Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro

This in vitro study evaluated the protective effect of titanium tetrafluoride (TiF(4)) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF(4) varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control). Microcosm biofilm was produced from human dental biofilm (from patients with head-neck cancer) mixed with McBain saliva for the first 8 h. After 16 h and from day 2 to day 5, McBain saliva (0.2% sucrose) was replaced daily (37 °C, 5% CO(2)) (biological triplicate). Demineralization was quantified by transverse microradiography (TMR), while biofilm was analyzed by using viability, colony-forming units (CFU) counting and lactic acid production assays. The data were statistically analyzed by ANOVA (p < 0.05). TiF(4) and SDF were able to reduce mineral loss compared to placebo and the negative control. TiF(4) and SDF significantly reduced the biofilm viability compared to negative control. TiF(4) significantly reduced the CFU count of total microorganism, while only SDF affected total streptococci and mutans streptococci counts. The varnishes induced a reduction in lactic acid production compared to the negative control. TiF(4) and SDF may be good alternatives to control the development of radiation-induced dentin caries.