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Cell wall N-glycan of Candida albicans ameliorates early hyper- and late hypo-immunoreactivity in sepsis
Severe infection often causes a septic cytokine storm followed by immune exhaustion/paralysis. Not surprisingly, many pathogens are equipped with various anti-inflammatory mechanisms. Such mechanisms might be leveraged clinically to control septic cytokine storms. Here we show that N-glycan from pat...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966402/ https://www.ncbi.nlm.nih.gov/pubmed/33727664 http://dx.doi.org/10.1038/s42003-021-01870-3 |
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author | Kawakita, Masataka Oyama, Taiki Shirai, Ikuma Tanaka, Shuto Akaki, Kotaro Abe, Shinya Asahi, Takuma Cui, Guangwei Itoh, Fumie Sasaki, Masato Shibata, Nobuyuki Ikuta, Koichi Hatakeyama, Tomomitsu Takahara, Kazuhiko |
author_facet | Kawakita, Masataka Oyama, Taiki Shirai, Ikuma Tanaka, Shuto Akaki, Kotaro Abe, Shinya Asahi, Takuma Cui, Guangwei Itoh, Fumie Sasaki, Masato Shibata, Nobuyuki Ikuta, Koichi Hatakeyama, Tomomitsu Takahara, Kazuhiko |
author_sort | Kawakita, Masataka |
collection | PubMed |
description | Severe infection often causes a septic cytokine storm followed by immune exhaustion/paralysis. Not surprisingly, many pathogens are equipped with various anti-inflammatory mechanisms. Such mechanisms might be leveraged clinically to control septic cytokine storms. Here we show that N-glycan from pathogenic C. albicans ameliorates mouse sepsis through immunosuppressive cytokine IL-10. In a sepsis model using lipopolysaccharide (LPS), injection of the N-glycan upregulated serum IL-10, and suppressed pro-inflammatory IL-1β, TNF-α and IFN-γ. The N-glycan also improved the survival of mice challenged by LPS. Analyses of structurally defined N-glycans from several yeast strains revealed that the mannose core is key to the upregulation of IL-10. Knocking out the C-type lectin Dectin-2 abrogated the N-glycan-mediated IL-10 augmentation. Furthermore, C. albicans N-glycan ameliorated immune exhaustion/immune paralysis after acute inflammation. Our results suggest a strategy where the immunosuppressive mechanism of one pathogen can be applied to attenuate a severe inflammation/cytokine storm caused by another pathogen. |
format | Online Article Text |
id | pubmed-7966402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79664022021-04-01 Cell wall N-glycan of Candida albicans ameliorates early hyper- and late hypo-immunoreactivity in sepsis Kawakita, Masataka Oyama, Taiki Shirai, Ikuma Tanaka, Shuto Akaki, Kotaro Abe, Shinya Asahi, Takuma Cui, Guangwei Itoh, Fumie Sasaki, Masato Shibata, Nobuyuki Ikuta, Koichi Hatakeyama, Tomomitsu Takahara, Kazuhiko Commun Biol Article Severe infection often causes a septic cytokine storm followed by immune exhaustion/paralysis. Not surprisingly, many pathogens are equipped with various anti-inflammatory mechanisms. Such mechanisms might be leveraged clinically to control septic cytokine storms. Here we show that N-glycan from pathogenic C. albicans ameliorates mouse sepsis through immunosuppressive cytokine IL-10. In a sepsis model using lipopolysaccharide (LPS), injection of the N-glycan upregulated serum IL-10, and suppressed pro-inflammatory IL-1β, TNF-α and IFN-γ. The N-glycan also improved the survival of mice challenged by LPS. Analyses of structurally defined N-glycans from several yeast strains revealed that the mannose core is key to the upregulation of IL-10. Knocking out the C-type lectin Dectin-2 abrogated the N-glycan-mediated IL-10 augmentation. Furthermore, C. albicans N-glycan ameliorated immune exhaustion/immune paralysis after acute inflammation. Our results suggest a strategy where the immunosuppressive mechanism of one pathogen can be applied to attenuate a severe inflammation/cytokine storm caused by another pathogen. Nature Publishing Group UK 2021-03-16 /pmc/articles/PMC7966402/ /pubmed/33727664 http://dx.doi.org/10.1038/s42003-021-01870-3 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kawakita, Masataka Oyama, Taiki Shirai, Ikuma Tanaka, Shuto Akaki, Kotaro Abe, Shinya Asahi, Takuma Cui, Guangwei Itoh, Fumie Sasaki, Masato Shibata, Nobuyuki Ikuta, Koichi Hatakeyama, Tomomitsu Takahara, Kazuhiko Cell wall N-glycan of Candida albicans ameliorates early hyper- and late hypo-immunoreactivity in sepsis |
title | Cell wall N-glycan of Candida albicans ameliorates early hyper- and late hypo-immunoreactivity in sepsis |
title_full | Cell wall N-glycan of Candida albicans ameliorates early hyper- and late hypo-immunoreactivity in sepsis |
title_fullStr | Cell wall N-glycan of Candida albicans ameliorates early hyper- and late hypo-immunoreactivity in sepsis |
title_full_unstemmed | Cell wall N-glycan of Candida albicans ameliorates early hyper- and late hypo-immunoreactivity in sepsis |
title_short | Cell wall N-glycan of Candida albicans ameliorates early hyper- and late hypo-immunoreactivity in sepsis |
title_sort | cell wall n-glycan of candida albicans ameliorates early hyper- and late hypo-immunoreactivity in sepsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966402/ https://www.ncbi.nlm.nih.gov/pubmed/33727664 http://dx.doi.org/10.1038/s42003-021-01870-3 |
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