Cargando…
Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema
OBJECTIVE: It is well known that regular turmeric extract with 95% curcuminoid is comprised of curcumin (70.07%), desmethoxycurcumin (20.28%), and bisdemethoxycurcumin (BDMC) (3.63%). In the current study for the first time, we have enriched about 3% of bisdemethoxycurcumin (BDMC) to 70% as well as...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966389/ https://www.ncbi.nlm.nih.gov/pubmed/33737826 http://dx.doi.org/10.2147/JIR.S291293 |
_version_ | 1783665691993309184 |
---|---|
author | Gouthamchandra, Kuluvar Sudeep, Heggar Venkataramana Chandrappa, Siddappa Raj, Amrith Naveen, Puttaswamy Shyamaprasad, Kodimule |
author_facet | Gouthamchandra, Kuluvar Sudeep, Heggar Venkataramana Chandrappa, Siddappa Raj, Amrith Naveen, Puttaswamy Shyamaprasad, Kodimule |
author_sort | Gouthamchandra, Kuluvar |
collection | PubMed |
description | OBJECTIVE: It is well known that regular turmeric extract with 95% curcuminoid is comprised of curcumin (70.07%), desmethoxycurcumin (20.28%), and bisdemethoxycurcumin (BDMC) (3.63%). In the current study for the first time, we have enriched about 3% of bisdemethoxycurcumin (BDMC) to 70% as well as named it as REVERC3 and compared anti-inflammatory activity with regular turmeric extract using in vitro and in vivo models of inflammation. METHODS: To reveal the potential anti-inflammatory mechanism of action, we investigated nitric oxide (NO) scavenging, xanthine oxidase, and lipoxygenase inhibitory activity, further determined the level of pro-inflammatory cytokines, such as interleukin 6 (IL-6), tumor necrosis factor (TNF-α) and major inflammatory mediators like cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS), inhibition in lipopolysaccharide (LPS) induced inflammation in RAW macrophage cells. In the other hand, a carrageenan-stimulated inflammatory rat model was carried out. RESULTS: Our study findings exhibited a significant anti-inflammatory activity of REVERC3 together with nitric oxide (NO), xanthine oxidase, and lipoxygenase inhibition. Further, we attenuated the levels of cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS), interleukin (IL-6) and tumor necrosis factor (TNF-α) expressions in the LPS-elicited RAW macrophage cells. REVERC3 showed a potential anti-inflammatory activity by inhibiting carrageenan induced paw edema after 4 hr at the dose of 100mg/kg body weight. CONCLUSION: Thus, our findings collectively indicated that the REVERC3 could efficiently inhibit inflammation compared to regular turmeric extract. Since bisdemethoxycurcumin is a stable molecule it could be effectively used in the applications of health care and the nutraceutical industry, indeed which deserves further investigations. |
format | Online Article Text |
id | pubmed-7966389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-79663892021-03-17 Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema Gouthamchandra, Kuluvar Sudeep, Heggar Venkataramana Chandrappa, Siddappa Raj, Amrith Naveen, Puttaswamy Shyamaprasad, Kodimule J Inflamm Res Original Research OBJECTIVE: It is well known that regular turmeric extract with 95% curcuminoid is comprised of curcumin (70.07%), desmethoxycurcumin (20.28%), and bisdemethoxycurcumin (BDMC) (3.63%). In the current study for the first time, we have enriched about 3% of bisdemethoxycurcumin (BDMC) to 70% as well as named it as REVERC3 and compared anti-inflammatory activity with regular turmeric extract using in vitro and in vivo models of inflammation. METHODS: To reveal the potential anti-inflammatory mechanism of action, we investigated nitric oxide (NO) scavenging, xanthine oxidase, and lipoxygenase inhibitory activity, further determined the level of pro-inflammatory cytokines, such as interleukin 6 (IL-6), tumor necrosis factor (TNF-α) and major inflammatory mediators like cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS), inhibition in lipopolysaccharide (LPS) induced inflammation in RAW macrophage cells. In the other hand, a carrageenan-stimulated inflammatory rat model was carried out. RESULTS: Our study findings exhibited a significant anti-inflammatory activity of REVERC3 together with nitric oxide (NO), xanthine oxidase, and lipoxygenase inhibition. Further, we attenuated the levels of cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS), interleukin (IL-6) and tumor necrosis factor (TNF-α) expressions in the LPS-elicited RAW macrophage cells. REVERC3 showed a potential anti-inflammatory activity by inhibiting carrageenan induced paw edema after 4 hr at the dose of 100mg/kg body weight. CONCLUSION: Thus, our findings collectively indicated that the REVERC3 could efficiently inhibit inflammation compared to regular turmeric extract. Since bisdemethoxycurcumin is a stable molecule it could be effectively used in the applications of health care and the nutraceutical industry, indeed which deserves further investigations. Dove 2021-03-12 /pmc/articles/PMC7966389/ /pubmed/33737826 http://dx.doi.org/10.2147/JIR.S291293 Text en © 2021 Gouthamchandra et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Gouthamchandra, Kuluvar Sudeep, Heggar Venkataramana Chandrappa, Siddappa Raj, Amrith Naveen, Puttaswamy Shyamaprasad, Kodimule Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema |
title | Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema |
title_full | Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema |
title_fullStr | Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema |
title_full_unstemmed | Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema |
title_short | Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema |
title_sort | efficacy of a standardized turmeric extract comprised of 70% bisdemothoxy-curcumin (reverc3) against lps-induced inflammation in raw264.7 cells and carrageenan-induced paw edema |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966389/ https://www.ncbi.nlm.nih.gov/pubmed/33737826 http://dx.doi.org/10.2147/JIR.S291293 |
work_keys_str_mv | AT gouthamchandrakuluvar efficacyofastandardizedturmericextractcomprisedof70bisdemothoxycurcuminreverc3againstlpsinducedinflammationinraw2647cellsandcarrageenaninducedpawedema AT sudeepheggarvenkataramana efficacyofastandardizedturmericextractcomprisedof70bisdemothoxycurcuminreverc3againstlpsinducedinflammationinraw2647cellsandcarrageenaninducedpawedema AT chandrappasiddappa efficacyofastandardizedturmericextractcomprisedof70bisdemothoxycurcuminreverc3againstlpsinducedinflammationinraw2647cellsandcarrageenaninducedpawedema AT rajamrith efficacyofastandardizedturmericextractcomprisedof70bisdemothoxycurcuminreverc3againstlpsinducedinflammationinraw2647cellsandcarrageenaninducedpawedema AT naveenputtaswamy efficacyofastandardizedturmericextractcomprisedof70bisdemothoxycurcuminreverc3againstlpsinducedinflammationinraw2647cellsandcarrageenaninducedpawedema AT shyamaprasadkodimule efficacyofastandardizedturmericextractcomprisedof70bisdemothoxycurcuminreverc3againstlpsinducedinflammationinraw2647cellsandcarrageenaninducedpawedema |