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Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice

Although physical exercise-induced autophagy activation has been considered a therapeutic target to enhance tissue health and extend lifespan, the effects of different exercise models on autophagy in specific metabolic tissues are not completely understood. This descriptive investigation compared th...

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Autores principales: Pinto, Ana P., da Rocha, Alisson L., Marafon, Bruno B., Rovina, Rafael L., Muñoz, Vitor R., da Silva, Lilian E. C. M., Pauli, José R., de Moura, Leandro P., Cintra, Dennys E., Ropelle, Eduardo R., da Silva, Adelino S. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962017/
https://www.ncbi.nlm.nih.gov/pubmed/33807902
http://dx.doi.org/10.3390/ijms22052635
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author Pinto, Ana P.
da Rocha, Alisson L.
Marafon, Bruno B.
Rovina, Rafael L.
Muñoz, Vitor R.
da Silva, Lilian E. C. M.
Pauli, José R.
de Moura, Leandro P.
Cintra, Dennys E.
Ropelle, Eduardo R.
da Silva, Adelino S. R.
author_facet Pinto, Ana P.
da Rocha, Alisson L.
Marafon, Bruno B.
Rovina, Rafael L.
Muñoz, Vitor R.
da Silva, Lilian E. C. M.
Pauli, José R.
de Moura, Leandro P.
Cintra, Dennys E.
Ropelle, Eduardo R.
da Silva, Adelino S. R.
author_sort Pinto, Ana P.
collection PubMed
description Although physical exercise-induced autophagy activation has been considered a therapeutic target to enhance tissue health and extend lifespan, the effects of different exercise models on autophagy in specific metabolic tissues are not completely understood. This descriptive investigation compared the acute effects of endurance (END), exhaustive (ET), strength (ST), and concurrent (CC) physical exercise protocols on markers of autophagy, genes, and proteins in the gastrocnemius muscle, heart, and liver of mice. The animals were euthanized immediately (0 h) and six hours (6 h) after the acute exercise for the measurement of glycogen levels, mRNA expression of Prkaa1, Ppargc1a, Mtor, Ulk1, Becn1, Atg5, Map1lc3b, Sqstm1, and protein levels of Beclin 1 and ATG5. The markers of autophagy were measured by quantifying the protein levels of LC3II and Sqstm1/p62 in response to three consecutive days of intraperitoneal injections of colchicine. In summary, for gastrocnemius muscle samples, the main alterations in mRNA expressions were observed after 6 h and for the ST group, and the markers of autophagy for the CC group were increased (i.e., LC3II and Sqstm1/p62). In the heart, the Beclin 1 and ATG5 levels were downregulated for the ET group. Regarding the markers of autophagy, the Sqstm1/p62 in the heart tissue was upregulated for the END and ST groups, highlighting the beneficial effects of these exercise models. The liver protein levels of ATG5 were downregulated for the ET group. After the colchicine treatment, the liver protein levels of Sqstm1/p62 were decreased for the END and ET groups compared to the CT, ST, and CC groups. These results could be related to diabetes and obesity development or liver dysfunction improvement, demanding further investigations.
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spelling pubmed-79620172021-03-17 Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice Pinto, Ana P. da Rocha, Alisson L. Marafon, Bruno B. Rovina, Rafael L. Muñoz, Vitor R. da Silva, Lilian E. C. M. Pauli, José R. de Moura, Leandro P. Cintra, Dennys E. Ropelle, Eduardo R. da Silva, Adelino S. R. Int J Mol Sci Article Although physical exercise-induced autophagy activation has been considered a therapeutic target to enhance tissue health and extend lifespan, the effects of different exercise models on autophagy in specific metabolic tissues are not completely understood. This descriptive investigation compared the acute effects of endurance (END), exhaustive (ET), strength (ST), and concurrent (CC) physical exercise protocols on markers of autophagy, genes, and proteins in the gastrocnemius muscle, heart, and liver of mice. The animals were euthanized immediately (0 h) and six hours (6 h) after the acute exercise for the measurement of glycogen levels, mRNA expression of Prkaa1, Ppargc1a, Mtor, Ulk1, Becn1, Atg5, Map1lc3b, Sqstm1, and protein levels of Beclin 1 and ATG5. The markers of autophagy were measured by quantifying the protein levels of LC3II and Sqstm1/p62 in response to three consecutive days of intraperitoneal injections of colchicine. In summary, for gastrocnemius muscle samples, the main alterations in mRNA expressions were observed after 6 h and for the ST group, and the markers of autophagy for the CC group were increased (i.e., LC3II and Sqstm1/p62). In the heart, the Beclin 1 and ATG5 levels were downregulated for the ET group. Regarding the markers of autophagy, the Sqstm1/p62 in the heart tissue was upregulated for the END and ST groups, highlighting the beneficial effects of these exercise models. The liver protein levels of ATG5 were downregulated for the ET group. After the colchicine treatment, the liver protein levels of Sqstm1/p62 were decreased for the END and ET groups compared to the CT, ST, and CC groups. These results could be related to diabetes and obesity development or liver dysfunction improvement, demanding further investigations. MDPI 2021-03-05 /pmc/articles/PMC7962017/ /pubmed/33807902 http://dx.doi.org/10.3390/ijms22052635 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pinto, Ana P.
da Rocha, Alisson L.
Marafon, Bruno B.
Rovina, Rafael L.
Muñoz, Vitor R.
da Silva, Lilian E. C. M.
Pauli, José R.
de Moura, Leandro P.
Cintra, Dennys E.
Ropelle, Eduardo R.
da Silva, Adelino S. R.
Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice
title Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice
title_full Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice
title_fullStr Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice
title_full_unstemmed Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice
title_short Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice
title_sort impact of different physical exercises on the expression of autophagy markers in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962017/
https://www.ncbi.nlm.nih.gov/pubmed/33807902
http://dx.doi.org/10.3390/ijms22052635
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