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Detection of Urothelial Bladder Cancer Based on Urine and Tissue Telomerase Activity Measured by Novel RT-TRAP-2PCR Method

Purpose: To assess the diagnostic performance of urine telomerase activity (TA) in detecting bladder cancer (BCa) using the modified Telomeric Repeat Amplification Protocol (TRAP) and the Real Time Telomeric Repeat Amplification Protocol with double Polymerase Chain Reaction (RT-TRAP-2PCR). Methods:...

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Autores principales: Glukhov, Alexander, Potoldykova, Natalya, Taratkin, Mark, Gordeev, Sergey, Polyakovsky, Konstantin, Laukhtina, Ekaterina, Moschini, Marco, Abufaraj, Mohammad, Shariat, Shahrokh F., Sekacheva, Marina, Enikeev, Dmitry, Glybochko, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961950/
https://www.ncbi.nlm.nih.gov/pubmed/33806392
http://dx.doi.org/10.3390/jcm10051055
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author Glukhov, Alexander
Potoldykova, Natalya
Taratkin, Mark
Gordeev, Sergey
Polyakovsky, Konstantin
Laukhtina, Ekaterina
Moschini, Marco
Abufaraj, Mohammad
Shariat, Shahrokh F.
Sekacheva, Marina
Enikeev, Dmitry
Glybochko, Petr
author_facet Glukhov, Alexander
Potoldykova, Natalya
Taratkin, Mark
Gordeev, Sergey
Polyakovsky, Konstantin
Laukhtina, Ekaterina
Moschini, Marco
Abufaraj, Mohammad
Shariat, Shahrokh F.
Sekacheva, Marina
Enikeev, Dmitry
Glybochko, Petr
author_sort Glukhov, Alexander
collection PubMed
description Purpose: To assess the diagnostic performance of urine telomerase activity (TA) in detecting bladder cancer (BCa) using the modified Telomeric Repeat Amplification Protocol (TRAP) and the Real Time Telomeric Repeat Amplification Protocol with double Polymerase Chain Reaction (RT-TRAP-2PCR). Methods: In this case-control study, matching urine (in the pre- and post-surgical period) and tissue samples from 68 patients with BCa were assessed for TA. As a control, 45 urine samples were examined from non-BCa patients. TA levels were measured using TRAP and RT-TRAP-2 PCR methods. Results: Preoperative urinary TA was elevated in 64 (94.1%) of the 68 BCa patients. Urine TA was undetectable in 44 control patients, while TA was detected in one patient with histologically verified cystitis. Sensitivity for BCa detection of 94.1% and specificity of 97.8% were observed for urinary TA, while tissue TA had 100% sensitivity and 97.8% specificity. Both urine and tissue TA levels were not significantly higher in patients with muscle-invasive disease compared to those with non-muscle invasive BCa (p > 0.05). Urine and tissue TA levels were not associated with higher tumor grade, stage, and number of tumors (p > 0.05). However, the association was found between higher urinary and tissue TA levels with tumor size ≥ 3 cm (p = 0.02 and p = 0.01, respectively). During the first postoperative year, 17 BCa patients experienced disease recurrence, and urinary TA was present in 14 (82.4%) of these patients. The sensitivity and specificity of urinary TA levels for BCa recurrence in patients with non-muscle invasive bladder cancer (NMIBC) during follow-up were 82% and 94.4%, respectively. Conclusions: This pilot study demonstrates a high diagnostic performance of urinary and tissue TA levels measured by a new RT-TRAP-2PCR method for detecting and monitoring BCa. Additionally, the association was found between higher urinary and tissue TA levels with tumor size ≥ 3 cm; however, higher TA levels failed for significant correlation with advanced tumor stage and grade. Our study could serve as a benchmark for the evaluation of novel biomarkers using the RT-TRAP-2PCR method.
