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Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

Objectives  While DNA profiling has become the principal technique for individualization of biological evidences, ABO blood grouping is still a useful test method in the initial stages of crime investigation. Objectives of the study were blood group determination using slide agglutination method, bl...

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Autores principales: Tejasvi, M.L Avinash, Bukkya, Jaya Laksmi, Rao, Pandu Ranga, Bhayya, Harsha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Georg Thieme Verlag KG 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7964258/
https://www.ncbi.nlm.nih.gov/pubmed/33748820
http://dx.doi.org/10.1055/s-0041-1723083
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author Tejasvi, M.L Avinash
Bukkya, Jaya Laksmi
Rao, Pandu Ranga
Bhayya, Harsha
author_facet Tejasvi, M.L Avinash
Bukkya, Jaya Laksmi
Rao, Pandu Ranga
Bhayya, Harsha
author_sort Tejasvi, M.L Avinash
collection PubMed
description Objectives  While DNA profiling has become the principal technique for individualization of biological evidences, ABO blood grouping is still a useful test method in the initial stages of crime investigation. Objectives of the study were blood group determination using slide agglutination method, blood group determination from saliva using absorption inhibition method, and comparison of slide agglutination method with that of absorption inhibition method from saliva sample. Materials and Methods  A total of 60 subjects were taken randomly with their age ranging from 20 to 60 years. Sixty subjects were divided in to two groups, study group and control group. 5 to 10 mL of unstimulated saliva was collected from 60 patients and Wieners agglutination test was performed to detect the secretor status of blood using absorption inhibition method and compared with that of slide agglutination method Results  Out of 60 subjects, 52 subjects showed secretors of antigen in saliva with percentage value of 86.66% and eight subjects were nonsecretors (13.33%). Slightly higher percentage of secretor status was seen in males 84.6 and 88.2% in females. Conclusion  Evaluation of secretor status of blood group antigen from saliva using absorption inhibition method can be useful method in identification of medicolegal cases.
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spelling pubmed-79642582021-03-18 Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method Tejasvi, M.L Avinash Bukkya, Jaya Laksmi Rao, Pandu Ranga Bhayya, Harsha Glob Med Genet Objectives  While DNA profiling has become the principal technique for individualization of biological evidences, ABO blood grouping is still a useful test method in the initial stages of crime investigation. Objectives of the study were blood group determination using slide agglutination method, blood group determination from saliva using absorption inhibition method, and comparison of slide agglutination method with that of absorption inhibition method from saliva sample. Materials and Methods  A total of 60 subjects were taken randomly with their age ranging from 20 to 60 years. Sixty subjects were divided in to two groups, study group and control group. 5 to 10 mL of unstimulated saliva was collected from 60 patients and Wieners agglutination test was performed to detect the secretor status of blood using absorption inhibition method and compared with that of slide agglutination method Results  Out of 60 subjects, 52 subjects showed secretors of antigen in saliva with percentage value of 86.66% and eight subjects were nonsecretors (13.33%). Slightly higher percentage of secretor status was seen in males 84.6 and 88.2% in females. Conclusion  Evaluation of secretor status of blood group antigen from saliva using absorption inhibition method can be useful method in identification of medicolegal cases. Georg Thieme Verlag KG 2021-03 2021-02-23 /pmc/articles/PMC7964258/ /pubmed/33748820 http://dx.doi.org/10.1055/s-0041-1723083 Text en The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. ( https://creativecommons.org/licenses/by/4.0/ ) https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Tejasvi, M.L Avinash
Bukkya, Jaya Laksmi
Rao, Pandu Ranga
Bhayya, Harsha
Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method
title Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method
title_full Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method
title_fullStr Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method
title_full_unstemmed Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method
title_short Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method
title_sort evaluation of the secretor status of abo blood group antigens in saliva using absorption inhibition method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7964258/
https://www.ncbi.nlm.nih.gov/pubmed/33748820
http://dx.doi.org/10.1055/s-0041-1723083
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