Cargando…

Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19

Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow cytometry-based serological and neutralizati...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tian, Linhua, Elsheikh, Elzafir B., Patrone, Paul N., Kearsley, Anthony J., Gaigalas, Adolfas K., Inwood, Sarah, Lin-Gibson, Sheng, Esposito, Dominic, Wang, Lili
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962843/ https://www.ncbi.nlm.nih.gov/pubmed/33800363 http://dx.doi.org/10.3390/ijms22052723

Ejemplares similares

Development of a Cell-Based SARS-CoV-2 Pseudovirus Neutralization Assay Using Imaging and Flow Cytometry Analysis
por: Izac, Jerilyn R., et al.
Publicado: (2023)

Monoclonal Antibodies as SARS-CoV-2 Serology Standards: Experimental Validation and Broader Implications for Correlates of Protection
por: Wang, Lili, et al.
Publicado: (2023)

Development of Multicolor Flow Cytometry Calibration Standards: Assignment of Equivalent Reference Fluorophores (ERF) Unit
por: Wang, Lili, et al.
Publicado: (2011)

Stochastic Reaction–Diffusion Model of the Binding of Monoclonal Antibodies to CD4 Receptors on the Surface of T Cells
por: Wang, Lili, et al.
Publicado: (2020)

Sources of Variability in the Response of Labeled Microspheres and B Cells during the Analysis by a Flow Cytometer
por: Gaigalas, Adolfas K., et al.
Publicado: (2021)

Number Concentration Measurements of Polystyrene Submicrometer Particles
por: DeRose, Paul C., et al.
Publicado: (2022)

Measurement of the Fluorescence Quantum Yield Using a Spectrometer With an Integrating Sphere Detector
por: Gaigalas, Adolfas K., et al.
Publicado: (2008)

Modeling of Photochemical Reactions in a Focused Laser Beam, II
por: Gaigalas, Adolfas K., et al.
Publicado: (2008)

Quantitating Fluorescence Intensity From Fluorophores: Practical Use of MESF Values
por: Wang, Lili, et al.
Publicado: (2002)

Expanding NIST Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles
por: DeRose, Paul, et al.
Publicado: (2020)

Quantitating Fluorescence Intensity from Fluorophore: The Definition of MESF Assignment
por: Schwartz, Abe, et al.
Publicado: (2002)

Optical Properties of CdSe/ZnS Nanocrystals
por: Gaigalas, Adolfas K, et al.
Publicado: (2014)

Measurement of Scattering and Absorption Cross Sections of Dyed Microspheres
por: Gaigalas, Adolfas K, et al.
Publicado: (2013)

Affine analysis for quantitative PCR measurements
por: Patrone, Paul N., et al.
Publicado: (2020)

The WHO International Standard for COVID-19 serological tests: towards harmonization of anti-spike assays
por: Infantino, Maria, et al.
Publicado: (2021)

The HPV Serology Laboratory leads an initiative to standardize and harmonize human papillomavirus serology assays
por: Park, Isabel, et al.
Publicado: (2023)

A semi-quantitative, rapid, point of care SARS-CoV-2 serologic assay predicts neutralizing antibody levels
por: Markmann, Alena J., et al.
Publicado: (2023)

Correlation between In Vitro Neutralization Assay and Serological Tests for Protective Antibodies Detection
por: Bonifacio, Maria Addolorata, et al.
Publicado: (2022)

Optimization of a Quantitative Micro-neutralization Assay
por: Lin, Yipu, et al.
Publicado: (2016)

Evaluation of a Multiparametric Immunofluorescence Assay for Standardization of Neuromyelitis Optica Serology
por: Granieri, Letizia, et al.
Publicado: (2012)

An Assessment of Serological Assays for SARS-CoV-2 as Surrogates for Authentic Virus Neutralization
por: Wohlgemuth, Nicholas, et al.
Publicado: (2021)

Evaluation of a Quantitative Serological Assay for Diagnosing Chronic Pulmonary Aspergillosis
por: Fujiuchi, Satoru, et al.
Publicado: (2016)

Optimal Decision Theory for Diagnostic Testing: Minimizing Indeterminate Classes with Applications to Saliva-Based SARS-CoV-2 Antibody Assays
por: Patrone, Paul N., et al.
Publicado: (2022)

Optimal decision theory for diagnostic testing: Minimizing indeterminate classes with applications to saliva-based SARS-CoV-2 antibody assays
por: Patrone, Paul N., et al.
Publicado: (2022)

Establishing CD19 B-cell reference control materials for comparable and quantitative cytometric expression analysis
por: Wang, Lili, et al.
Publicado: (2021)

SARS-CoV-2-Neutralizing Antibody Response and Correlation of Two Serological Assays with Microneutralization
por: Souiri, Amal, et al.
Publicado: (2023)

Modeling in higher dimensions to improve diagnostic testing accuracy: theory and examples for multiplex saliva-based SARS-CoV-2 antibody assays
por: Luke, Rayanne A., et al.
Publicado: (2022)

Modeling in higher dimensions to improve diagnostic testing accuracy: Theory and examples for multiplex saliva-based SARS-CoV-2 antibody assays
por: Luke, Rayanne A., et al.
Publicado: (2023)

Overview of Neutralization Assays and International Standard for Detecting SARS-CoV-2 Neutralizing Antibody
por: Liu, Kuan-Ting, et al.
Publicado: (2022)

Standardization and Evaluation of an Anti-ZIKV IgM ELISA Assay for the Serological Diagnosis of Zika Virus Infection
por: Sirikajornpan, Kanittha, et al.
Publicado: (2021)

Quantitating Fluorescence Intensity From Fluorophore: Assignment of MESF Values
por: Gaigalas, A. K., et al.
Publicado: (2005)

Serological reactivity of inactivated SARS-CoV-2 vaccine based on an S-RBD neutralizing antibody assay
por: Zhang, Hui, et al.
Publicado: (2022)

A serological aptamer-assisted proximity ligation assay for COVID-19 diagnosis and seeking neutralizing aptamers
por: Liu, Ran, et al.
Publicado: (2020)

Optofluidic flow meter for sub-nanoliter per minute flow measurements
por: Sadeghi, Jalal, et al.
Publicado: (2022)

Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material
por: Freeman, James, et al.
Publicado: (2022)

Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays
por: Mehalko, Jennifer, et al.
Publicado: (2020)

Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays
por: Mehalko, Jennifer, et al.
Publicado: (2021)

SARS-CoV-2 Serologic Assays in Control and Unknown Populations Demonstrate the Necessity of Virus Neutralization Testing
por: Rathe, Jennifer A, et al.
Publicado: (2020)

Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays
por: Esposito, Dominic, et al.
Publicado: (2020)

Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays
por: Esposito, Dominic, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_qf4og9mli6aprin1s3cekvckcq