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Macrophage polarization in peri-implantitis lesions

OBJECTIVES: To immunohistochemically characterize and correlate macrophage M1/M2 polarization status with disease severity at peri-implantitis sites. MATERIALS AND METHODS: A total of twenty patients (n = 20 implants) diagnosed with peri-implantitis (i.e., bleeding on probing with or without suppura...

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Autores principales: Galarraga-Vinueza, Maria Elisa, Obreja, Karina, Ramanauskaite, Ausra, Magini, Ricardo, Begic, Amira, Sader, Robert, Schwarz, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966129/
https://www.ncbi.nlm.nih.gov/pubmed/32886246
http://dx.doi.org/10.1007/s00784-020-03556-2
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author Galarraga-Vinueza, Maria Elisa
Obreja, Karina
Ramanauskaite, Ausra
Magini, Ricardo
Begic, Amira
Sader, Robert
Schwarz, Frank
author_facet Galarraga-Vinueza, Maria Elisa
Obreja, Karina
Ramanauskaite, Ausra
Magini, Ricardo
Begic, Amira
Sader, Robert
Schwarz, Frank
author_sort Galarraga-Vinueza, Maria Elisa
collection PubMed
description OBJECTIVES: To immunohistochemically characterize and correlate macrophage M1/M2 polarization status with disease severity at peri-implantitis sites. MATERIALS AND METHODS: A total of twenty patients (n = 20 implants) diagnosed with peri-implantitis (i.e., bleeding on probing with or without suppuration, probing depths ≥ 6 mm, and radiographic marginal bone loss ≥ 3 mm) were included. The severity of peri-implantitis was classified according to established criteria (i.e., slight, moderate, and advanced). Granulation tissue biopsies were obtained during surgical therapy and prepared for immunohistological assessment and macrophage polarization characterization. Macrophages, M1, and M2 phenotypes were identified through immunohistochemical markers (i.e., CD68, CD80, and CD206) and quantified through histomorphometrical analyses. RESULTS: Macrophages exhibiting a positive CD68 expression occupied a mean proportion of 14.36% (95% CI 11.4–17.2) of the inflammatory connective tissue (ICT) area. Positive M1 (CD80) and M2 (CD206) macrophages occupied a mean value of 7.07% (95% CI 5.9–9.4) and 5.22% (95% CI 3.8–6.6) of the ICT, respectively. The mean M1/M2 ratio was 1.56 (95% CI 1–12–1.9). Advanced peri-implantitis cases expressed a significantly higher M1 (%) when compared with M2 (%) expression. There was a significant correlation between CD68 (%) and M1 (%) expression and probing depth (PD) values. CONCLUSION: The present immunohistochemical analysis suggests that macrophages constitute a considerable proportion of the inflammatory cellular composition at peri-implantitis sites, revealing a significant higher expression for M1 inflammatory phenotype at advanced peri-implantitis sites, which could possibly play a critical role in disease progression. CLINICAL RELEVANCE: Macrophages have critical functions to establish homeostasis and disease. Bacteria might induce oral dysbiosis unbalancing the host’s immunological response and triggering inflammation around dental implants. M1/M2 status could possibly reveal peri-implantitis’ underlying pathogenesis.
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spelling pubmed-79661292021-04-01 Macrophage polarization in peri-implantitis lesions Galarraga-Vinueza, Maria Elisa Obreja, Karina Ramanauskaite, Ausra Magini, Ricardo Begic, Amira Sader, Robert Schwarz, Frank Clin Oral Investig Original Article OBJECTIVES: To immunohistochemically characterize and correlate macrophage M1/M2 polarization status with disease severity at peri-implantitis sites. MATERIALS AND METHODS: A total of twenty patients (n = 20 implants) diagnosed with peri-implantitis (i.e., bleeding on probing with or without suppuration, probing depths ≥ 6 mm, and radiographic marginal bone loss ≥ 3 mm) were included. The severity of peri-implantitis was classified according to established criteria (i.e., slight, moderate, and advanced). Granulation tissue biopsies were obtained during surgical therapy and prepared for immunohistological assessment and macrophage polarization characterization. Macrophages, M1, and M2 phenotypes were identified through immunohistochemical markers (i.e., CD68, CD80, and CD206) and quantified through histomorphometrical analyses. RESULTS: Macrophages exhibiting a positive CD68 expression occupied a mean proportion of 14.36% (95% CI 11.4–17.2) of the inflammatory connective tissue (ICT) area. Positive M1 (CD80) and M2 (CD206) macrophages occupied a mean value of 7.07% (95% CI 5.9–9.4) and 5.22% (95% CI 3.8–6.6) of the ICT, respectively. The mean M1/M2 ratio was 1.56 (95% CI 1–12–1.9). Advanced peri-implantitis cases expressed a significantly higher M1 (%) when compared with M2 (%) expression. There was a significant correlation between CD68 (%) and M1 (%) expression and probing depth (PD) values. CONCLUSION: The present immunohistochemical analysis suggests that macrophages constitute a considerable proportion of the inflammatory cellular composition at peri-implantitis sites, revealing a significant higher expression for M1 inflammatory phenotype at advanced peri-implantitis sites, which could possibly play a critical role in disease progression. CLINICAL RELEVANCE: Macrophages have critical functions to establish homeostasis and disease. Bacteria might induce oral dysbiosis unbalancing the host’s immunological response and triggering inflammation around dental implants. M1/M2 status could possibly reveal peri-implantitis’ underlying pathogenesis. Springer Berlin Heidelberg 2020-09-04 2021 /pmc/articles/PMC7966129/ /pubmed/32886246 http://dx.doi.org/10.1007/s00784-020-03556-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Galarraga-Vinueza, Maria Elisa
Obreja, Karina
Ramanauskaite, Ausra
Magini, Ricardo
Begic, Amira
Sader, Robert
Schwarz, Frank
Macrophage polarization in peri-implantitis lesions
title Macrophage polarization in peri-implantitis lesions
title_full Macrophage polarization in peri-implantitis lesions
title_fullStr Macrophage polarization in peri-implantitis lesions
title_full_unstemmed Macrophage polarization in peri-implantitis lesions
title_short Macrophage polarization in peri-implantitis lesions
title_sort macrophage polarization in peri-implantitis lesions
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966129/
https://www.ncbi.nlm.nih.gov/pubmed/32886246
http://dx.doi.org/10.1007/s00784-020-03556-2
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