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Differential effects of intense exercise and pollution on the airways in a murine model
BACKGROUND: Exercise-induced bronchoconstriction (EIB) is a transient airway narrowing, occurring during or shortly after intensive exercise. It is highly prevalent in non-asthmatic outdoor endurance athletes suggesting an important contribution of air pollution in the development of EIB. Therefore,...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962283/ https://www.ncbi.nlm.nih.gov/pubmed/33722268 http://dx.doi.org/10.1186/s12989-021-00401-6 |
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author | Decaesteker, Tatjana Vanhoffelen, Eliane Trekels, Kristel Jonckheere, Anne-Charlotte Cremer, Jonathan Vanstapel, Arno Dilissen, Ellen Bullens, Dominique Dupont, Lieven J. Vanoirbeek, Jeroen A. |
author_facet | Decaesteker, Tatjana Vanhoffelen, Eliane Trekels, Kristel Jonckheere, Anne-Charlotte Cremer, Jonathan Vanstapel, Arno Dilissen, Ellen Bullens, Dominique Dupont, Lieven J. Vanoirbeek, Jeroen A. |
author_sort | Decaesteker, Tatjana |
collection | PubMed |
description | BACKGROUND: Exercise-induced bronchoconstriction (EIB) is a transient airway narrowing, occurring during or shortly after intensive exercise. It is highly prevalent in non-asthmatic outdoor endurance athletes suggesting an important contribution of air pollution in the development of EIB. Therefore, more research is necessary to investigate the combination of exercise and pollutants on the airways. METHODS: Balbc/ByJ mice were intranasally challenged 5 days a week for 3 weeks with saline or 0.2 mg/ml diesel exhaust particles (DEP), prior to a daily incremental running session or non-exercise session. Once a week, the early ventilatory response was measured and lung function was determined at day 24. Airway inflammation and cytokine levels were evaluated in bronchoalveolar lavage fluid. Furthermore, innate lymphoid cells, dendritic cells and tight junction mRNA expression were determined in lung tissue. RESULTS: Submaximal exercise resulted in acute alterations of the breathing pattern and significantly improved FEV(0.1) at day 24. DEP exposure induced neutrophilic airway inflammation, accompanied with increased percentages of CD11b(+) DC in lung tissue and pro-inflammatory cytokines, such as IL-13, MCP-1, GM-CSF and KC. Occludin and claudin-1(Cldn-1) expression were respectively increased and decreased by DEP exposure. Whereas, exercise increased Cldn-3 and Cldn-18 expression. Combining exercise and DEP exposure resulted in significantly increased SP-D levels in the airways. CONCLUSION: DEP exposure induced typical airway neutrophilia, DC recruitment and pro-inflammatory cytokine production. Whereas, intensive exercise induced changes of the breathing pattern. The combination of both triggers resulted in a dysregulation of tight junction expression, suggesting that intensive exercise in polluted environments can induce important changes in the airway physiology and integrity. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12989-021-00401-6. |
format | Online Article Text |
id | pubmed-7962283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-79622832021-03-16 Differential effects of intense exercise and pollution on the airways in a murine model Decaesteker, Tatjana Vanhoffelen, Eliane Trekels, Kristel Jonckheere, Anne-Charlotte Cremer, Jonathan Vanstapel, Arno Dilissen, Ellen Bullens, Dominique Dupont, Lieven J. Vanoirbeek, Jeroen A. Part Fibre Toxicol Research BACKGROUND: Exercise-induced bronchoconstriction (EIB) is a transient airway narrowing, occurring during or shortly after intensive exercise. It is highly prevalent in non-asthmatic outdoor endurance athletes suggesting an important contribution of air pollution in the development of EIB. Therefore, more research is necessary to investigate the combination of exercise and pollutants on the airways. METHODS: Balbc/ByJ mice were intranasally challenged 5 days a week for 3 weeks with saline or 0.2 mg/ml diesel exhaust particles (DEP), prior to a daily incremental running session or non-exercise session. Once a week, the early ventilatory response was measured and lung function was determined at day 24. Airway inflammation and cytokine levels were evaluated in bronchoalveolar lavage fluid. Furthermore, innate lymphoid cells, dendritic cells and tight junction mRNA expression were determined in lung tissue. RESULTS: Submaximal exercise resulted in acute alterations of the breathing pattern and significantly improved FEV(0.1) at day 24. DEP exposure induced neutrophilic airway inflammation, accompanied with increased percentages of CD11b(+) DC in lung tissue and pro-inflammatory cytokines, such as IL-13, MCP-1, GM-CSF and KC. Occludin and claudin-1(Cldn-1) expression were respectively increased and decreased by DEP exposure. Whereas, exercise increased Cldn-3 and Cldn-18 expression. Combining exercise and DEP exposure resulted in significantly increased SP-D levels in the airways. CONCLUSION: DEP exposure induced typical airway neutrophilia, DC recruitment and pro-inflammatory cytokine production. Whereas, intensive exercise induced changes of the breathing pattern. The combination of both triggers resulted in a dysregulation of tight junction expression, suggesting that intensive exercise in polluted environments can induce important changes in the airway physiology and integrity. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12989-021-00401-6. BioMed Central 2021-03-15 /pmc/articles/PMC7962283/ /pubmed/33722268 http://dx.doi.org/10.1186/s12989-021-00401-6 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Decaesteker, Tatjana Vanhoffelen, Eliane Trekels, Kristel Jonckheere, Anne-Charlotte Cremer, Jonathan Vanstapel, Arno Dilissen, Ellen Bullens, Dominique Dupont, Lieven J. Vanoirbeek, Jeroen A. Differential effects of intense exercise and pollution on the airways in a murine model |
title | Differential effects of intense exercise and pollution on the airways in a murine model |
title_full | Differential effects of intense exercise and pollution on the airways in a murine model |
title_fullStr | Differential effects of intense exercise and pollution on the airways in a murine model |
title_full_unstemmed | Differential effects of intense exercise and pollution on the airways in a murine model |
title_short | Differential effects of intense exercise and pollution on the airways in a murine model |
title_sort | differential effects of intense exercise and pollution on the airways in a murine model |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962283/ https://www.ncbi.nlm.nih.gov/pubmed/33722268 http://dx.doi.org/10.1186/s12989-021-00401-6 |
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