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Natural Transformation of Riemerella columbina and Its Determinants
In a previous study, it was shown that Riemerella anatipestifer, a member of Flavobacteriaceae, is naturally competent. However, whether natural competence is universal in Flavobacteriaceae remains unknown. In this study, it was shown for the first time that Riemerella columbina was naturally compet...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7965970/ https://www.ncbi.nlm.nih.gov/pubmed/33746928 http://dx.doi.org/10.3389/fmicb.2021.634895 |
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author | Huang, Li Liu, Mafeng Zhu, Dekang Xie, Li Huang, Mi Xiang, Chen Biville, Francis Jia, Renyong Chen, Shun Zhao, Xinxin Yang, Qiao Wu, Ying Zhang, Shaqiu Huang, Juan Ou, Xumin Mao, Sai Gao, Qun Sun, Di Tian, Bin Wang, Mingshu Cheng, Anchun |
author_facet | Huang, Li Liu, Mafeng Zhu, Dekang Xie, Li Huang, Mi Xiang, Chen Biville, Francis Jia, Renyong Chen, Shun Zhao, Xinxin Yang, Qiao Wu, Ying Zhang, Shaqiu Huang, Juan Ou, Xumin Mao, Sai Gao, Qun Sun, Di Tian, Bin Wang, Mingshu Cheng, Anchun |
author_sort | Huang, Li |
collection | PubMed |
description | In a previous study, it was shown that Riemerella anatipestifer, a member of Flavobacteriaceae, is naturally competent. However, whether natural competence is universal in Flavobacteriaceae remains unknown. In this study, it was shown for the first time that Riemerella columbina was naturally competent in the laboratory condition; however, Flavobacterium johnsoniae was not naturally competent under the same conditions. The competence of R. columbina was maintained throughout the growth phases, and the transformation frequency was highest during the logarithmic phase. A competition assay revealed that R. columbina preferentially took up its own genomic DNA over heterologous DNA. The natural transformation frequency of R. columbina was significantly increased in GCB medium without peptone or phosphate. Furthermore, natural transformation of R. columbina was inhibited by 0.5 mM EDTA, but could be restored by the addition of CaCl(2), MgCl(2), ZnCl(2), and MnCl(2), suggesting that these divalent cations promote the natural transformation of R. columbina. Overall, this study revealed that natural competence is not universal in Flavobacteriaceae members and triggering of competence differs from species to species. |
format | Online Article Text |
id | pubmed-7965970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79659702021-03-18 Natural Transformation of Riemerella columbina and Its Determinants Huang, Li Liu, Mafeng Zhu, Dekang Xie, Li Huang, Mi Xiang, Chen Biville, Francis Jia, Renyong Chen, Shun Zhao, Xinxin Yang, Qiao Wu, Ying Zhang, Shaqiu Huang, Juan Ou, Xumin Mao, Sai Gao, Qun Sun, Di Tian, Bin Wang, Mingshu Cheng, Anchun Front Microbiol Microbiology In a previous study, it was shown that Riemerella anatipestifer, a member of Flavobacteriaceae, is naturally competent. However, whether natural competence is universal in Flavobacteriaceae remains unknown. In this study, it was shown for the first time that Riemerella columbina was naturally competent in the laboratory condition; however, Flavobacterium johnsoniae was not naturally competent under the same conditions. The competence of R. columbina was maintained throughout the growth phases, and the transformation frequency was highest during the logarithmic phase. A competition assay revealed that R. columbina preferentially took up its own genomic DNA over heterologous DNA. The natural transformation frequency of R. columbina was significantly increased in GCB medium without peptone or phosphate. Furthermore, natural transformation of R. columbina was inhibited by 0.5 mM EDTA, but could be restored by the addition of CaCl(2), MgCl(2), ZnCl(2), and MnCl(2), suggesting that these divalent cations promote the natural transformation of R. columbina. Overall, this study revealed that natural competence is not universal in Flavobacteriaceae members and triggering of competence differs from species to species. Frontiers Media S.A. 2021-03-03 /pmc/articles/PMC7965970/ /pubmed/33746928 http://dx.doi.org/10.3389/fmicb.2021.634895 Text en Copyright © 2021 Huang, Liu, Zhu, Xie, Huang, Xiang, Biville, Jia, Chen, Zhao, Yang, Wu, Zhang, Huang, Ou, Mao, Gao, Sun, Tian, Wang and Cheng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Huang, Li Liu, Mafeng Zhu, Dekang Xie, Li Huang, Mi Xiang, Chen Biville, Francis Jia, Renyong Chen, Shun Zhao, Xinxin Yang, Qiao Wu, Ying Zhang, Shaqiu Huang, Juan Ou, Xumin Mao, Sai Gao, Qun Sun, Di Tian, Bin Wang, Mingshu Cheng, Anchun Natural Transformation of Riemerella columbina and Its Determinants |
title | Natural Transformation of Riemerella columbina and Its Determinants |
title_full | Natural Transformation of Riemerella columbina and Its Determinants |
title_fullStr | Natural Transformation of Riemerella columbina and Its Determinants |
title_full_unstemmed | Natural Transformation of Riemerella columbina and Its Determinants |
title_short | Natural Transformation of Riemerella columbina and Its Determinants |
title_sort | natural transformation of riemerella columbina and its determinants |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7965970/ https://www.ncbi.nlm.nih.gov/pubmed/33746928 http://dx.doi.org/10.3389/fmicb.2021.634895 |
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