou, Chih-Yao Tain, You-Lin |
author_facet | Hsu, Chien-Ning Chan, Julie Y. H. Wu, Kay L. H. Yu, Hong-Ren Lee, Wei-Chia Hou, Chih-Yao Tain, You-Lin |
author_sort | Hsu, Chien-Ning |
collection | PubMed |
description | Gut microbiota-derived metabolites, in particular short chain fatty acids (SCFAs) and their receptors, are linked to hypertension. Fructose and antibiotics are commonly used worldwide, and they have a negative impact on the gut microbiota. Our previous study revealed that maternal high-fructose (HF) diet-induced hypertension in adult offspring is relevant to altered gut microbiome and its metabolites. We, therefore, intended to examine whether minocycline administration during pregnancy and lactation may further affect blood pressure (BP) programmed by maternal HF intake via mediating gut microbiota and SCFAs. Pregnant Sprague-Dawley rats received a normal diet or diet containing 60% fructose throughout pregnancy and lactation periods. Additionally, pregnant dams received minocycline (50 mg/kg/day) via oral gavage or a vehicle during pregnancy and lactation periods. Four groups of male offspring were studied (n = 8 per group): normal diet (ND), high-fructose diet (HF), normal diet + minocycline (NDM), and HF + minocycline (HFM). Male offspring were killed at 12 weeks of age. We observed that the HF diet and minocycline administration, both individually and together, causes the elevation of BP in adult male offspring, while there is no synergistic effect between them. Four groups displayed distinct enterotypes. Minocycline treatment leads to an increase in the F/B ratio, but decreased abundance of genera Lactobacillus, Ruminococcus, and Odoribacter. Additionally, minocycline treatment decreases plasma acetic acid and butyric acid levels. Hypertension programmed by maternal HF diet plus minocycline exposure is related to the increased expression of several SCFA receptors. Moreover, minocycline- and HF-induced hypertension, individually or together, is associated with the aberrant activation of the renin–angiotensin system (RAS). Conclusively, our results provide a new insight into the support of gut microbiota and its metabolite SCAFs in the developmental programming of hypertension and cast new light on the role of RAS in this process, which will help prevent hypertension programmed by maternal high-fructose and antibiotic exposure. |
format | Online Article Text |
id | pubmed-7961901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79619012021-03-17 Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure Hsu, Chien-Ning Chan, Julie Y. H. Wu, Kay L. H. Yu, Hong-Ren Lee, Wei-Chia Hou, Chih-Yao Tain, You-Lin Int J Mol Sci Article Gut microbiota-derived metabolites, in particular short chain fatty acids (SCFAs) and their receptors, are linked to hypertension. Fructose and antibiotics are commonly used worldwide, and they have a negative impact on the gut microbiota. Our previous study revealed that maternal high-fructose (HF) diet-induced hypertension in adult offspring is relevant to altered gut microbiome and its metabolites. We, therefore, intended to examine whether minocycline administration during pregnancy and lactation may further affect blood pressure (BP) programmed by maternal HF intake via mediating gut microbiota and SCFAs. Pregnant Sprague-Dawley rats received a normal diet or diet containing 60% fructose throughout pregnancy and lactation periods. Additionally, pregnant dams received minocycline (50 mg/kg/day) via oral gavage or a vehicle during pregnancy and lactation periods. Four groups of male offspring were studied (n = 8 per group): normal diet (ND), high-fructose diet (HF), normal diet + minocycline (NDM), and HF + minocycline (HFM). Male offspring were killed at 12 weeks of age. We observed that the HF diet and minocycline administration, both individually and together, causes the elevation of BP in adult male offspring, while there is no synergistic effect between them. Four groups displayed distinct enterotypes. Minocycline treatment leads to an increase in the F/B ratio, but decreased abundance of genera Lactobacillus, Ruminococcus, and Odoribacter. Additionally, minocycline treatment decreases plasma acetic acid and butyric acid levels. Hypertension programmed by maternal HF diet plus minocycline exposure is related to the increased expression of several SCFA receptors. Moreover, minocycline- and HF-induced hypertension, individually or together, is associated with the aberrant activation of the renin–angiotensin system (RAS). Conclusively, our results provide a new insight into the support of gut microbiota and its metabolite SCAFs in the developmental programming of hypertension and cast new light on the role of RAS in this process, which will help prevent hypertension programmed by maternal high-fructose and antibiotic exposure. MDPI 2021-03-06 /pmc/articles/PMC7961901/ /pubmed/33800916 http://dx.doi.org/10.3390/ijms22052674 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hsu, Chien-Ning Chan, Julie Y. H. Wu, Kay L. H. Yu, Hong-Ren Lee, Wei-Chia Hou, Chih-Yao Tain, You-Lin Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure |
title | Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure |
title_full | Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure |
title_fullStr | Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure |
title_full_unstemmed | Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure |
title_short | Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure |
title_sort | altered gut microbiota and its metabolites in hypertension of developmental origins: exploring differences between fructose and antibiotics exposure |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961901/ https://www.ncbi.nlm.nih.gov/pubmed/33800916 http://dx.doi.org/10.3390/ijms22052674 |
work_keys_str_mv | AT hsuchienning alteredgutmicrobiotaanditsmetabolitesinhypertensionofdevelopmentaloriginsexploringdifferencesbetweenfructoseandantibioticsexposure AT chanjulieyh alteredgutmicrobiotaanditsmetabolitesinhypertensionofdevelopmentaloriginsexploringdifferencesbetweenfructoseandantibioticsexposure AT wukaylh alteredgutmicrobiotaanditsmetabolitesinhypertensionofdevelopmentaloriginsexploringdifferencesbetweenfructoseandantibioticsexposure AT yuhongren alteredgutmicrobiotaanditsmetabolitesinhypertensionofdevelopmentaloriginsexploringdifferencesbetweenfructoseandantibioticsexposure AT leeweichia alteredgutmicrobiotaanditsmetabolitesinhypertensionofdevelopmentaloriginsexploringdifferencesbetweenfructoseandantibioticsexposure AT houchihyao alteredgutmicrobiotaanditsmetabolitesinhypertensionofdevelopmentaloriginsexploringdifferencesbetweenfructoseandantibioticsexposure AT tainyoulin alteredgutmicrobiotaanditsmetabolitesinhypertensionofdevelopmentaloriginsexploringdifferencesbetweenfructoseandantibioticsexposure |