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spelling pubmed-79619502021-03-17 Detection of Urothelial Bladder Cancer Based on Urine and Tissue Telomerase Activity Measured by Novel RT-TRAP-2PCR Method Glukhov, Alexander Potoldykova, Natalya Taratkin, Mark Gordeev, Sergey Polyakovsky, Konstantin Laukhtina, Ekaterina Moschini, Marco Abufaraj, Mohammad Shariat, Shahrokh F. Sekacheva, Marina Enikeev, Dmitry Glybochko, Petr J Clin Med Article Purpose: To assess the diagnostic performance of urine telomerase activity (TA) in detecting bladder cancer (BCa) using the modified Telomeric Repeat Amplification Protocol (TRAP) and the Real Time Telomeric Repeat Amplification Protocol with double Polymerase Chain Reaction (RT-TRAP-2PCR). Methods: In this case-control study, matching urine (in the pre- and post-surgical period) and tissue samples from 68 patients with BCa were assessed for TA. As a control, 45 urine samples were examined from non-BCa patients. TA levels were measured using TRAP and RT-TRAP-2 PCR methods. Results: Preoperative urinary TA was elevated in 64 (94.1%) of the 68 BCa patients. Urine TA was undetectable in 44 control patients, while TA was detected in one patient with histologically verified cystitis. Sensitivity for BCa detection of 94.1% and specificity of 97.8% were observed for urinary TA, while tissue TA had 100% sensitivity and 97.8% specificity. Both urine and tissue TA levels were not significantly higher in patients with muscle-invasive disease compared to those with non-muscle invasive BCa (p > 0.05). Urine and tissue TA levels were not associated with higher tumor grade, stage, and number of tumors (p > 0.05). However, the association was found between higher urinary and tissue TA levels with tumor size ≥ 3 cm (p = 0.02 and p = 0.01, respectively). During the first postoperative year, 17 BCa patients experienced disease recurrence, and urinary TA was present in 14 (82.4%) of these patients. The sensitivity and specificity of urinary TA levels for BCa recurrence in patients with non-muscle invasive bladder cancer (NMIBC) during follow-up were 82% and 94.4%, respectively. Conclusions: This pilot study demonstrates a high diagnostic performance of urinary and tissue TA levels measured by a new RT-TRAP-2PCR method for detecting and monitoring BCa. Additionally, the association was found between higher urinary and tissue TA levels with tumor size ≥ 3 cm; however, higher TA levels failed for significant correlation with advanced tumor stage and grade. Our study could serve as a benchmark for the evaluation of novel biomarkers using the RT-TRAP-2PCR method. MDPI 2021-03-04 /pmc/articles/PMC7961950/ /pubmed/33806392 http://dx.doi.org/10.3390/jcm10051055 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Glukhov, Alexander
Potoldykova, Natalya
Taratkin, Mark
Gordeev, Sergey
Polyakovsky, Konstantin
Laukhtina, Ekaterina
Moschini, Marco
Abufaraj, Mohammad
Shariat, Shahrokh F.
Sekacheva, Marina
Enikeev, Dmitry
Glybochko, Petr
Detection of Urothelial Bladder Cancer Based on Urine and Tissue Telomerase Activity Measured by Novel RT-TRAP-2PCR Method
title Detection of Urothelial Bladder Cancer Based on Urine and Tissue Telomerase Activity Measured by Novel RT-TRAP-2PCR Method
title_full Detection of Urothelial Bladder Cancer Based on Urine and Tissue Telomerase Activity Measured by Novel RT-TRAP-2PCR Method
title_fullStr Detection of Urothelial Bladder Cancer Based on Urine and Tissue Telomerase Activity Measured by Novel RT-TRAP-2PCR Method
title_full_unstemmed Detection of Urothelial Bladder Cancer Based on Urine and Tissue Telomerase Activity Measured by Novel RT-TRAP-2PCR Method
title_short Detection of Urothelial Bladder Cancer Based on Urine and Tissue Telomerase Activity Measured by Novel RT-TRAP-2PCR Method
title_sort detection of urothelial bladder cancer based on urine and tissue telomerase activity measured by novel rt-trap-2pcr method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961950/
https://www.ncbi.nlm.nih.gov/pubmed/33806392
http://dx.doi.org/10.3390/jcm10051055
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